Immunocytochemistry/ Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these ...read more
Genetic Strategies: Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein ...read more
Western Blot: mCherry Antibody [NBP2-25157] - Analysis of HEK293 cell lysates, and recombinant protein solutions using rabbit mCherry pAb, dilution 1:1000 (Green).  protein standard,  HEK293,  HEK293 cells ...read more
Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Staining of a mouse melanoma with over expression of mCherry in lung tissue. Image from verified customer review.
Western Blot: mCherry Antibody [NBP2-25157] - Detection of mCherry-fused protein: MDA-MB-231 cell lysates with and without expression of mCherry-fused protein. Image from verified customer review.
Live Imaging Microscopy: mCherry Antibody [NBP2-25157] - Analysis of a Drosophila wing imaginal disc. Image from verified customer review.
This mCherry antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~28 kDa. Use in IHC and IHC-P reported in scientific literature (PMID: 27396338 and 28891816 respectively). Use in Live Imaging Microscopy was reported from a verified customer review. Knockdown validation (PMID: 32494070).
28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 4 Reviews rated 4.8 using NBP2-25157 in the following applications:
Human, mouse, and rat reactivity reported in multiple pieces of scientific literature. Bacteria reactivity reported in scientific literature (PMID: 29649975).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 50% glycerol (1:1)
5mM Sodium Azide
Immunogen affinity purified
Alternate Names for mCherry Antibody
red fluorescent protein mCherry
Red Fluoroscent Protein
mCherry represents an engineered derivative of a family of proteins initially isolated from a group of sea organisms called Cnidarians (jelly fish, sea anemones and corals). The prototype for these fluorescent proteins is GFP which was originally isolated from jellyfish Aequoria Victoria and when expressed in-vitro or in-vivo using its gene, GFP protein fold correctly with retention of its capability to deliver strong fluorescence. Engineered forms of GFP and its relatives have been developed to monitor several biological proresses such as Ca2+ levels, protease activation and a variety of other processes in real time. mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but, produces a red rather than a green fluoresnecce and its engineered monomeric form has several applications including FRET (Forster Resonance Energy Transfer). mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and it is also a monomeric form with an excitation maximum at 587 nm and an emission maximum at 610 nm.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for mCherry Antibody (NBP2-25157)(31)
We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bacteria, Drosophila, Invertebrate, Non-species specific.
We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, KD, WB.
Rivera O, Sharma M, Shahani N et al. Rhes, a Striatal Enriched Protein, Regulates Post-Translational Small-Ubiquitin-like-Modifier (SUMO) Modification of Nuclear Proteins and Alters Gene Expression bioRxiv Jun 19 2020 (WB)
FAQs for mCherry Antibody (NBP2-25157). (Showing 1 - 2 of 2 FAQs).
Does this antibody cross-react with GFP epitopes? As I would like to use both GFP and mCherry antibodies during histochemistry I would not like them to cross-react.
mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.
I was wondering if you had any literature on whether the polyclonal mCherry antibody NBP2-25157 shows cross-reaction with td-tomato?
We do see 88% homology between the mCherry and the dTomato sequences and therefore, the antibody will likely see both of these RFP proteins. The antibody is a polycloanl which then increases the likelihood that it will have multiple binding sites along the homologous sequences. There really is no regions of sequence where one could make an antibody that did not see both proteins.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.