Alkaline Phosphatase, Bacterial Antibody [Biotin]

Images

 
Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at ...read more

Product Details

Summary
Reactivity EcSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Order Details

Alkaline Phosphatase, Bacterial Antibody [Biotin] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Alkaline Phosphatase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross-reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:4000-1:20000
Readout System
Reviewed Applications
Read 1 Review rated 4
using
NB600-500 in the following applications:

Publications
Read Publications using NB600-500.

Reactivity Notes

No reactivity occurs against mammalian forms of Alkaline Phosphatase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Alkaline Phosphatase, Bacterial Antibody [Biotin]

  • BAP
  • ECK0378
  • JW0374
  • Pho A
  • PhoA

Background

Bacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase, Bacterial Antibody (NB600-500)(2)

Review for Alkaline Phosphatase, Bacterial Antibody (NB600-500) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB600-500:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 11/01/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman
CommentsGood

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:10000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: Sigma, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: AP

Comments

CommentsGood

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FAQs for Alkaline Phosphatase, Bacterial Antibody (NB600-500) (0)

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Anonymous
11/01/2011
Application: WB
Species: Human