| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80819 | |||
| BSA Free | NB100-507SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68281SS | |||
| Azide and BSA Free | NBP2-80993 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74572SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50599-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62566-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0005.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW128) at 5 ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.")
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Detection of p300 in a HeLa nuclear extract using NB100-507 (1:250). ECL: 15 sec exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0003.jpg)
![Flow (Intracellular): KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW128) antibody NB100-507 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Intracellular-NB100-507-img0004.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Subcellular fractionation and localization of Src and p300 in Panc-1 cells. Cell lysates of equal total protein or Panc-1 subcellular fractions were subjected to immunoblotting analysis for Src, pSrc, p300, tubulin (cytoskeletal proteins), GAPDH (soluble cytoplasmic proteins), calnexin (endoplasmic reticulum), grim19 (mitochondria), histone H3 (chromatin), and lamin A/C (nuclear lamina). Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW128) [NB100-507] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW128) NB100-507 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Immunocytochemistry-Immunofluorescence-NB100-507-img0009.jpg)
![Flow Cytometry: KAT3B/p300 Antibody (RW128) [NB100-507] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW128) NB100-507 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Flow-Cytometry-NB100-507-img0010.jpg)
![Western Blot: KAT3B/p300 Antibody (RW128) [NB100-507] - Kinase active Src is present in the nuclei of Panc-1 cells in association with p300. Src and p300 co-association in Panc-1 cells. Immunoblotting analysis of Src or p300 immunecomplexes from Panc-1 nuclear isolates probing for Src or p300, or IgG control. Image collected and cropped by Citeab from the following publication (A novel nuclear Src and p300 signaling axis controls migratory and invasive behavior in pancreatic cancer. Oncotarget (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW128-Western-Blot-NB100-507-img0006.jpg)
![Immunohistochemistry-Paraffin: pan Cadherin Antibody [NB200-592] - Mouse cervical cancer tissue stained with N-Cadherin antibody (green). Nuclei were counterstained with DAPI (Blue). Antigen retrieval was done with pepsin treatment. Image from verified customer review.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-Immunohistochemistry-Paraffin-NB200-592-img0003.jpg "Immunohistochemistry-Paraffin: pan Cadherin Antibody [NB200-592] - Mouse cervical cancer tissue stained with N-Cadherin antibody (green). Nuclei were counterstained with DAPI (Blue). Antigen retrieval was done with pepsin treatment. Image from verified customer review.")
![Western Blot: pan Cadherin Antibody [NB200-592] - Whole extract of Chicken Cardiac Muscle were separated on SDS-PAGE and probed with Anti-Pan Cadherin antibody produced in Rabbit. The antibody was developed using 1:1000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:200 antibody2. 1:400 antibody3. Negative Control](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-Western-Blot-NB200-592-img0005.jpg)
![Immunohistochemistry: pan Cadherin Antibody [NB200-592] - Immunoperoxidase staining of a formalin-fixed, paraffin-embedded section of human heart using Rabbit Anti-Pan Cadherin.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-Immunohistochemistry-NB200-592-img0004.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0012.jpg "Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) mimic in rat vascular smooth muscle cells (A7r5) suppressed angiogenesis and proliferation but promoted senescence. Representative western blots depicting TERF1, TERF2, and quaking expression in mimic NC or miR-214 mimic transfected cells. Image collected and cropped by CiteAb from the following publication (//molmed.biomedcentral.com/articles/10.1186/s10020-020-00167-1), licensed under a CC-BY license.")
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0011.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0003.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - Detection of TRF1. 50 ug of total lysate each lane.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Western-Blot-NB110-68281-img0005.jpg)
![Flow Cytometry: TRF-1 Antibody (57-6) [NB110-68281] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Flow-Cytometry-NB110-68281-img0009.jpg)
![Simple Western: TRF-1 Antibody (57-6) [NB110-68281] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/TRF-1-Antibody-57-6-Simple-Western-NB110-68281-img0010.jpg)
![Western Blot: TRF-1 Antibody (57-6) [NB110-68281] - MicroRNA-214-3p (miR-214) antagomiR in rat vascular smooth muscle cells (A7r5) induced angiogenesis & proliferation but suppressed senescence. a Schematic of the protocol for transfection of A7r5 VSMCs with a miRNA antagomiR control (antagomiR NC) or a miR-214 antagomiR & processed at the indicated times. b The effects of miR-214 antagomiR on VSMC cell proliferation were tested by CCK-8 assay. c Angiogenetic mRNA expression levels of NOS3, VEGFA, CXCL12 & CXCR4 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). d Senescence-associated mRNA expression of TERT, TERF1 & TERF2 compared in cells transfected with antagomiR NC or miR-214 antagomiRs (n = 3). e, G Representative western blots depicting TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking expression in antagomiR NC or miR-214 antagomiRs transfected cells. f, h Normalized expression of TERF1, TERF2, p16INK4, p21CIP1, pRB, & quaking (n = 3). i Senescence-associated beta -galactosidase staining demonstrating senescence in SCR or miR-214 transfected cells. j Bar graphs show quantification of relative of SA-beta -gal positive cells (n = 3). Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01; ns, non-significance (Two-tailed Student’s t-test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32410577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-68281_mouse-monoclonal-trf-1-antibody-57-6-310202416212346.jpg)
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Western-Blot-H00121441-M05-img0002.jpg "Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).")
![Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Immunohistochemistry-Paraffin-H00121441-M05-img0005.jpg)
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-Western-Blot-H00121441-M05-img0001.jpg)
![ELISA: NEDD1 Antibody (7D10) [H00121441-M05] - Detection limit for recombinant GST tagged NEDD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEDD1-Antibody-7D10-ELISA-H00121441-M05-img0007.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0012.jpg "Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/abstract/7142), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NB500-161-img0008.jpg)
![Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAT3B/p300 Antibody [NB500-161] - Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Western-Blot-NB500-161-img0010.jpg)
![KAT3B/p300 Antibody [NB500-161] - Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody NB500-161 used for IP at 6 ug/mg lysate. p300 was also immunoprecipitated by a previous lot (lot NB500-161-1) of this antibody. For blotting immunoprecipitated p300, NB500-161 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-NB500-161-img0011.jpg)
![Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Western-Blot-NB110-74572-img0005.jpg "Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.")
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SG2NA (S68) [NB110-74572] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.](http://images.novusbio.com/fullsize/SG2NA-Antibody-S68-Immunocytochemistry-Immunofluorescence-NB110-74572-img0002.jpg)
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0002.jpg "Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.")
![Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/EGFR-Antibody-DH8-3-EGFRvIII-Flow-Cytometry-NBP2-50599-img0001.jpg)
![Flow Cytometry: Mouse Monoclonal EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - Analysis of glioblastoma cell lines using EGFR Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-50599_mouse-monoclonal-egfr-antibody-dh8-3-egfrviii-mutant-flow-cytometry-145202414346..png)
![Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/IGF-I-Antibody-Immunohistochemistry-Paraffin-NBP2-16929-img0003.jpg "Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp2-16929_rabbit-polyclonal-igf-i-igf-1-antibody-western-blot-26720247103..jpg)
![Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16929_rabbit-polyclonal-igf-i-igf-1-antibody-western-blot-267202471123..jpg)
![Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16929_rabbit-polyclonal-igf-i-igf-1-antibody-western-blot-267202471250..jpg)
![Immunocytochemistry/ Immunofluorescence: IGF-I/IGF-1 Antibody [NBP2-16929] - IGF-I/IGF-1 antibody detects IGF-I/IGF-1 protein at Golgi apparatus membrane by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IGF-I/IGF-1 protein stained by IGF-I/IGF-1 antibody (NBP2-16929) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-16929_rabbit-polyclonal-igf-i-igf-1-antibody-9102024205683.jpg)
![Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Western-Blot-NBP2-62566-img0002.jpg "Western Blot: APP Antibody (6E10) - Chimeric [NBP2-62566] - Anti-Amyloid beta antibody 6E10. Human brain cerebral cortex (A), frontal cortex (B) and cerebellum (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 6E10 (NBP2-62566) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Amyloid beta is ~35kDa, though this protein is known to form SDS-resistant low-molecular weight oligomers as seen in this blot (i.e. dimers, c.f. Kumar et al. PMID: 21527912). NBP2-62566 successfully detected aggregate human Amyloid beta in human brain cerebral cortex, frontal cortex and cerebellum lysate samples.")
![Immunohistochemistry-Paraffin: APP Antibody (6E10) - Chimeric [NBP2-62566] - Staining of human Alzheimer's disease hippocampus tissue using anti-Amyloid Beta antibody. 6E10 Anti-Amyloid Beta staining of paraffin embedded human hippocampus affected by Alzheimer's disease using the rabbit-chimeric version of 6E10 (NBP2-62566). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62566 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of amyloid plaques in the parahippocampal gyrus may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/APP-Antibody-6E10-Chimeric-Immunohistochemistry-Paraffin-NBP2-62566-img0001.jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - 6E10 staining reveals amyloid plaque in frontal cortex of AD mouse model. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-284202314243..jpg)
![Immunohistochemistry-Frozen: APP Antibody (6E10) - Chimeric [NBP2-62566] - Plaque staining in AD Mouse model (Hippocampus) - without formic acid pretreatment. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-62566_rabbit-monoclonal-app-antibody-6e10-chimeric-immunohistochemistry-frozen-672023103423..jpg)
![Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunocytochemistry-Immunofluorescence-NB100-783-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)")
![Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/ZCCHC11-TUT4-Antibody-Immunohistochemistry-Paraffin-NB100-783-img0002.jpg)
![Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Western-Blot-NBP2-24695-img0004.jpg "Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Cyclin-D1-Antibody-Immunohistochemistry-Paraffin-NBP2-24695-img0005.jpg)