| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47785-0.1mg | |||
| IHC-Prediluted | NBP2-48331 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27402 | |||
| BSA Free | NB100-56748SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85500-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13732-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77253-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43359-0.05mg | |||
| BSA Free | NBP1-43359-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76963-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70135 | |||
![Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NB100-562-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0010.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-562-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and NB100-565 were used.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0004.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Western-Blot-NBP2-44342-img0003.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Formalin-fixed, paraffin-embedded Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Immunohistochemistry-Paraffin-NBP2-44342-img0002.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Colon Carcinoma stained with CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Immunohistochemistry-Paraffin-NBP2-44342-img0001.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Cholangiocellular carcinoma (liver cancer tissue) stained with ATTO550 conjugated CD31 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-44342_mouse-monoclonal-cd31-pecam-1-antibody-c31-3-c31-7-c31-10-17720241044653.jpg)
![Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Western-Blot-NB100-56748-img0002.jpg "Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml."){kind=icon}
![Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Immunohistochemistry-Paraffin-NB100-56748-img0010.jpg){kind=icon}
![Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Flow-Cytometry-NB100-56748-img0006.jpg){kind=icon}
![Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Immunohistochemistry-Frozen-NB100-56748-img0011.jpg){kind=icon}
![Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Flow-Cytometry-NB100-56748-img0005.jpg){kind=icon}
![Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.](http://images.novusbio.com/fullsize/RhoA-Antibody-Western-Blot-NB100-91273-img0034.jpg "Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.")
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0024.jpg)
![Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-NB100-91273-img0040.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0023.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0029.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0031.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0032.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0016.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on mouse brain (cryo section) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0035.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining in human skin and skeletal muscle tissues using anti-PTGS1 antibody. Corresponding PTGS1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: COX-1 Antibody [NBP1-85500] - Analysis in human cell line HELA.](http://images.novusbio.com/fullsize/COX-1-Antibody-Western-Blot-NBP1-85500-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: COX-1 Antibody [NBP1-85500] - Staining of human cell line BJ shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85500-img0009.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0010.jpg)
![Immunohistochemistry-Paraffin: COX-1 Antibody [NBP1-85500] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/COX-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85500-img0011.jpg)
![Simple Western: COX-1 Antibody [NBP1-85500] - Lane view shows one specific signal (~70kDa) in lysates prepared from the mouse tail vein. Image from a verified customer review.](http://images.novusbio.com/fullsize/COX-1-Antibody-Simple-Western-NBP1-85500-img0013.jpg)
![Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PARP-Antibody-Western-Blot-NBP2-13732-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PARP Antibody [NBP2-13732] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PARP Antibody [NBP2-13732] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13732-img0007.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0014.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human lymph node shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0010.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human pancreas shows strong nuclear positivity.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0011.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human parathyroid gland shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0012.jpg)
![Immunohistochemistry-Paraffin: PARP Antibody [NBP2-13732] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PARP-Antibody-Immunohistochemistry-Paraffin-NBP2-13732-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414593.jpg "Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![<b>Genetic Strategies Validation. </b>Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - KO Validation in 293T Cells. Loading: 10 u of lysate Antibodies: XBP-1, 4 u/mL and beta-actin, 1u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202419313294.gif)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of lysates per lane. Antibodies: XBP-1 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000dilution. (1) the absence and (2) the p](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0021.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of HepG2 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane1: 1 ug/mL Lane2: 2 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0018.jpg)
![Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 15 ug of A549 cell lysate Antibodies: XBP-1, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goatanti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1:0.25 ug/mL Lane2: 0.5 ug/mL Lane3: 1 ug/mL](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0019.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: XBP1 Antibody [NBP1-77253] - Loading: 100 ng of human XBP-1 recombinant protein perlane. Antibodies: XBP-1 (A: 0.5 ug/mL, B: 1 ug/mL andC: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77253-img0020.jpg)
![Western Blot: XBP1 Antibody - BSA Free [NBP1-77253] - Validation in Mouse Pancreas Tissue.Loading: 15 u of lysates per lane.Antibodies: XBP-1, (4 u /mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241918647.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Mouse Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241956210.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Liver Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420312941.gif)
![Immunocytochemistry/ Immunofluorescence: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP-1 in Human Pancreas Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120242013185.gif)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420441134.jpg)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Human Liver Tissue.Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-2711202420414538.jpg)
![Immunohistochemistry: XBP1 Antibody - BSA Free [NBP1-77253] - Validation of XBP1 in Rat Spleen Tissue.Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP1 antibody at 1 u /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77253_rabbit-polyclonal-xbp1-antibody-271120241923693.jpg)
![Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg "Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.")
