| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-103SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07047SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-32954-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80289-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2068SS | |||
| Azide and BSA Free | NBP2-80554 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-42821SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37364SS | |||
![Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Knockdown-Validated-NB600-103-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: Calreticulin Antibody [NB600-103] - Lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Simple-Western-NB600-103-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-103-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - Analysis of FFPE human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in glands was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunohistochemistry-Paraffin-NB600-103-img0007.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB600-103 at 1:1,000. ECL exposure, 10 seconds.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0003.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0004.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0002.jpg "Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.")
![Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0004.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Genetic manipulations can alter PDH E1 alpha phosphorylation in response to hypoxia.(a) MIA PaCa-2 control (Hg), silenced HIF1 alpha (shHIF1 alpha ) or silenced PDHK1 (shPDHK1) & PDP1 overexpressing cells were incubated for 16 h in normoxia or hypoxia (0.5% O2) & phospho-serine E1 alpha detected by Western blot showing that all three modifications can have an inhibitory effect on hypoxic Ser232 phosphorylation. (b) Increased pSer232-E1 alpha detection after E1 alpha immunoprecipitation from lysates of cells treated as in (a). (c) The same analysis as in (a) after treatment with 16 h 1 mM DMOG. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-31020241534333.jpg)
![Western Blot: PDK4 Antibody - BSA Free [NBP1-07047] - Hypoxia inhibits mitochondrial OCR & PDH activity & induces PDHK1 protein & activity.(a) Ratio of oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) measured by Seahorse XF in MIA PaCa-2, PANC-1, & SU.86.86 cell lines in high (25 mM) or low (0.5 mM) glucose incubated overnight with or without 1 mM DMOG. (mean ± SEM, two-tailed Student’s t-test, **p < 0.01, ***p < 0.001) (b) Cell-based PDH activity assay in cells incubated 16 h in normoxia, hypoxia (0.5% O2) or 1 mM DMOG. (mean ± SD, one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001) (c) Western blots of HIF alpha isoforms, pyruvate dehydrogenase kinase isoforms (PDHKs), phosphatase (PDP1 – lower band *), target phosphorylated serine residues on E1 alpha & total E1 alpha after overnight incubation in normoxia or hypoxia (0.5% O2) at 25 or 5 mM glucose as indicated. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27498883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-07047_rabbit-polyclonal-pdk4-antibody-310202416171443.jpg)
![Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Immunohistochemistry-Paraffin-NBP1-32954-img0045.jpg "Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Western-Blot-NBP1-32954-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody ) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32954-img0033.jpg)
![Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human esophageal carcinoma.LOXL2 stained by LOXL2 antibody diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-Immunohistochemistry-Paraffin-NBP1-32954-img0042.jpg)
![ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/Lysyl-Oxidase-Homolog-2-LOXL2-Antibody-ELISA-NBP1-32954-img0044.jpg)
![Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32954_rabbit-polyclonal-lysyl-oxidase-homolog-2-loxl2-antibody-910202419593594.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Lysyl Oxidase Homolog 2/LOXL2 antibody detects Lysyl Oxidase Homolog 2/LOXL2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Lysyl Oxidase Homolog 2/LOXL2 stained by Lysyl Oxidase Homolog 2/LOXL2 antibody (NBP1-32954) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32954_rabbit-polyclonal-lysyl-oxidase-homolog-2-loxl2-antibody-910202420131255.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32954_rabbit-polyclonal-lysyl-oxidase-homolog-2-loxl2-antibody-1610202419405749.jpg)
![Western Blot: TGF-beta 1 Antibody [NBP1-80289] - Sample Tissue: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-80289-img0013.jpg "Western Blot: TGF-beta 1 Antibody [NBP1-80289] - Sample Tissue: Human Fetal Heart. Antibody Dilution: 1.0ug/ml")
![Immunocytochemistry/Immunofluorescence: TGF-beta 1 Antibody [NBP1-80289] - Mouse kidney (red fluorescence). Nuclei were stained with DAPI (blue fluorescence). Working dilutions: 5-10 ug/ml](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80289-img0008.jpg)
![Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-80289] - Human Appendix FFPE tissue section stained with TGF-beta 1 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-80289-img0011.jpg)
![Western Blot: TGF-beta 1 Antibody [NBP1-80289] - Antibody Titration: 0.2-1 ug/ml or 1:5000 to 1:1000 Dilution. SP2/0 cell lysate.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-80289-img0010.jpg)
