mCherry Antibody

Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.

Immunohistochemistry: mCherry Antibody [NBP2-25157] - Activation of TRPV1 receptors in ARC POMC neurons reduces food intake. Expression of Gi/o-DREADD in POMC neurons in the ARC (top left panel, scale bar: 20 um). Arrowheads represent POMC neurons that co-expressed mCherry (n = 493 out of 529 mCherry-positive neurons, n = 3 mice). Treatment with CNO (10 uM) hyperpolarized POMC neurons (top right panel; Control, -40.7 +/- 1.5 mV; CNO, -47.1 +/- 2.3 mV, n = 6 neurons, **p < 0.01). Scale bar: 25 mV, 2 min. Bottom panel: In the presence of CNO, treatment of the TRPV1 agonist was not able to depolarize POMC neurons (n = 3 neurons). Scale bar: 20 mV, 100 s. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pbio.2004399) licensed under a CC-BY license.

Western Blot: mCherry Antibody [NBP2-25157] - Analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit mCherry pAb, dilution 1:1000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein (predicted molecular weight ~27 kDa), [5] GFP recombinant protein, and [6] HEK293 transfected with a full length GFP construct. A major band at about 28kDa corresponds to mCherry protein. The RPCA-mCherry antibody does not react with GFP protein. The same blot was simultaneously probed with chicken HSP60 pAb, dilution 1:5000 (Red) which reveals a band at 60kDa only in cell lysates.

Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in the adult retina. Flat-mounted retina labeled with anti-mCherry antibody. (BAE ) mCherry (red) and Brn3 (teal) colocalization. Yellow arrow indicates a Brn3+ mCherry-cell. (CAE ) mCherry (red) and RBPMS (teal) colocalization. Yellow arrows indicate RBPMS+ mCherry-cell bodies. (DAE ) mCherry (red) and Tuj1 (teal) colocalization. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. (A) Optic tracts at P0 visualized by mCherry staining (white arrows). (B,BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (C,CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Low magnification image of a horizontal E13. 5 whole-head section. EAE

Western Blot: mCherry Antibody [NBP2-25157] - PKN3 overexpression regulates growth of MEFs, and this effect requires PKN3AE p130Cas interaction. Immunoblotted lysates from MEFs p130CasA/A or MEFs p130CasA/A reAE expressing p130Cas (p130Cas+) treated by Doxycycline (Dox) to induce expression of mCherryAE PKN3 or mCherry alone. p130Cas presence was detected by aniAE p130Cas antibody and mCherry epitope by anti mCherry antibody. Dynamics of mCherry PKN3 expression after supplementation with Dox shown by immunoblot with anti mCherry antibody. Image collected and cropped by Citeab from the following publication (The interaction of p130Cas with PKN3 promotes malignant growth. Mol Oncol (2019)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Staining of a mouse melanoma with over expression of mCherry in lung tissue. IHC-P image submitted by a verified customer review.

Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein band in lane marked '-'.

Western Blot: mCherry Antibody [NBP2-25157] - Detection of mCherry-fused protein: MDA-MB-231 cell lysates with and without expression of mCherry-fused protein. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.

Fluorescence Imaging: mCherry Antibody [NBP2-25157] - Analysis of a Drosophila wing imaginal disc. Image submitted by a verified customer review.

• Reactivity: All-NA
• Applications: WB, ICC/IF, IHC, MS, IHC, KD, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mCherry Antibody Summary

• Immunogen: This mCherry Antibody was developed against full length recombinant mCherry protein
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Fluorescence Imaging
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Live Imaging Microscopy
  • Western Blot 1:1000
• Application Notes: Use in Immunohistochemistry Whole-Mount reported in scientific literature (PMID:35013168) This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~28 kDa.
  Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 28891816 respectively).
  Use in Live Imaging Microscopy was reported from a verified customer review.
  Knockdown validation (PMID: 32494070).
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mCherry Antibody

• DSRED
• mCherry
• red fluorescent protein mCherry
• Red Fluoroscent Protein

Background

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy (1). mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone) (2). The red chromophore of DsRed has a similar topology to GFP, the green fluorescent protein isolated from the jellyfish Aequorea Victoria, but has extended pi-electron conjugation resulting in red-shifted absorbance and emission (3). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).

mCherry can be used as a long-wavelength hetero-FRET (fluorescence resonance energy transfer) acceptor and probe for homoFRET experiments given its high peak molar absorptivity, folding efficiency, and superior spectral properties (4). Additionally, because mCherry does not interfere with other plasmids or alter the growth of Legionella species during intracellular growth, it can be used for constitutive gene expression in a variety of gram-negative bacterial species (5). For example, a plasmid developed to constitutively express mCherry under the Ptac promoter has been used in several Legionella species including L. pneumophila, the causative agent of Legionnaires' disease (5).

