| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-41503SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33176 | |||
| IHC-Prediluted | NBP2-44945 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77457SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27328SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48028 | |||
| Azide and BSA Free | NBP2-34637-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48055 | |||
| Azide and BSA Free | NBP3-11516 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19344SS | |||
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NB110-41503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Podocalyxin Like Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41503-img0007.jpg "Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NB110-41503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Podocalyxin Like Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Western-Blot-NBP2-29409-img0008.jpg "Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (DA7).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Confocal immunofluorescence image of MCF cells using Cytokeratin 18 Antibody (DA7). Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Immunocytochemistry-Immunofluorescence-NBP2-29409-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Formalin-paraffin human colon cancer stained with Cytokeratin 18 Ab (DA7). Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Immunohistochemistry-Paraffin-NBP2-29409-img0003.jpg)
![Western Blot: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Analysis of Cytokeratin 18 in HeLa lysate using Cytokeratin 18 (CK18) antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Western-Blot-NBP2-29409-img0002.jpg)
![Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Simple-Western-NBP2-29409-img0004.jpg)
![Simple Western: Cytokeratin 18 Antibody (DA7) [NBP2-29409] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DA7-Simple-Western-NBP2-29409-img0005.jpg)
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen I Antibody [NBP1-77457] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Collagen-I-Antibody-Immunohistochemistry-NBP1-77457-img0002.jpg "Immunohistochemistry: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen I Antibody [NBP1-77457] - Analysis of Collagen 1 in mouse epidermis using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: Collagen I alpha 1 Antibody [NBP1-77457] - Collagen 1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77457-img0004.jpg)
![Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.](http://images.novusbio.com/fullsize/NOD2-Antibody-Western-Blot-NBP2-27328-img0001.jpg "Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.")
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-27328-img0002.jpg)
![Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Western-Blot-NB100-2911-img0003.jpg "Western Blot: Ceramide Kinase Antibody [NB100-2911] - Detection of Ceramide Kinase in A549 cell lysate using NB100-2911.")
![Immunocytochemistry/Immunofluorescence: Ceramide Kinase Antibody [NB100-2911] - Ceramide Kinase antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Ceramide-Kinase-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2911-img0004.jpg)
![Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Western-Blot-NBP1-79022-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Flotillin-1-Antibody-Simple-Western-NBP1-79022-img0003.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Western-Blot-NB100-68210-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Detection of Human HCF1 on HeLa whole cell lysate using 100-68210. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68209.")
![<b>Independent Antibodies Validation. </b>Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpg)
![Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-68210_rabbit-polyclonal-host-cell-factor-1-hcfc1-antibody-310202415541972.jpg)
![Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Western-Blot-NBP2-44738-img0005.jpg "Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (HM47/A9) [NBP2-44738] - Immunofluorescence Analysis of Raji cells labeling CD79a with CD79A Antibody (HM47/A9). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-44738-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0004.jpg)
![Flow Cytometry: CD79A Antibody (HM47/A9) [NBP2-44738] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Flow-Cytometry-NBP2-44738-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human tonsil stained with CD79a antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0001.jpg)
![Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Western-Blot-NBP2-44947-img0008.jpg "Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DE-K18).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (DE-K18) Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Immunocytochemistry-Immunofluorescence-NBP2-44947-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Immunohistochemistry-Paraffin-NBP2-44947-img0001.jpg)
![Flow Cytometry: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Flow Cytometric Analysis of trypsinised MeOH-fixed HeLa cells using Cytokeratin 18 Antibody (DE-K18) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Flow-Cytometry-NBP2-44947-img0005.jpg)
![Western Blot: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Western Blot Analysis of MCF-7cell lysate using Cytokeratin 18 Antibody (DE-K18).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Western-Blot-NBP2-44947-img0007.jpg)
![Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Simple Western lane view shows a specific band for Cytokeratin 18 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Simple-Western-NBP2-44947-img0002.jpg)
![Simple Western: Cytokeratin 18 Antibody (DE-K18) [NBP2-44947] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 18 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-DE-K18-Simple-Western-NBP2-44947-img0003.jpg)
![Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP1-19344-img0005.jpg "Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.")
![Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-NBP1-19344-img0004.jpg)
![Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Simple-Western-NBP1-19344-img0006.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33604-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Gelsolin/GSN Antibody [NBP1-33604] - Paraformaldehyde-fixed HeLa, using Gelsolin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33604-img0005.jpg)
![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse urinary bladder dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunohistochemistry-Paraffin-NBP1-33604-img0019.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33604-img0007.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33604-img0011.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33604-img0013.jpg)
![Western Blot: Gelsolin/GSN Antibody [NBP1-33604] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Western-Blot-NBP1-33604-img0015.jpg)
![Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody [NBP1-33604] - Mouse brain. Gelsolin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Gelsolin-GSN-Antibody-Immunohistochemistry-Paraffin-NBP1-33604-img0009.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15971] - Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0003.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Immunohistochemistry-Paraffin-NBP2-15971-img0004.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cortactin-Antibody-Western-Blot-NBP2-15971-img0002.jpg)
![Immunohistochemistry-Paraffin: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-910202420131266.jpg)
![Immunocytochemistry/ Immunofluorescence: Cortactin Antibody [NBP2-15971] - Cortactin antibody [N1], N-term detects Cortactin protein at cytoplasm and cell membrane by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cortactin stained by Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242014648.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242075994.jpg)
![Western Blot: Cortactin Antibody [NBP2-15971] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (NBP2-15971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15971_rabbit-polyclonal-cortactin-antibody-91020242041243.jpg)