![Western Blot: TGF-beta 1 Antibody [NBP1-80289] - Sample Tissue: Mouse Spleen. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-80289-img0012.jpg)
![Western Blot: TGF-beta 1 Antibody [NBP1-80289] - Sample Tissue: Mouse Pancreas. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Western-Blot-NBP1-80289-img0014.jpg)
![Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-80289] - Human Spleen Tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-80289-img0007.jpg)
![Immunohistochemistry-Paraffin: TGF-beta 1 Antibody [NBP1-80289] - Human prostate cancer tissue was detected using RP/AEC red color stain. Working dilutions: 5-10 ug/ml.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-80289-img0009.jpg)
![Imaging Mass Cytometry: TGF-beta 1 Antibody [NBP1-80289] - Human bone marrow FFPE tissue sections. DNA in blue, TGF-beta 1 antibody staining in white. IMC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TGF-beta-1-Antibody-Imaging-Mass-Cytometry-NBP1-80289-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal TGF-beta 1 Antibody [NBP1-80289] - Mice hepatocytes stained with TGF-beta 1 Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-80289_rabbit-polyclonal-tgf-beta-1-antibody-immunocytochemistry-immunofluorescence-25112024112535..png)
![Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Western-Blot-NB100-268-img0001.jpg "Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunoprecipitation-NB100-268-img0003.jpg)
![Western Blot: ABCA1 Antibody (HJ1) [NB100-2068] - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue.](http://images.novusbio.com/fullsize/ABCA1-Antibody-HJ1-Western-Blot-NB100-2068-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCA1 Antibody (HJ1) [NB100-2068] - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ABCA1 Antibody (HJ1) [NB100-2068] - Analysis of ABCA1 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ABCA1-Antibody-HJ1-Immunohistochemistry-NB100-2068-img0006.jpg)
![Flow Cytometry: ABCA1 Antibody (HJ1) [NB100-2068] - Staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ABCA1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/ABCA1-Antibody-HJ1-Flow-Cytometry-NB100-2068-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Western-Blot-NBP1-42821-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42821-img0006.jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate from primary rat hepatocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-672023145728..jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate from mice hepatocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-672023145955..jpg)
![Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - Western Blot of the lysate of HepG2 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-western-blot-67202315152..jpg)
![Immunocytochemistry/Immunofluorescence: Rabbit Polyclonal p62/SQSTM1 Antibody [NBP1-42821] - Mouse hepatocytes stained with p62/SQSTM1 Antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-42821_rabbit-polyclonal-p62-sqstm1-antibody-immunocytochemistry-immunofluorescence-25112024113332..png)
![Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0015.jpg "<b>Biological Strategies Validation. </b>Simple Western: GLP-1R Antibody [NLS1205] - Capillary western blot and fold change in the expression of GLP-1 receptors (GLP-1R) in the rat retina. *P < .05, significantly different from CSF group; #P < .05 significantly different from noradrenaline without exendin-4 group. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry: GLP-1R Antibody [NLS1205] - Expression of GLP-1 receptors in human retinal microvascular endothelial cells (HRMECs). (i) Endothelial cells labelled with endothelin-1 (ET-1) antibodies (green). (j) GLP-1 receptors are labelled in red. (k) DAPI staining indicates the cell nucleus (blue). (l) Merged images. (m-p) Evaluation of GLP-1 receptors expression in human retinal pericytes (HRPs). (m) Pericytes labelled with alpha-SMA antibodies (green). (n) GLP-1 receptors are labelled in red. (o) DAPI staining indicates the cell nucleus (blue). (l and p) merged images. Image collected and cropped by CiteAb from the following publication (//bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15059) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunocytochemistry-NLS1205-img0014.jpg)
![Western Blot: GLP-1R Antibody [NLS1205] - WB detection of GLP-1R in lysates of human (1) pancreas (2) lung and (3) kidney tissues using 2 ug/mL concentration of GLP-1R antibody.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Western-Blot-NLS1205-img0012.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Analysis of GLP-1R in mouse pancreas.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0010.jpg)
![Immunohistochemistry: GLP-1R Antibody [NLS1205] - Human pancreas (Islets of Langerhans).](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Immunohistochemistry-NLS1205-img0008.jpg)
![Simple Western: GLP-1R Antibody [NLS1205] - Image shows a specific band for GLP-1R in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Simple-Western-NLS1205-img0005.jpg)
![Microarray: GLP-1R Antibody [NLS1205] - Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. Microarray image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLP-1R-Antibody-Microarray-NLS1205-img0013.jpg)
