| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60928SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22122SS | |||
| Azide and BSA Free | NBP2-80719 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78984SS | |||
| Azide and BSA Free | NBP2-80695 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1317SS | |||
| Azide and BSA Free | NBP2-80649 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56583SS | |||
| Azide and BSA Free | NBP2-80872 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-56688SS | |||
| BSA Free | NBP2-24710SS | |||
| Azide and BSA Free | NB100-56688 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51841SS | |||
![Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunofluorescence-NB300-139-img0006.jpg "Immunohistochemistry: alpha-Internexin Antibody [NB300-139] - Rat cerebellum section stained with rabbit pAb to alpha-internexin, dilution 1:2,000, in green, and chicken pAb to GFAP, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.")
![Western Blot: alpha-Internexin Antibody [NB300-139] - Whole tissue lysates using rabbit pAb to alpha-internexin, dilution 1:10,000 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha-internexin. The alpha-internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0007.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - The expression of a-internexin (INA) was analyzed by Western blotting. White bars are controls, grey bars are enucleated and black bars are anophthalmic mice. Results from V1 (left), V2M (middle) and V2L (right) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159320) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - IF Confocal analysis of SHSY5Y cells using alpha Internexin antibody (NB300-139, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0005.jpg)
![Western Blot: alpha Internexin Antibody [NB300-139] - Western blot of whole rat spinal cord homogenate stained with RPCA-a-Int, at dilution of 1:20,000. A prominent band running at ~66kDa is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kDa is also seen, apparently resulting from dimerization of alpha-internexin.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Western-Blot-NB300-139-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin CPCA-Peri (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Internexin Antibody [NB300-139] - Polyclonal alpha internexin, NB 300-139 (red) staining neuronal progenitor cells. The green stain shows the fibroblast marker, Plectin (not one of our antibodies).](http://images.novusbio.com/fullsize/alpha-Internexin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-139-img0004.jpg)
![Western Blot: alpha-Internexin Antibody [NB300-139] - Proteins involved in morphogenesis.The expression of a-internexin (INA), collapsing response mediator protein 2 (CRMP2) & 4 (CRMP4), neurofilament-low (NFL) & tubuline-b2 (Tub beta 2) were analyzed by Western blotting. White bars are controls, grey bars are enucleated & black bars are anophthalmic mice. Results from V1 (left), V2M (middle) & V2L (right) are shown for each protein. * P < 0.05 & ** P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27410964), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-139_rabbit-polyclonal-alpha-internexin-antibody-310202415371950.jpg)
![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0004.jpg "Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NB110-60928-img0005.jpg)
![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0003.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0006.jpg "Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Human endometrial stromal cells stained with Endoglin/CD105 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0014.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical staining of endoglin (CD105) in B16F10 murine melanoma tumor model. IHC-P image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0013.jpg)
![Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Western-Blot-NBP2-22122-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunocytochemistry-Immunofluorescence-NBP2-22122-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-3A9-Immunocytochemistry-NBP2-22122-img0007.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-Immunohistochemistry-Paraffin-NBP2-22122-img0005.jpg)
![ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100 ng), Purple: Antigen (10 ng), Green: Antigen (50 ng), Blue: Antigen (100 ng).](http://images.novusbio.com/fullsize/CD105-Antibody-3A9-ELISA-NBP2-22122-img0001.jpg)
![Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0002.jpg "Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: GFAP Antibody [NB100-53809] - (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0001.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Knockdown-Validated-NB110-61576-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or Duox2 and analyzed for Nox4 and Duox1/2 proteins in 2 experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0154157), licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0004.jpg)
![Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 in parental HeLa cells and three clones of NOX4 knockout cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0170327), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DUOX2-Antibody-Western-Blot-NB110-61576-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/DUOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61576-img0003.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0016.jpg "Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Differential effects of the 20(R/S)-Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co-immunofluorescence staining of the aortic root for VSMCs (anti-a-SMA antibody, red) and proliferation marker PCNA (green) and DAPI (blue) Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of HepG2 cells using (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunocytochemistry-Immunofluorescence-NBP2-22120-img0008.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis using alpha-Smooth Muscle Actin Antibody (4A4) against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate. The theoretical molecular weight of the antibody is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Western-Blot-NBP2-22120-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using alpha-Smooth Muscle Actin Antibody (4A4) with DAB staining.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Paraffin-NBP2-22120-img0009.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0013.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Effects of the 20(R/S)-Rg3 stereoisomers on early atherogenesis in diabetic mice. D, F, Representative immunohistochemical a-SMA staining and quantification of the plaque smooth muscle cell content (n = 3). Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/10.1111/jcmm.13601) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-NBP2-22120-img0017.jpg)
![Flow Cytometry: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (4A4) (green) and negative control (purple).](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Flow-Cytometry-NBP2-22120-img0007.jpg)
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Representative immunohistochemical alpha-Smooth Muscle Actin staining of mouse aortic sinus. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-Immunohistochemistry-Frozen-NBP2-22120-img0015.jpg)
