| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-61646SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22123SS | |||
| Azide and BSA Free | NBP2-80852 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24551SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18626-0.02mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81880-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70392 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73935 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg "Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.")
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg)
![Western Blot: MMP-1 Antibody (6A5) [NBP2-22123] - Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MMP-1-Antibody-6A5-Western-Blot-NBP2-22123-img0002.jpg "Western Blot: MMP-1 Antibody (6A5) [NBP2-22123] - Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: MMP-1 Antibody (6A5) [NBP2-22123] - Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/MMP-1-Antibody-6A5-Immunocytochemistry-Immunofluorescence-NBP2-22123-img0004.jpg)
![Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MMP-1-Antibody-6A5-Immunohistochemistry-Paraffin-NBP2-22123-img0005.jpg)
![Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/MMP-1-Antibody-6A5-Flow-Cytometry-NBP2-22123-img0003.jpg)
![ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).](http://images.novusbio.com/fullsize/MMP-1-Antibody-6A5-ELISA-NBP2-22123-img0001.jpg)
![Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0005.jpg "Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Western Blot: c-Maf Antibody [NBP2-24551] - SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4C, overnight. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0006.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0004.jpg)
![Western Blot: c-Maf Antibody [NBP2-24551] - Analysis of Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed at 3 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Western-Blot-NBP2-24551-img0001.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human kidney probed with c-maf antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0002.jpg)
![Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - Staining of Human placenta probed with c-maf antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/c-Maf-Antibody-Immunohistochemistry-Paraffin-NBP2-24551-img0003.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) [NBP1-18626] - (lower a) Internal thoracic artery wall harvested from a 67- year old patient diagnosed with double-vessel CAD showing immunohistochemical staining of VEGF-C. VEGF-C is expressed in smooth muscle cells of the tunica media (TM) and endothelial cells present in the tunica intima (TI). (lower a) Internal thoracic artery wall harvested from a 71-year old patient diagnosed with triple-vessel CAD showing immunohistochemical staining of VEGF-C. Intensive VEGF-C expression is observed in the smooth muscle cells of the tunica media (TM) and in the endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-107-F10-Immunohistochemistry-NBP1-18626-img0004.jpg "Immunohistochemistry: VEGF-C Antibody (107/F10) [NBP1-18626] - (lower a) Internal thoracic artery wall harvested from a 67- year old patient diagnosed with double-vessel CAD showing immunohistochemical staining of VEGF-C. VEGF-C is expressed in smooth muscle cells of the tunica media (TM) and endothelial cells present in the tunica intima (TI). (lower a) Internal thoracic artery wall harvested from a 71-year old patient diagnosed with triple-vessel CAD showing immunohistochemical staining of VEGF-C. Intensive VEGF-C expression is observed in the smooth muscle cells of the tunica media (TM) and in the endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.")
![Western Blot: VEGF-C Antibody (107/F10) [NBP1-18626] - Analysis of recombinant human VEGF-C derived from insect cells using a monoclonal antibody directed against recombinant human/rat VEGF-C.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-107-F10-Western-Blot-NBP1-18626-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: VEGF-C Antibody (107/F10) [NBP1-18626] - Human myofibroblasts stained with VEGF-C antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-107-F10-Immunocytochemistry-Immunofluorescence-NBP1-18626-img0003.jpg)
![Immunohistochemistry-Paraffin: VEGF-C Antibody (107/F10) [NBP1-18626] - Human gastric cancer tissue section stained with VEGF-C antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-107-F10-Immunohistochemistry-Paraffin-NBP1-18626-img0002.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) - Azide and BSA Free [NBP1-18626] - Internal thoracic artery wall harvested from a patient diagnosed with double-vessel CAD showing immunohistochemical staining. Immunohistochemical staining of VEGF-C (a), CAV2 (b) & CAV3 (c) in an internal thoracic artery obtained from a 67-year-old patient who developed the early graft restenosis within 7 months after coronary artery bypass grafting. VEGF-C is expressed in smooth muscle cells of the tunica media (TM) & endothelial cells present in the tunica intima (TI). CAV2 is present exclusively in smooth muscle cells. Strong expression of CAV3 is observed in smooth muscle cells & in endothelial cells. d Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18626_mouse-monoclonal-vegf-c-antibody-107-f10-31020241539259.