![Simple Western: GLP-1R Antibody - BSA Free [NLS1205] - Effect of the administration of exendin‐4 on eNOS expression. (a) Capillary western blot of total eNOS & phosphorylated eNOS expression in the rat retina. The red box indicates the target protein. (b & c) Fold change in the expression of total eNOS (b) & phosphorylated eNOS (c) in the rat retina (n = 7 in control group; n = 6 in Hp + PBS group; n = 9 in other groups). (d & e) Capillary western blot & fold change in the expression of GLP‐1 receptors (GLP‐1R) (d), PI3K, & Akt (e) in the rat retina. *P < .05, significantly different from CSF group; #P < .05significantly different from noradrenaline without exendin‐4 group. (f) NO content in human retinal microvascular endothelial cells (n = 6, 5, 5, 6, 7, 5, & 6 for group of control, OGD, OGD + Ex‐4, OGD + Ex‐4 + Ex‐9–39‐L, OGD + Ex‐4 + Ex‐9–39‐H, OGD + Ex‐4 + l‐NAME‐L, & OGD + Ex‐4 + l‐NAME‐H, respectively). One‐way ANOVA with LSD or Dunnett's T3 test were performed. C, control group; HP, high pressure injury group; EX‐4, exendin‐4; s.c., subcutaneous injection of exendin‐4; i.v., intravitreal injection of exendin‐4; od, eye drops of exendin‐4; OGD, oxygen glucose deprivation model; EX‐9–39‐L, low concentration of exendin‐9‐39 (10 nM); EX‐9–39‐H, high concentration of exendin‐9‐39 (20 nM); l‐NAME‐l, low concentration of l‐NAME (50 μM); l‐NAME‐H, high concentration of l‐NAME (100 μM) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32232832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nls1205_rabbit-polyclonal-glp-1r-antibody-3102024161368.jpg)
![Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0009.jpg "Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: WRN Antibody [NB100-471] - Analysis of WRN in untreated, asynchronous HeLa cells using anti-WRN antibody. The primary antibody was used at a dilution of 1:100, incubated for 1 hour at room temperature in 1XPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/WRN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-471-img0004.jpg)
![Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0005.jpg)
![Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.](http://images.novusbio.com/fullsize/WRN-Antibody-Western-Blot-NB100-471-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/WRN-Antibody-Immunoprecipitation-NB100-471-img0008.jpg)
![Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0076.jpg "Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (5, 15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiP53 antibody used for WB at0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: p53 Antibody [NB200-171] - FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/p53-Antibody-Immunohistochemistry-Paraffin-NB200-171-img0075.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- anti-KLH control IgG; Red- anti-HDMX.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0002.jpg)
![Chromatin Immunoprecipitation Sequencing: p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53. ChIP-seq validation performed by Active Motif, Carlsbad, CA.](http://images.novusbio.com/fullsize/p53-Antibody-Chromatin-Immunoprecipitation-Sequencing-NB200-171-img0073.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NB200-171-img0003.jpg)
![Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.](http://images.novusbio.com/fullsize/p53-Antibody-Flow-Cytometry-NB200-171-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p53 Antibody [NB200-171] - Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody NB200-171 used for IP at 3 ug per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody NB100-2204. For blotting immunoprecipitated P53, NB200-171 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NB200-171-img0077.jpg)
![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0004.jpg "Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunocytochemistry-NBP2-37364-img0011.jpg)
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - IHC staining of paraffin-embedded squamous cell carcinoma sample using Twist-1 antibody at 1:100 dilution. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-Paraffin-NBP2-37364-img0018.jpg)
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0014.jpg)
![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. Expected MW is 31.9 kDa.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0002.jpg)
![Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Western-Blot-NBP2-37364-img0003.jpg)
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-NBP2-37364-img0007.jpg)
![Immunohistochemistry-Paraffin: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Immunohistochemistry-NBP2-37364-img0009.jpg)
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0006.jpg)
![Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Cytometry-NBP2-37364-img0012.jpg)
![Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-Flow-Intracellular-NBP2-37364-img0013.jpg)
![ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Twist-1-Antibody-10E4E6-ELISA-NBP2-37364-img0001.jpg)
![Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37364_mouse-monoclonal-twist-1-antibody-10e4e6-31020241534384.jpg)
![Western Blot: Twist-1 Antibody (10E4E6) - BSA Free [NBP2-37364] - Validation of novel candidates at cellular DNA replication forks.BL cells (A) & LCL (B) cells were labeled with EdU for 15 min (active) followed by exposure to HU for 2 hours (stalled) prior to performing iPOND. Samples isolated by iPOND & 0.1% input samples were subjected to immunoblotting with indicated antibodies. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31841561), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37364_mouse-monoclonal-twist-1-antibody-10e4e6-31020241535631.jpg)