mCherry Antibody

Images

 
Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with ...read more
Immunohistochemistry: mCherry Antibody [NBP2-25157] - Activation of TRPV1 receptors in ARC POMC neurons reduces food intake. Expression of Gi/o-DREADD in POMC neurons in the ARC (top left panel, scale bar: 20 um). ...read more
Western Blot: mCherry Antibody [NBP2-25157] - Analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit mCherry pAb, dilution 1:1000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells ...read more
Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in the adult retina. Flat-mounted retina labeled with anti-mCherry antibody. (BAE ) mCherry (red) and Brn3 (teal) colocalization. ...read more
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. (A) Optic tracts at P0 visualized by mCherry staining (white arrows). (B,BAE ) ...read more
Western Blot: mCherry Antibody [NBP2-25157] - PKN3 overexpression regulates growth of MEFs, and this effect requires PKN3AE p130Cas interaction. Immunoblotted lysates from MEFs p130CasA/A or MEFs p130CasA/A reAE ...read more
Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Staining of a mouse melanoma with over expression of mCherry in lung tissue. IHC-P image submitted by a verified customer review.
Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein band in lane marked '-'.
Western Blot: mCherry Antibody [NBP2-25157] - Detection of mCherry-fused protein: MDA-MB-231 cell lysates with and without expression of mCherry-fused protein. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these ...read more
Fluorescence Imaging: mCherry Antibody [NBP2-25157] - Analysis of a Drosophila wing imaginal disc. Image submitted by a verified customer review.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, LIM, FI, IHC-WhMt, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

mCherry Antibody Summary

Immunogen
This mCherry Antibody was developed against full length recombinant mCherry protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Fluorescence Imaging
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Live Imaging Microscopy
  • Western Blot 1:1000
Application Notes
Use in Immunohistochemistry Whole-Mount reported in scientific literature (PMID:35013168) This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~28 kDa.
Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 28891816 respectively).
Use in Live Imaging Microscopy was reported from a verified customer review.
Knockdown validation (PMID: 32494070).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-25157 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 50% glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for mCherry Antibody

  • DSRED
  • red fluorescent protein mCherry
  • Red Fluoroscent Protein

Background

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).

mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).

References

1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819

2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83

3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992

4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026

5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP2-25157)(53)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Drosophila, Invertebrate, Non-species specific.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-P, IHC-WhMt, KD, WB.


Filter By Application
ICC/IF
(12)
IHC
(11)
IHC-P
(5)
IHC-WhMt
(1)
KD
(1)
WB
(11)
All Applications
Filter By Species
Human
(6)
Mouse
(6)
Rat
(3)
Bacteria
(1)
Drosophila
(4)
Invertebrate
(1)
Non-species specific
(1)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NBP2-25157 Applications Species
Gallo R, Rai A, Pelkmas L. DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway bioRxiv Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Sato H, Singer RH Cellular variability of nonsense-mediated mRNA decay Nature communications Dec 10 2021 [PMID: 34893608] (WB) WB
Mark B, Lai SL, Zarin AA et al. A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS eLife May 11 2021 [PMID: 33973523]
Rivera O, Sharma M, Shahani N et al. Rhes, a Striatal Enriched Protein, Regulates Post-Translational Small-Ubiquitin-like-Modifier (SUMO) Modification of Nuclear Proteins and Alters Gene Expression bioRxiv Jun 19 2020 (WB) WB
Weeraratna A, Fane M, Douglass S et al. Stromal Changes In The Aged Lung Induce An Emergence From Melanoma Dormancy Research Square Sep 16 2020 (IHC) IHC
Troyanovsky RB, Indra I, Kato R Et al. Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity The Journal of biological chemistry Oct 8 2021 [PMID: 34634305] (ICC/IF) ICC/IF
He L, Huang Z, Huang K et al. Optogenetic Control of Non Apoptotic Cell Death Advanced Science May 6 2021

Details:
A lipid strip assay was performed using the mCherry antibody on human HeLa cells. This confirmed the light dependent interaction between LiPOP1 and various phospholipids on a nitrocellulose membrane.
Nakamura T, Kurosaki K, Kanemoto M et al. Early-life experiences altered the maturation of the lateral habenula in mouse models, resulting in behavioural disorders in adulthood J Psychiatry Neurosci Aug 4 2021 [PMID: 34346201] (IHC) IHC
Majolee J, Podieh F, Hordijk Pl, Kovacevic I The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading PloS one Jul 12 2021 [PMID: 34252134]
Yu H, Shi L, Chen J Et Al. A Neural Circuit Mechanism Controlling Breathing by Leptin in the Nucleus Tractus Solitarii Neuroscience bulletin Jul 2 2021 [PMID: 34212297]
Show All 53 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 4 of 4 FAQs).

  1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
  2. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
    • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
  3. I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato? 
    • We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins. The antibody is a polyclonal, which then increases the likelihood that it will have multiple binding sites along the homologous sequences. There really are no regions of sequence where one could make an antibody that did not see both proteins.
  4. I would like to know the isotype of our NBP2-25157 and the secondary antibody that would be suggested for it.
    • This antibody is a rabbit polyclonal of the isotype IgG. Please find here a link to our rabbit IgG isotype controls: http://www.novusbio.com/product-type/isotype-controls?fq[common_name]=Rabbit%2520IgGOf these products, NB810-56910 should meet your requirements.

Secondary Antibodies

 

Isotype Controls

Additional mCherry Products

Array NBP2-25157

Research Areas for mCherry Antibody (NBP2-25157)

Find related products by research area.

Blogs on mCherry.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Autophagy and RAS signaling: Clinical implications
By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ...  Read full blog post.

Autophagic flux: Is p62 a good indicator?
By Christina Towers, PhD Is p62 a good indicator of autophagic flux? The short answer: Yes … but … SQSTM1 encodes the cargo adaptor protein, p62, which interacts with autophagic substrates and delivers them to aut...  Read full blog post.

Make each cell count: How to assess autophagy using flow cytometry
Kristy R. Howell, PhDThe cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamyci...  Read full blog post.

How to visualize autophagy by microscopy
By Christina Towers, PhD Autophagy is a recycling process that relies on the formation of a unique organelle termed an autophagosome. An elegant way to monitor autophagy is through various microscopy techniques to...  Read full blog post.

Best way to quantitatively measure Autophagic Flux
By Christina Towers, PhD Autophagy is a stress-induced cellular recycling process that plays an important physiological role in many diseases. It is induced by a variety of stimuli, both intracellular and extracel...  Read full blog post.

Animal Models to Study Autophagy
By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the...  Read full blog post.

Application Focus: I see an increase in LC3, now what?
 By Christina Towers, PhD.  Autophagy is highly conserved and tightly regulated process that all cell types use to recycle nutrients, particularly in the instance of stress1. As a result, even sm...  Read full blog post.

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