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) - Azide and BSA Free [NBP1-18626] - Immunohistochemical staining of a saphenous vein wall harvested from a patient diagnosed with triple-vessel CAD. Three serial histological sections of a graft wall obtained from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting (the same patients as mentioned in Fig. 7). VEGF-C (a), VEGFR-3 (b), & CAV3 (c) shown to be expressed by immunohistochemical staining in smooth muscle cells of the tunica media (TM). In addition, VEGFR-3 is strongly present in endothelial cells of the tunica intima (TI). d Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18626_mouse-monoclonal-vegf-c-antibody-107-f10-310202415371967.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) - Azide and BSA Free [NBP1-18626] - Immunohistochemical staining of an internal thoracic artery & saphenous vein wall harvested from a patient with no evidence of luminal stenosis 12 months after CABG. Immunohistochemical staining of VEGF-C (a) & CAV2 (b) in an internal thoracic artery (ITA) graft & VEGFR-3 (c) in saphenous vein (SV) transplant harvested from a 65-year-old patient diagnosed with triple-vessel CAD. Note that lack of CAV2 expression within smooth muscle cells (SMC) in ITA wall is accompanied by VEGF-C positive expression in the same area. In SV tissue sample harvested from the same patient, no VEGRR-3 expression was found. d Negative control. TI tunica intima, TM tunica media, TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18626_mouse-monoclonal-vegf-c-antibody-107-f10-31020241535674.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) - Azide and BSA Free [NBP1-18626] - Saphenous vein wall harvested from a patient diagnosed with double-vessel CAD showing immunohistochemical staining. VEGF-C (a) & VEGFR-3 (b) reactivity in a graft wall obtained from a 67-year-old patient who developed the early graft restenosis within 7 months after coronary artery bypass grafting (the same patient as mentioned in Fig. 6). VEGF-C is localized exclusively within individual smooth muscle cells of the tunica media (TM). Cells with positive VEGFR-3 expression are situated in endothelial cells of the tunica intima (TI). c Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18626_mouse-monoclonal-vegf-c-antibody-107-f10-310202415395931.jpg)
![Immunohistochemistry: VEGF-C Antibody (107/F10) - Azide and BSA Free [NBP1-18626] - Immunohistochemistry of an internal thoracic artery wall harvested from a patient diagnosed with triple-vessel CAD. Immunohistochemical staining of VEGF-C (a), CAV2 (b), & CAV3 (c) in an internal thoracic artery graft harvested from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting. Intensive VEGF-C & CAV3 expression is observed in the smooth muscle cells of the tunica media (TM) & in the endothelial cells of the tunica intima (TI). The presence of immunopositive expression of CAV2 (c) is limited to smooth muscle cells of the tunica media (TM). Expression of all analyzed proteins is present in the small blood vessels localized in the tunica adventitia (TA, arrows). d Negative control Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18626_mouse-monoclonal-vegf-c-antibody-107-f10-310202416165622.jpg)
![Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Western-Blot-H00009700-M01-img0005.jpg "Western Blot: Separase Antibody (6H6) [H00009700-M01] - ESPL1 monoclonal antibody (M01), clone 6H6 Analysis of ESPL1 expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Separase Antibody (6H6) [H00009700-M01] - Analysis of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Separase-Antibody-6H6-Immunohistochemistry-Paraffin-H00009700-M01-img0004.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation and activation. FBS and LPA activate YAP/TAZ and induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels, while NUAK2 and ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean +/- SD (n = 3). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NUAK2 Antibody [NBP1-81880] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81880-img0010.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0015.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - Analysis in control (vector only transfected HEK293T lysate) and NUAK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NUAK2-Antibody-Western-Blot-NBP1-81880-img0011.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0012.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0013.jpg)
![Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Immunohistochemistry-Paraffin-NBP1-81880-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, and target gene expression in MDA-MB231 cells. YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NUAK2-Antibody-Knockdown-Validated-NBP1-81880-img0016.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation & activation. a, b FBS & LPA activate YAP/TAZ & induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels (a), while NUAK2 & ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean ± SD (n = 3). c–h WZ4003 (10 μM) blocks serum/LPA-induced NUAK2 & ANKRD1 mRNA expression. FBS-induced or LPA-induced YAP/TAZ dephosphorylation (e & g), or NUAK2 & ANKRD1 mRNA expression (c, f, h) was monitored at the indicated times (d). Expression data are plotted as the mean ± range for a representative experiment (c, f, h) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241535685.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612198.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416113541.