References

1. Shaner, N. C., Steinbach, P. A., & Tsien, R. Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2(12), 905-909. doi:10.1038/nmeth819

2. Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature Biotechnology, 20(1), 83-87. https://doi.org/10.1038/nbt0102-83

3. Wall, M. A., Socolich, M., & Ranganathan, R. (2000). The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nature Structural Biology, 7(12), 1133-1138. https://doi.org/10.1038/81992

4. Akrap, N., Seidel, T., & Barisas, B. G. (2010). Forster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins. Analytical Biochemistry, 402(1), 105-106. https://doi.org/10.1016/j.ab.2010.03.026

5. Gebhardt, M. J., Jacobson, R. K., & Shuman, H. A. (2017). Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria. Plos One, 12(3), e0173116. https://doi.org/10.1371/journal.pone.0173116

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mCherry Antibody (NBP2-25157)(60)

Publications using NBP2-25157

• Rivera O, Sharma M, Shahani N et al. Rhes, a Striatal Enriched Protein, Regulates Post-Translational Small-Ubiquitin-like-Modifier (SUMO) Modification of Nuclear Proteins and Alters Gene Expression bioRxiv 2020-06-19 (WB)
• Lui SKC Neural Circuitry of Upper Airway Respiratory Plasticity: Identifying the Neural Circuitry Underlying Long-term Facilitation of Inspiratory Genioglossus Motor Output Thesis
• Soubeyrand S, Lau P, McPherson R Regulation of TRIB1 abundance in hepatocyte models in response to proteasome inhibition Scientific reports 2023-06-08 [PMID: 37291259] (IP)
• Fane ME, Chhabra Y, Alicea GM et al. Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature 2022-06-01 [PMID: 35650435]
• Lee KM, Linskens AM, Doe CQ Hunchback activates Bicoid in Pair1 neurons to regulate synapse number and locomotor circuit function Current biology : CB 2022-04-29 [PMID: 35512697]
• Srivastava PN, Mishra S Disrupting a Plasmodium berghei putative phospholipase impairs efficient egress of merosomes International journal for parasitology 2022-04-12 [PMID: 35427579]
• Komachi K, Burgess SM The Nup2 meiotic-autonomous region relieves inhibition of Nup60 to promote progression of meiosis and sporulation in Saccharomyces cerevisiae Genetics 2022-03-18 [PMID: 35302609] (ICC/IF)
• Lawrence M, Elhendawi M, Morlock M et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity iScience 2022-03-01 [PMID: 35243244]
• Fabian P, Tseng KC, Thiruppathy M et al. Lifelong single-cell profiling of cranial neural crest diversification in zebrafish Nature communications 2022-01-10 [PMID: 35013168] (IHC-WhMt)
• Sato H, Singer RH Cellular variability of nonsense-mediated mRNA decay Nature communications 2021-12-10 [PMID: 34893608] (WB)
• Troyanovsky RB, Indra I, Kato R Et al. Basolateral protein Scribble binds phosphatase PP1 to establish a signaling network maintaining apicobasal polarity The Journal of biological chemistry 2021-10-08 [PMID: 34634305] (ICC/IF)
• Nakamura T, Kurosaki K, Kanemoto M et al. Early-life experiences altered the maturation of the lateral habenula in mouse models, resulting in behavioural disorders in adulthood J Psychiatry Neurosci 2021-08-04 [PMID: 34346201] (IF/IHC)
• Majolee J, Podieh F, Hordijk Pl, Kovacevic I The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreading PloS one 2021-07-12 [PMID: 34252134]
• Yu H, Shi L, Chen J Et Al. A Neural Circuit Mechanism Controlling Breathing by Leptin in the Nucleus Tractus Solitarii Neuroscience bulletin 2021-07-02 [PMID: 34212297]
• Holdhof D, Schoof M, Al-Kershi S et al. Brahma-related gene 1 has time-specific roles during brain and eye development Development (Cambridge, England) 2021-05-15 [PMID: 34042968]
• Fane, M E, Ecker, B L Et al. sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients. Clin Cancer Res 2020-11-01 [PMID: 33097493] (WB)
• Burbidge, K, Zwikelmaier, V Et al. Cargo and cell-specific differences in extracellular vesicle populations identified by multiplexed immunofluorescent analysis. J Extracell Vesicles 2020-07-17 [PMID: 32944176] (WB)
• Rosenthal, ZP; Influence of Focal Activity on Macroscale Brain Dynamics in Health and Disease Thesis (IF/IHC)
• He L, Huang Z, Huang K et al. Optogenetic Control of Non Apoptotic Cell Death Adv Sci (Weinh) 2021-09-20 [PMID: 34540558]
• Mark B, Lai SL, Zarin AA et al. A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS eLife 2021-05-11 [PMID: 33973523]
• He L, Tan P, Zhu L et al. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering Nature chemical biology 2021-05-06 [PMID: 33958793]
• Ackerman SD, Perez-Catalan NA, Freeman MR, Doe CQ Astrocytes close a motor circuit critical period Nature 2021-04-01 [PMID: 33828296] (IF/IHC)
• Fielden LF, Scott NE, Palmer CS, et al. Proteomic identification of Coxiella burnetii effector proteins targeted to the host cell mitochondria during infection Molecular & cellular proteomics : MCP 2020-11-11 [PMID: 33177156]
• Valdes-Aleman J, Fetter RD, Sales EC et al. Comparative Connectomics Reveals How Partner Identity, Location, and Activity Specify Synaptic Connectivity in Drosophila Neuron 2020-10-20 [PMID: 33120017] (IF/IHC)
• Werner CT, Mitra S, Auerbach BD et al. Neuroadaptations in the dorsal hippocampus underlie cocaine seeking during prolonged abstinence Proc Natl Acad Sci U S A 2020-10-10 [PMID: 33020308] (ICC/IF)
• von Appen A, LaJoie D, Johnson I et al. LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation Nature 2020-04-29 [PMID: 32494070] (KD, WB)
• Zarin AA, Mark B, Cardona A A multilayer circuit architecture for the generation of distinct locomotor behaviors in Drosophila Elife 2019-12-23 [PMID: 31868582] (ICC/IF, Drosophila)