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Independent Antibodies Validation. </b>Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in HeLa Cells. Loading: 15 ug of lysate per lane. Antibodies: Bim NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate. Bim antibody can detect all three human isoforms, including EL, L and S isoforms.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0009.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Human Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP1-76963-img0010.jpg)
![Western Blot: Bim Antibody - BSA Free [NBP1-76963] - Figure 3 Western Blot Validation in Human TissueLoading: 15 u of lysates per lane.Antibodies: BIM , (0.5 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-2711202420115554.gif)
![Immunocytochemistry/ Immunofluorescence: Bim Antibody - BSA Free [NBP1-76963] - Validation of BIM in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76963_rabbit-polyclonal-bim-antibody-271120242045646.gif)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0009.jpg "Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.")
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB detection of AKT1 in human MD-MB-231 cells using AKT1 antibody NBP2-01724 at a dilution of 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (OTI4D6) [NBP2-01724] - ICC/IF of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-01724-img0008.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (10 ug) from a mouse cell line and 3 different mouse tissues using AKT1 antibody at a 1:200 dilution.](http://images.novusbio.com/fullsize/Akt1-Antibody-4D6-Western-Blot-NBP2-01724-img0012.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human prostate tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0007.jpg)
![Flow Cytometry: AKT1 Antibody (OTI4D6) [NBP2-01724] - Flow cytometric analysis of Jurkat cells using anti-AKT1 antibody (red), compared to a nonspecific negative control antibody (blue).](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Flow-Cytometry-NBP2-01724-img0001.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY AKT1 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0002.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded adenocarcinoma of human endometrium tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0003.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human bladder tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human kidney tissue using AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human kidney tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0006.jpg)
![AKT1 Antibody (OTI4D6)[NBP2-01724]Simple Western� analysis of endogenous protein BCAT1 from HepG2 lysates (0.5 mg/mL) using BCAT1 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:20 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:20 dilutios of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-01724_mouse-akt1-mab-oti4d6-simple-western-131120251236921.jpg%253fstore=america&image-type=image)
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB100-1194-img0007.jpg "Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145588.jpg)
![Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-1311202311455816.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145587.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145584.jpg)
![Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-NB100-2059-img0015.jpg "Immunohistochemistry: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Representative photomicrographs showing Thrombospondin-1 (A) immunostaining in duodenal sections of 1 non-CD controls (NC) and 1 active celiac disease (ACD) patient. Isotype control staining is also shown. Right panels: positive cells were counted in at least 5 fields/section of duodenal samples of 5 NC and 5 ACD patients and expressed as mean +/- SD of all experiments. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0100980), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Staining in human placenta.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the skin of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Ang II increases the number of TSP-1 positive cells in mouse heart and skin.IHC staining of TSP-1 was performed on paraffin sections from the heart of PBS and Ang II treated WT mice. Representative photographs are shown from five animals per group. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Thrombospondin-1-Antibody-A6-1-Immunohistochemistry-Paraffin-NB100-2059-img0014.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody (A6.1) [NB100-2059] - Reduced levels of vascular & pericyte markers in MMP12KO mice.A. IHC staining of vWF, TSP-1 & PDGFR beta was performed on paraffin sections from the heart of PBS & Ang II treated WT & MMP12KO mice. B. IHC staining of vWF, & PDGFR beta was performed on paraffin sections from the skin of PBS & Ang II treated WT & MMP12KO mice. Representative photographs are shown from three animals per group. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2059_mouse-monoclonal-thrombospondin-1-antibody-a6-1-310202415304261.jpg)