| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-164SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-147SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36561SS | |||
| Azide and BSA Free | NBP2-80818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60928SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56104SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87069-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77452SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76985-0.025mg | |||
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Western blot analysis of lipid transporters in macrophages treated with or without oxLDL using antibodies against ABCA1 (NB400-164), SR-B1 (NB400-104), and CD36 (NB400-144) . Lrp1Y63F impairs Abca1-mediated cholesterol efflux and increases lipid accumulation in macrophages. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29292) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of FFPE mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Immunohistochemistry-Paraffin-NB400-164-img0007.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB400-164 at various dilutions in ABCA1 transfected HeLa lysates.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of ABCA1 in (A) T09 treated RAW 264.7 cells and (B) untreated RAW 264.7 cells.](http://images.novusbio.com/fullsize/ABCA1-Antibody-3A1-891-3-Western-Blot-NB400-164-img0006.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation downregulates hepatic Abca1 levels.(A) Western blot analysis of Abca1 expression in the liver from Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with HCHF diet for 16 weeks (n = 5, each group). (B) Western blot analysis of Abca1 expression in the liver from 16 week HCHF-fed Lrp1Y63F;Ldlr−/− & Ldlr−/− mice after BMT (n = 5, each group). All data are mean ±SEM. **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241535616.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416175236.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F impairs Abca1 induction through inhibiting the PPAR gamma /LXR pathway.(A) Real-time PCR analysis of Abca1, Abca7, Pparg, Nr1h3 & Nr2h2 expression in macrophages treated with or without ox-LDL. (B–E) Isolated peritoneal macrophages from Lrp1Y63F;Ldlr−/− & Ldlr−/− were pre-incubated for 1 hr with (B) T0070907 (PPAR gamma antagonist, 10 μM), (C) Rosiglitizone (PPAR gamma agonist) at indicated concentrations in μM, (D) T0901317 (nonspecific LXR agonist) at indicated concentrations in μM, (E) LXR623 (LXR beta full agonist & LXR alpha partial agonist) at indicated concentrations in μM, followed by the treatment with or without oxLDL for an additional 24 hr. Abca1 expression was assessed by immunoblot analysis (left). Protein expression was quantified & analyzed (right). Data are mean ±SEM from three independent experiments. *p<0.05; **p<0.01. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-310202416154639.jpg)
![Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Lrp1Y63F mutation has no effect on atherogenic signaling pathways & morphological phenotype in SMCs.(A) Left: representative images of H&E stained descending thoracic aortas (scale bar = 50 μm). Right: aortic wall thickness & cell number were quantified for the indicated genotypes. Data are expressed mean ±SEM; n = 3 per group & genotype. (B) HCHF diet in Lrp1Y63F;Ldlr−/− mice has no effect on PDGF & TGF beta -mediated signaling in the aorta. Proteins were extracted from whole aorta in Lrp1Y63F;Ldlr−/− & Ldlr−/− mice fed with indicated diets. Expression of proteins involved in PDGF signaling pathways was determined by Western blot analysis. (C) Lrp1Y63F mutation did not affect atherogenic signaling pathways mediated by PDGF in vitro. Primary SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB for 10 min. Expression of proteins involved in PDGF signaling were determined by Western blot analysis. (D) Lrp1Y63F mutation did not alter SMC functions in vitro. Primary cultured SMCs from WT & Lrp1Y63F mice were incubated with or without 10 ng/ml PDGF-BB under indicated conditions, then cell migration (left) & proliferation (right) were determined. Values from three independent experiments are expressed as mean ±SEM. *p<0.05. Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/29292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-164_rat-monoclonal-abca1-antibody-3a1-891-3-31020241616063.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0007.jpg)
![Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0006.jpg "Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.")
![Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-106-img0002.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression and activity in RA and OA joints and 3OH-myristate levels in synovial fluid of RA and OA patients. Immunohistological analysis of PLTP expression in synovial tissue from 5 RA and 6 OA patients stained for PLTP and macrophages (CD68+ cells); All RA synovial tissue showed positive stainings while only 2 out 6 OA tissues were positive for PLTP staining. Representative stainings are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0008.jpg)
![Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0001.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP antibody expression in RA synovial tissues. (A) Immuno-histological analyses used RA patients synovial tissue sections (n=5). They were stained for PLTP or macrophages (CD68+ cells). Blue arrows indicate macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with CD68+ staining (black arrow; non-exhaustive) and morphological features. (B, C) Double staining displays localization (n = 3) of PLTP with macrophages (CD68+) in B or RA-FLS (alpha-SMA+) in C. Overlay is shown to visualize co-localization of PLTP. All magnification and fluorescence analysis at 20x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29565987/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0005.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-Paraffin-NB400-106-img0007.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-106_rabbit-polyclonal-pltp-antibody-310202416171467.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - An intracellular stain was performed on A431 cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0005.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0003.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-P4C10-Flow-Cytometry-NBP2-36561-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Overnight incubation with 1:50 Integrin beta 1/CD29 primary antibody in PBS/BSA 1%, at 4ºC. Green: Integrin beta 1/CD29 Antibody (P4C10), Red: Phalloidin, Blue: DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-36561_mouse-monoclonal-integrin-beta-1-cd29-antibody-p4c10-immunocytochemistry-immunofluorescence-1462023134549..jpg)
![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0004.jpg "Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.")