• Batabyal S, Gajjeraman S Nano-enhanced optical gene delivery to retinal degenerated mice. Curr Gene Ther 2019-10-17 [PMID: 31625475] (IF/IHC)
• Batenburg NL, Walker JR, Coulombe Y et al. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection Nucleic Acids Res. [PMID: 31501894] (WB)
• Gallo R, Rai A, Pelkmas L. DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway Cells 2019-09-01 [PMID: 31470564]
• Miltner AM, Mercado-Ayon Y, Cheema SK et al. A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression Int J Mol Sci 2019-06-14 [PMID: 31197108] (IF/IHC, Mouse)
• Xiang Y, Tanaka Y, Cakir B et al. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids Cell Stem Cell 2019-02-14 [PMID: 30799279] (ICC/IF, Human)
• Lui S, Tadjali A, Peever J Multiple Hypoxia-Independent Triggers of Upper Airway Long-Term Facilitation Research Square 2023-05-03 (IHC)
• Rosenthal Zp, Raut Rv, Yan P Et Al. Local Perturbations of Cortical Excitability Propagate Differentially Through Large-Scale Functional Networks Cereb. Cortex 2020-02-10 [PMID: 32043145] (IF/IHC)
• Agarwal E, Altman BJ, Seo JH, Bertolini I et al. MYC REGULATION OF A MITOCHONDRIAL TRAFFICKING NETWORK MEDIATES TUMOR CELL INVASION AND METASTASIS Mol. Cell. Biol. 2019-05-06 [PMID: 31061095]
• Sales, EC;Heckman, EL;Warren, TL;Doe, CQ; Regulation of subcellular dendritic synapse specificity by axon guidance cues Elife 2019-04-23 [PMID: 31012844] (IF/IHC, Drosophila)
• Giovannone D, Paul S, Schindler S et al. Programmed conversion of hypertrophic chondrocytes into osteoblasts and marrow adipocytes within zebrafish bones Elife 2019-02-20 [PMID: 30785394] (IHC-P)
• Gemperle J, Dibus M, Koudelkova L et al. The interaction of p130Cas with PKN3 promotes malignant growth Mol Oncol. [PMID: 30422386] (WB, Mouse)
• Ecker BL, kaur A, Douglass SM et al. Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis. Cancer Discov. 2018-10-02 [PMID: 30279173] (IHC-P, Mouse, Human)
• Maharjan DM, Dai YY, Glantz EH, Jadhav SP. Disruption of dorsal hippocampal - prefrontal interactions using chemogenetic inactivation impairs spatial learning. Neurobiol Learn Mem 2018-09-01 [PMID: 30179661] (IF/IHC, Rat)
• Uhia I, Krishnan N, Robertson BD. Characterising resuscitation promoting factor fluorescent-fusions in mycobacteria BMC Microbiol. 2018-04-12 [PMID: 29649975] (WB, Bacteria)
• Hinaux H, Bachem K, Battistara M et al. Revisiting the developmental and cellular role of the pigmentation gene yellow in Drosophila using a tagged allele. Dev. Biol. 2018-04-07 [PMID: 29634916] (Drosophila)
• Burbidge K, Zwikelmaier V, Cook B, Ispas G Cargo and Cell Specific Differences in Extracellular Vesicle Populations Identified by Multiplexed Immunofluorescent Analysis BioRxiv (WB, Human)
• Lui S, Torontali Z, Tadjalli A et al. Brainstem Nuclei Associated with Mediating Apnea-Induced Respiratory Motor Plasticity Sci Rep 2018-08-23 [PMID: 30139983] (ICC/IF, Rat)
• Carreira-Rosario A, Zarin AA, Clark MQ et al. MDN brain descending neurons coordinately activate backward and inhibit forward locomotion Elife 2018-08-02 [PMID: 30070205] (ICC/IF, Drosophila)
• Seo JH, Agarwal E, Bryant KG et al. Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics And Tumor Cell Movements Cancer Res. 2018-06-13 [PMID: 29898993] (IHC-P, Mouse)
• Jeong JH, Lee DK, Liu SM et al. Activation of temperature-sensitive TRPV1-like receptors in ARC POMC neurons reduces food intake PLoS Biol. 2018-04-01 [PMID: 29689050] (ICC/IF, Mouse)
• Singh H R, Nardozza A P et al. A Poly-ADP-Ribose Trigger Releases the Auto-Inhibition of a Chromatin Remodeling Oncogene. Mol Cell 2017-07-12 [PMID: 29220653] (WB)
• Xu X, Iwasa H, Hossain S et al. BCL-XL binds and antagonizes RASSF6 tumor suppressor to suppress p53 expression Genes Cells. 2017-11-28 [PMID: 29193479] (Invertebrate)
• Tripathy D, Vignoli B, Ramesh N et al. Mutations in TGM6 induce the unfolded protein response in SCA35 Hum Mol Genet. 2017-10-01 [PMID: 28934387] (WB)
• Caino MC, Seo JH, Wang Y et al. Syntaphilin controls a mitochondrial rheostat for proliferation-motility decisions in cancer J. Clin. Invest. 2017-09-11 [PMID: 28891816] (IHC-P, Mouse)
• Lee I, Kim GS, Bae JS et al. The DNA replication protein Cdc6 inhibits the microtubule-organizing activity of the centrosome J. Biol. Chem. 2017-08-21 [PMID: 28827311] (ICC/IF, Human)
• Takemura M, Nakato H. Drosophila Sulf1 is required for the termination of intestinal stem cell division during regeneration. J. Cell. Sci. 2016-11-25 [PMID: 27888216] (IF/IHC)
• Li A The Influence of Ontogeny and Physiological Microbial Exposure on Macrophage Maintenance and Function Thesis (ICC/IF, Non-species specific)
• Cho A, Kato M, Whitwam T et al. An Atypical Tropomyosin in Drosophila with Intermediate Filament-like Properties Cell Rep 2016-07-26 [PMID: 27396338] (IF/IHC)
• Kesireddy V. Evaluation of adipose-derived stem cells for tissue-engineered muscle repair construct-mediated repair of a murine model of volumetric muscle loss injury. Int J Nanomedicine. 2016-04-26 [PMID: 27114706] (IHC-P, Rat)
• He L, Zhang Y, Ma G et al. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife. 2015-12-29 [PMID: 26646180] (ICC/IF, Human)
• Jing J, He L, Sun A et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca2+ influx. NATURE CELL BIOLOGY 2015-08-31 [PMID: 26322679]
• Jackson BC, Ivanova IA, Dagnino L. AN ELMO2-RHOG-ILK NETWORK MODULATES MICROTUBULE DYNAMICS. Mol. Biol. Cell. 2015-05-20 [PMID: 25995380] (ICC/IF)