![ELISA: alpha-Smooth Muscle Actin Antibody (4A4) [NBP2-22120] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-4A4-ELISA-NBP2-22120-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (4A4) - BSA Free [NBP2-22120] - Differential effects of the 20(R/S)‐Rg3 stereoisomers on proliferation of VSMCs within plaques. A, Co‐immunofluorescence staining of the aortic root for VSMCs (anti‐ alpha ‐SMA antibody, red) & proliferation marker PCNA (green) & a bar graph summarizing the results (n = 3). Scale bar: 20 μm. B, Western blot analysis of cyclin D1, cyclin E & PCNA protein expression within plaques. The bands are quantified by densitometric analysis. Protein expression of cyclin D1 & PCNA was normalized to beta ‐actin, & expression of cyclin E was normalized to GADPH (n = 3, 4, & 4 for cyclin D1, cyclin E & PCNA, respectively). The results are expressed as the mean values ± SEM. *P < .05, **P < .01, ***P < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29566305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-22120_mouse-monoclonal-alpha-smooth-muscle-actin-antibody-4a4-310202415395945.jpg)
![Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Western-Blot-NBP1-78984-img0008.jpg "Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Colocalisation of Dsg3 with E-cadherin-catenin complex. Co-IPs demonstrated that both endogenous and exogenous Dsg3 interacted with the E-cadherin-catenin complex in A431 stable lines (the empty vector control (V) and hDsg3.myc (D3)) as well as in HaCaTs. When pulled down with antibodies against the myc tag or with a mouse Dsg3 Ab 5H10 and blotted for E-cadherin and beta-catenin, both proteins were detected in D3 and, to a lesser extent, in V. Conversely, when pulled down with E-cadherin Ab HECD-1 and blotted for Dsg3 or the myc tag and Pg, these proteins were detected in both V and D3 cells. Note that this result also was confirmed in HaCaTs (HaC) where Dsg3 was expressed endogenously (far right blot). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0014211), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - The cultured primary mouse skin keratinocytes were stained at 1:150. The image shows the cytosolic staining. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-NBP1-78984-img0007.jpg)
![Immunohistochemistry: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 on human skin cancer using NBP1-78984.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunohistochemistry-NBP1-78984-img0005.jpg)
![Western Blot: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Analysis of Desmoglein 3 expression in HaCat cell lysate using NBP1-78984.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Western-Blot-NBP1-78984-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in A431 cells with FITC (green). Nuclei were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-Immunofluorescence-NBP1-78984-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Desmoglein-3 Antibody (5H10) [NBP1-78984] - Desmoglein 3 (5H10) antibody was tested in oral HNSCC cells fixed with 1% formaldehyde permeabilized in 0.1% Triton X-100.](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-5H10-Immunocytochemistry-Immunofluorescence-NBP1-78984-img0006.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.")
![<b>Biological Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody. The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-31488-img0033.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-31488] - Analysis of methanol-fixed HeLa, using CD44 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31488-img0016.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human cervical carcinoma. CD44 stained by CD44 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0036.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human endometrial carcinoma. CD44 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0026.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-31488] - Human pancreatic tumor, using CD44 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-31488-img0035.jpg)
![Immunocytochemistry/ Immunofluorescence: CD44 Antibody [NBP1-31488] - CD44 antibody detects CD44 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: CD44 stained by CD44 antibody (NBP1-31488) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-91020242091445.jpg)
![Western Blot: CD44 Antibody [NBP1-31488] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody (NBP1-31488) diluted at 1:45000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-910202420104431.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Non-transfected (–) and transfected (+) Wild-type (WT) and G CD44 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419405756.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-31488] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-31488_rabbit-polyclonal-cd44-antibody-1610202419414564.jpg)
![Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0003.jpg "Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.")
![Immunohistochemistry-Paraffin: CD44 Antibody (OX-50) [NB600-1317] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaining. This antibody clone specifically stained the membranes of the cells close to the bases of the crypts/villi which are known to actively migrate towards the tip of villi.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Immunohistochemistry-Paraffin-NB600-1317-img0005.jpg)
![Flow Cytometry: CD44 Antibody (OX-50) [NB600-1317] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-OX-50-Flow-Cytometry-NB600-1317-img0002.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Western-Blot-NB100-56583-img0004.jpg "Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.")
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0008.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Immunohistochemistry-Paraffin-NB100-56583-img0003.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0005.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0006.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-2J10D7-Flow-Cytometry-NB100-56583-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of NFkB p105/p50 (2J10D7) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nb100-56583_mouse-monoclonal-nfkb-p105-p50-antibody-2j10d7-imgenex-img-5812a-2210202585646.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0005.jpg "Western Blot: Smad7 Antibody [NBP2-24710] - Effect of mTOR inhibitors on TGF-beta activation of mTOR and Smad pathways. Serum-deprived IPF fibroblasts were treated with or without TGF-beta for 15 minutes in the presence or absence of indicated inhibitors followed by Western blot analysis with an anti-phospho-Smad2 or Smad3 antibody. Expression of total Smad-2, 3, 4 and 7 was analyzed by Western blot. Experiment was done on three lines, which are shown in Figure 1; results were similar between the three lines and results from the IPF fibroblasts isolated from surgical lung biopsy are shown here. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0106155), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Smad7-Antibody-Immunohistochemistry-Paraffin-NBP2-24710-img0004.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0001.jpg)
![Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.](http://images.novusbio.com/fullsize/Smad7-Antibody-Western-Blot-NBP2-24710-img0003.jpg)
![Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Western-Blot-NBP1-51841-img0010.jpg "Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0008.jpg)
![Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunohistochemistry-NBP1-51841-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0007.jpg)