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 is associated with high-grade human bladder cancer. a, c Expression of NUAK2 & the YAP Signature Genes (YAP SG) is enhanced both in high-grade non-muscle-invasive (HG-NMIBC; n = 13, **p = 0.0071) & muscle-invasive (pT2 MIBC; n = 9, **p = 0.0033) bladder cancers39 as compared to LG-NMIBC (n = 27) using an unpaired t test. b Elevated expression of NUAK2 is correlated with disease recurrence in a cohort41 of MIBC patient samples with n = 57 disease free & n = 56, recurred samples (*p = 0.0172). Box & whisker plots show the median (line in the box), first & third quartiles (lower & upper ends of the box), & the minimum & maximum values (whiskers in the plot). Dots represent a single patient sample. d A heat map depicting expression of NUAK2 & YAP SG in NMIBC LG (open circle), HG (closed circle), & MIBC pT2 (slashed circle) bladder cancer samples39. e Characterization of high-grade-derived & low-grade-derived bladder cancer cell lines. Scale bar, 25 μm. f NUAK2 mRNA expression is elevated in HG-derived (TCCSUP & T24) BC cell lines as compared to LG lines. Data are plotted as the mean ± SD (n = 3). g–i Blocking NUAK2 activity with 10 μM WZ4003 (g) or NUAK2 expression in stable clones (#5 or #7) using inducible NUAK2 shRNAs (h, i) inhibits the growth of HG BC cell lines as measured by SRB (g) or DAPI (h) staining. Data are plotted as the mean ± SD of a representative experiment, n = 3 (for TCC) or mean ± range of two experiments (for T24) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-310202416114727.jpg)
![Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81880_rabbit-polyclonal-nuak2-antibody-31020241612229.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0008.jpg "Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0007.jpg)
![Immunohistochemistry-Paraffin: Cdk2 Antibody (2D9) [NBP2-00776] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunohistochemistry-Paraffin-NBP2-00776-img0006.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0003.jpg)
![Western Blot: Cdk2 Antibody (2D9) [NBP2-00776] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cdk2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cdk2.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Western-Blot-NBP2-00776-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk2 Antibody (2D9) [NBP2-00776] - Immunofluorescent staining of HeLa cells using anti-Cdk2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NBP2-00776-img0005.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Hela cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0001.jpg)
![Flow Cytometry: Cdk2 Antibody (2D9) [NBP2-00776] - Analysis of Jurkat cells, using anti-Cdk2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Cdk2-Antibody-2D9-Flow-Cytometry-NBP2-00776-img0002.jpg)
![CDK2 Antibody (OTI2D9)[NBP2-00776]Simple Western� analysis of endogenous protein TUBB4A from HELA lysates (0.2 mg/mL) using TUBB4A Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-00776_mouse-cdk2-mab-oti2d9-simple-western-131120251236227.jpg%253fstore=america&image-type=image)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0021.jpg "Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 and IMR90 cells. IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, and the pre-immune serum (PI) used as a control for the immunoprecipitation. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fgene.2013.00095/abstract), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunocytochemistry-Immunofluorescence-NBP2-02182-img0018.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human thyroid tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0017.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0002.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0003.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Western-Blot-NBP2-02182-img0019.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0005.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0011.jpg)
![Immunohistochemistry-Paraffin: RPA2 Antibody (OTI9A1) [NBP2-02182] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunohistochemistry-Paraffin-NBP2-02182-img0016.jpg)
![Flow Cytometry: RPA2 Antibody (OTI9A1) [NBP2-02182] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Flow-Cytometry-NBP2-02182-img0001.jpg)
![Immunoprecipitation: RPA2 Antibody (OTI9A1) [NBP2-02182] - (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/RPA2-Antibody-OTI9A1-Immunoprecipitation-NBP2-02182-img0020.jpg)
![Western Blot: RPA2 Antibody (OTI9A1) [NBP2-02182] - Ajuba associates with RPA in HTC75 & IMR90 cells. (A) IP-Western probed with a total anti-RPA32 antibody after immunoprecipitation with a commercial anti-Ajuba antibody (com.) or anti-peptide serum from IMR90 or HTC75 extracts as indicated. Left panel: total lysates (input) for each cell line, & the pre-immune serum (PI) used as a control for the immunoprecipitation. (B) Western for RPA32-p-Thr21 in both HTC75 & IMR90Ajuba-depleted cells. A non-specific band is marked by a *. Total RPA32 levels are shown at the bottom. (C) Model for the role of Ajuba in repression of ATR. The “?” indicates that the interaction between Ajuba & RPA could be direct or indirect. See text for details. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23755068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-02182_mouse-rpa2-mab-oti9a1-310202415291913.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)