| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24722SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33157 | |||
| IHC-Prediluted | NBP2-44428 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56146SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-42822SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-207SS | |||
![Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Western-Blot-NBP2-24722-img0002.jpg "Western Blot: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of human adrenal using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-24722-img0003.jpg)
![Immunohistochemistry-Paraffin: CYP1B1 Antibody [NBP2-24722] - Analysis of CYP1B1 in human brain tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/CYP1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-24722-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0010.jpg "Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cdk4-Antibody-Immunohistochemistry-Paraffin-NBP1-31308-img0006.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CDK4 Antibody [NBP1-31308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDK4-Antibody-Western-Blot-NBP1-31308-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CDK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31308-img0009.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024205643.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-9102024209146.jpg)
![Western Blot: CDK4 Antibody [NBP1-31308] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (NBP1-31308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31308_rabbit-polyclonal-cdk4-antibody-910202420104465.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0003.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.")
![Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Western-Blot-NBP2-25221-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (CCP58).](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0001.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0006.jpg "Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-325] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-325-img0003.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Whole cell lysate (50 ug) from HeLa (H) and 293T (50 ug) probed with GAPDH Antibody diluted at 0.04 ug/ml](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0001.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Another Affinity purified rabbit anti-GAPDH used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0005.jpg)
![Simple Western: GAPDH Antibody [NB300-325] - Simple Western lane view shows a specific band for GAPDH in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-325-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212345.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212378.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416205140.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-31020241621812.jpg)
![Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56165-img0003.jpg "Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,")
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of paraffin-embedded human lung carcinoma using this antibody at dilution of 1:25. Image from verified customer review.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NB100-56165-img0004.jpg)
![Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of MCF7 (A) and K562 (B) cell lysate using this antibody at 1:500.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56165-img0002.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using this antibody at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NB100-56165-img0001.jpg)
![Simple Western: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Shows a specific band for Smac in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Simple-Western-NB100-56165-img0005.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0009.jpg "Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).")
![Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDK-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2383-img0007.jpg)
![Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.](http://images.novusbio.com/fullsize/PDK-1-Antibody-Western-Blot-NB100-2383-img0008.jpg)
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - Expression of BRIP1/FANCJ at the mRNA (graphs) and protein (photos) levels in iPSCs. Gene expression was assessed with RNA-seq in FACS-sorted bone marrow HSCs or with real-time RT-PCR in cultured iPSCs. The box-and-whiskers graphs show the median, 25th and 75th percentile (box) minimum, and maximum values (whiskers) of the FPKM (fragments per kilobase million) or relative expression in comparison to house-keeping genes (geometric mean for HPRT, B2M, and beta-actin), n = 4, * p Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.Green: BRIP1 protein stained by BRIP1/FANCJ antibody [N1N2], N-term (NBP1-31883) diluted at 1:500.Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/nbp1-31883_rabbit-polyclonal-brip1-fancj-antibody-immunocytochemistry-immunofluorescence-141120251843320.jpg)
![Western Blot: MCL1 Antibody [NB100-56146] - analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1:2000.](http://images.novusbio.com/fullsize/MCL1-Antibody-Western-Blot-NB100-56146-img0002.jpg "Western Blot: MCL1 Antibody [NB100-56146] - analysis of Mcl-1 in A) Ramos and B) ThP-1 lysate using Mcl-1 antibody at 1:2000.")
![Immunohistochemistry-Paraffin: MCL1 Antibody [NB100-56146] - Formalin-fixed paraffin-embedded tissue sections of human diffuse, aggressive Non-Hodgkin's lymphoma (NHL) of B cell lineage stained for Mcl-1 expression using Mcl-1 antibody at 1:2000. A-D, Mcl-1 staining shown as varying magnifications. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/MCL1-Antibody-Immunohistochemistry-Paraffin-NB100-56146-img0003.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (812) [NBP2-43560] - Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (812) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Frozen sectioned E13.5 Rat brain. Green: Vimentin protein stained by Vimentin antibody [812] diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0006.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody (812) [NBP2-43560] - Analysis of Frozen section of embryonic mouse brain (mE18.5). Red: Vimentin antibody [812] diluted at 1:250. Blue: DAPI.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Immunohistochemistry-Frozen-NBP2-43560-img0001.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract 10 % SDS-PAGE Vimentin antibody [812] dilution: 1:1000](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Western-Blot-NBP2-43560-img0002.jpg)
![Sandwich ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using Vimentin Antibody (812) as capture antibody at concentration of 5 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Vimentin-Antibody-812-Sandwich-ELISA-NBP2-43560-img0011.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Non-transfected (-) and transfected (+) 293T whole cell extracts (10 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131230.jpg)
![ELISA: Vimentin Antibody (812) [NBP2-43560] - Sandwich ELISA detection of recombinant full-length Vimentin protein using NBP2-43560 as capture antibody at concentration of 5 ug/mL and detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-91020242041256.jpg)
![Western Blot: Vimentin Antibody (812) [NBP2-43560] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody (NBP2-43560) diluted at 1:500000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-910202420131251.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:3000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202519369.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-2611202518434512.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:5000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251820010.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (NBP2-43560) diluted at 1:75000.](http://images.novusbio.com/fullsize/nbp2-43560_mouse-monoclonal-vimentin-antibody-812-western-blot-261120251837248.jpg)
![Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Western-Blot-NBP1-41185-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.")
![<b>Genetic Strategies Validation. </b>Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Chromatin-Immunoprecipitation-NBP1-41185-img0009.jpg)
![Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Western-Blot-NBP1-41185-img0012.jpg)
![Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.](http://images.novusbio.com/fullsize/BRCA1-Antibody-17F8-Immunoprecipitation-NBP1-41185-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-41185_mouse-monoclonal-brca1-antibody-17f8-1610202419414553.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout (KO) HeLa cell line. PVDF membrane was probed with Rabbit Anti-Human p62/SQSTM1 polyclonal Antibody (Catalog # NBP1-42822) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Knockout-Validated-NBP1-42822-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout (KO) HeLa cell line. PVDF membrane was probed with Rabbit Anti-Human p62/SQSTM1 polyclonal Antibody (Catalog # NBP1-42822) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42822-img0008.jpg)
![Simple Western: p62/SQSTM1 Antibody [NBP1-42822] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Simple-Western-NBP1-42822-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - Hyperosmotic stress stimulatesA utophagyHeLa and HCT 116 cells were transduced with an adenovirus coding for GFP-LC3 (Ad GFP-LC3) for 24 h. LC3 I-to-LC3 II conversion and p62/SQSTM1 depletion were assessed by Western blot analysis in HeLa and HCT116 cells treated with various concentrations of sorbitol or mannitol (25-300 mOsm) .It indicated times (0.5-6 h). Image collected and cropped by Citeab from the following publication ( licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42822-img0010.jpg)
![Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Western-Blot-NB500-207-img0001.jpg "Western Blot: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Analysis of CAF1 p150 expression in Ntera2 whole cell lysate using NB500-207.")
![Immunocytochemistry/Immunofluorescence: CHAF1A Antibody (SS 1 1-13) [NB500-207] - Immunofluorescent staining of CAF-1-p150 in HeLa cells (Shibahara et al 1999).](http://images.novusbio.com/fullsize/CHAF1A-Antibody-SS-1-1-13-Immunocytochemistry-Immunofluorescence-NB500-207-img0004.jpg)