mCherry Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these ...read more
Genetic Strategies: Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein ...read more
Western Blot: mCherry Antibody [NBP2-25157] - Analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit mCherry pAb, dilution 1:1000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells ...read more
Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Staining of a mouse melanoma with over expression of mCherry in lung tissue. Image from verified customer review.
Western Blot: mCherry Antibody [NBP2-25157] - Detection of mCherry-fused protein: MDA-MB-231 cell lysates with and without expression of mCherry-fused protein. Image from verified customer review.
Live Imaging Microscopy: mCherry Antibody [NBP2-25157] - Analysis of a Drosophila wing imaginal disc. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, All-NA, BaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, LIM, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

mCherry Antibody Summary

Immunogen
Full length recombinant mCherry protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Live Imaging Microscopy
Application Notes
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~28 kDa. Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 28891816 respectively). Use in Live Imaging Microscopy was reported from a verified customer review. Knockdown validation (PMID: 32494070).
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.8
using
NBP2-25157 in the following applications:

Publications
Read Publications using
NBP2-25157 in the following applications:

  • 10 publications
  • IHC
    5 publications
  • 5 publications
  • KD
    1 publication
  • WB
    6 publications

Reactivity Notes

Human, mouse, and rat reactivity reported in multiple pieces of scientific literature. Bacteria reactivity reported in scientific literature (PMID: 29649975).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol (1:1)
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for mCherry Antibody

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP2-25157)(31)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Drosophila, Invertebrate, Non-species specific.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, KD, WB.


Filter By Application
ICC/IF
(10)
IHC
(5)
IHC-P
(5)
KD
(1)
WB
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(6)
Rat
(3)
Bacteria
(1)
Drosophila
(4)
Invertebrate
(1)
Non-species specific
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NBP2-25157 Applications Species
Miltner AM, Mercado-Ayon Y, Cheema SK et al. A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression Int J Mol Sci 2019 Jun 14 [PMID: 31197108] (IHC, Mouse) IHC Mouse
Agarwal E, Altman BJ, Seo JH, Bertolini I et al. MYC REGULATION OF A MITOCHONDRIAL TRAFFICKING NETWORK MEDIATES TUMOR CELL INVASION AND METASTASIS Mol. Cell. Biol. May 6 2019 [PMID: 31061095]
Xiang Y, Tanaka Y, Cakir B et al. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids Cell Stem Cell Feb 14 2019 [PMID: 30799279] (ICC/IF, Human) ICC/IF Human
Giovannone D, Paul S, Schindler S et al. Programmed conversion of hypertrophic chondrocytes into osteoblasts and marrow adipocytes within zebrafish bones Elife Feb 20 2019 [PMID: 30785394] (IHC-P) IHC-P
Ecker BL, kaur A, Douglass SM et al. Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis. Cancer Discov. Oct 2 2018 [PMID: 30279173] (IHC-P, Human, Mouse) IHC-P Human, Mouse
Maharjan DM, Dai YY, Glantz EH, Jadhav SP. Disruption of dorsal hippocampal - prefrontal interactions using chemogenetic inactivation impairs spatial learning. Neurobiol Learn Mem Sep 1 2018 [PMID: 30179661] (IHC, Rat) IHC Rat
Lui S, Torontali Z, Tadjalli A et al. Brainstem Nuclei Associated with Mediating Apnea-Induced Respiratory Motor Plasticity Sci Rep Aug 23 2018 [PMID: 30139983] (ICC/IF, Rat) ICC/IF Rat
Lui SKC Neural Circuitry of Upper Airway Respiratory Plasticity: Identifying the Neural Circuitry Underlying Long-term Facilitation of Inspiratory Genioglossus Motor Output Thesis
Zarin AA, Mark B, Cardona A A multilayer circuit architecture for the generation of distinct locomotor behaviors in Drosophila Elife Dec 23 2019 [PMID: 31868582] (ICC/IF, Drosophila) ICC/IF Drosophila
Gallo R, Rai A, Pelkmas L. DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway bioRxiv Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Show All 31 Publications.

Reviews for mCherry Antibody (NBP2-25157) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Drosophila.

Reviews using NBP2-25157:
Filter by Applications
WB
(2)
IF
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
Drosophila
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot mCherry NBP2-25157
Enlarge
reviewed by:
Feng Li
WB Human 11/18/2019
View

Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate, Sample Amount: 20ng
SpeciesHuman
Lot010519
Western Blot mCherry NBP2-25157
Enlarge
reviewed by:
Anonymous
WB Human 08/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman
Lot012016
 mCherry NBP2-25157
Enlarge
reviewed by:
Masahiko Takemura
Drosophila 12/08/2016
View

Summary

Sample TestedWing imaginal disc
SpeciesDrosophila
Lot22614

Comments

Comments1:1000 dilution
Immunofluorescence mCherry NBP2-25157
Enlarge
reviewed by:
Amanpreet Kaur
IF Mouse 07/23/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse melanoma with mCherry overexpression
SpeciesMouse
Lot22614

Blocking

Blocking DetailsHydrogen peroxide block for 20 minutes (Ultravision)

Primary Anitbody

Dilution Ratio1:500 at 4 degrees Celsius overnight. Antibody was diluted in Antibody Diluent (Dako)

Secondary Antibody

Secondary DescriptionGoat anti-rabbit (Ultravision)
Secondary Manufacturer Cat#TR-060-BN (Fisher)

Details

Detection NotesAEC Chromogen - #TA060SA
Fixation DetailsFixation -10% formalin, antigen retrival - citrate buffer followed by steaming for 20 minutes.
Wash Description1x PBS for 5 minutes 3 times.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 2 of 2 FAQs).

  1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
  2. I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato?
    • We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins.   The antibody is a polycloanl which then increases the likelihood that it will have multiple binding sites along the homologous sequences.    There really is no regions of sequence where one could make an antibody that did not see both proteins.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-25157AF350
Alexa Fluor 405 NBP2-25157AF405
Alexa Fluor 488 NBP2-25157AF488
Alexa Fluor 532 NBP2-25157AF532
Alexa Fluor 594 NBP2-25157AF594
Alexa Fluor 647 NBP2-25157AF647
Alexa Fluor 700 NBP2-25157AF700
Alexa Fluor 750 NBP2-25157AF750
Biotin NBP2-25157B
DyLight 350 NBP2-25157UV
DyLight 405 NBP2-25157V
DyLight 488 NBP2-25157G
DyLight 550 NBP2-25157R
DyLight 594 NBP2-25157DL594
DyLight 650 NBP2-25157C
DyLight 680 NBP2-25157FR
DyLight 755 NBP2-25157IR
FITC NBP2-25157F
HRP NBP2-25157H
Janelia Fluor 549 NBP2-25157JF549
Janelia Fluor 646 NBP2-25157JF646

Additional mCherry Products

Array NBP2-25157

Research Areas for mCherry Antibody (NBP2-25157)

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Contact Information

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Calculators

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Feng Li
11/18/2019
Application: WB
Species: Human

Anonymous
08/22/2017
Application: WB
Species: Human

Masahiko Takemura
12/08/2016
Application:
Species: Drosophila