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NB110-60928-img0005.jpg)
![Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Western-Blot-NB110-60928-img0003.jpg)
![Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0002.jpg "Western Blot: GFAP Antibody [NB100-53809] - (0.005ug/ml) staining of Human Cerebellum (A) and (0.01ug/ml) Cerebral Cortex (B) (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: GFAP Antibody [NB100-53809] - (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/GFAP-Antibody-Western-Blot-NB100-53809-img0001.jpg)
![Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-Western-Blot-NB100-56104-img0007.jpg "Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. Recombinant Bcl-XL (positive control).")
![Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bcl-xL-Antibody-Western-Blot-NB100-56104-img0008.jpg)
![Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-xL expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-xL expression.](http://images.novusbio.com/fullsize/Bcl-Xl-Antibody-Immunohistochemistry-Paraffin-NB100-56104-img0006.jpg)
![Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-xL at ~30 kDa.](http://images.novusbio.com/fullsize/Bcl-Xl-Antibody-Western-Blot-NB100-56104-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in human breast carcinoma at 1:2000. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An organellar/mitochondrial pattern of Bcl-xL staining is observed in the cytosol of tumor cells.](http://images.novusbio.com/fullsize/Bcl-Xl-Antibody-Immunohistochemistry-Paraffin-NB100-56104-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SDHB Antibody [NBP1-87069] - Staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87069-img0015.jpg "Immunocytochemistry/Immunofluorescence: SDHB Antibody [NBP1-87069] - Staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & mitochondria. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunohistochemistry-Paraffin-NBP1-87069-img0008.jpg)
![Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunohistochemistry-Paraffin-NBP1-87069-img0009.jpg)
![Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human duodenum shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SDHB-Antibody-Immunohistochemistry-Paraffin-NBP1-87069-img0010.jpg)
![Simple Western: SDHB Antibody [NBP1-87069] - Simple Western lane view shows a specific band for SDHB in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SDHB-Antibody-Simple-Western-NBP1-87069-img0005.jpg)
![Simple Western: SDHB Antibody [NBP1-87069] - Electropherogram image(s) of corresponding Simple Western lane view. SDHB antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/SDHB-Antibody-Simple-Western-NBP1-87069-img0006.jpg)
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NB600-252-img0012.jpg "Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RbBP5-Antibody-Immunohistochemistry-NB600-252-img0008.jpg)
![Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB600-252-img0009.jpg)
![Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Western-Blot-NB600-252-img0011.jpg)
![Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-252 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/RbBP5-Antibody-Chromatin-Immunoprecipitation-NB600-252-img0007.jpg)
![Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution.](http://images.novusbio.com/fullsize/TRAILR4-TNFRSF10D-DcR2-Antibody-Western-Blot-NBP1-76985-img0001.jpg "Western Blot: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-DcR2 at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody [NBP1-76985] - Staining of HeLa cells using DcR2 antibody at 10ug/ml.](http://images.novusbio.com/fullsize/TRAILR4-TNFRSF10D-DcR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76985-img0002.jpg)
![Immunohistochemistry: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunohistochemistry of TRAILR4/TNFRSF10D/DcR2 in mouse kidney node tissue with TRAILR4/TNFRSF10D/DcR2 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-76985_rabbit-polyclonal-trailr4-tnfrsf10d-dcr2-antibody-27112024202292.gif)
![Immunocytochemistry/ Immunofluorescence: TRAILR4/TNFRSF10D/DcR2 Antibody - BSA Free [NBP1-76985] - Immunofluorescence of TRAILR4/TNFRSF10D/DcR2 in mouse kidney tissue withTRAILR4/TNFRSF10D/DcR2 antibody at 20 ug/ml.Green: TRAILR4/TNFRSF10D/DcR2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76985_rabbit-polyclonal-trailr4-tnfrsf10d-dcr2-antibody-2711202419592062.gif)