Reviews for mCherry Antibody (NBP2-25157) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Mouse, Drosophila.

reviewed by: Verified Customer WB Human 11/18/2019

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate, Sample Amount: 20ng
• Species: Human
• Lot: 010519

reviewed by: Michal Dibus WB Human 08/22/2017

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231 Cell Lysate
• Species: Human
• Lot: 012016

reviewed by: Verified Customer Immunohistochemistry (whole mount) Drosophila 12/08/2016

Summary

• Application: Other
• Sample Tested: Wing imaginal disc
• Species: Drosophila
• Lot: 22614

Comments

• Comments: 1:1000 dilution

reviewed by: Verified Customer IF Mouse 07/23/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse melanoma with mCherry overexpression
• Species: Mouse
• Lot: 22614

FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 4 of 4 FAQs).

1. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
   • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
2. Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
   • mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
3. I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato? 
   • We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins. The antibody is a polyclonal, which then increases the likelihood that it will have multiple binding sites along the homologous sequences. There really are no regions of sequence where one could make an antibody that did not see both proteins.
4. I would like to know the isotype of our NBP2-25157 and the secondary antibody that would be suggested for it.
   • This antibody is a rabbit polyclonal of the isotype IgG. Of our rabbit IgG isotype control products, NB810-56910 should meet your requirements.