| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-316SS | |||
| Azide and BSA Free | NBP2-80684 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81734-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-61022-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-687SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-134SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56694SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-2525SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33122-100ug | |||
![Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Western-Blot-NB200-316-img0007.jpg "Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.")
![Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NB200-316-img0006.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Immunohistochemistry-Paraffin-NB200-316-img0010.jpg)
![Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Flow-Cytometry-NB200-316-img0008.jpg)
![Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Simple-Western-NB200-316-img0009.jpg)
![Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - Detection of Human and Mouse Matrin 3 on HeLa whole cell lysate using NB100-1761. Matrin 3 was also immunoprecipitated using another rabbit anti-Matrin 3 antibody and NB100-1559.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunoprecipitation-NB100-1761-img0013.jpg)
![Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0016.jpg)
![Immunohistochemistry-Paraffin: Matrin 3 Antibody [NB100-1761] - Section of human laryengeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-Paraffin-NB100-1761-img0015.jpg)
![Western Blot: Matrin 3 Antibody [NB100-1761] - The identification of host proteins immunoprecipitated with L1 ORF1p-FLAG. Validation of putative ORF1p-FLAG interacting proteins: Western blot images of the pJM101/L1.3FLAG and pJM101/L1.3 immunoprecipitation (IP) reactions. The pCEP4 lanes denote whole cell lysates derived from HeLa cells transfected with an empty pCEP4 vector (~ 1.0% input). Primary antibodies used to probe western blots are indicated to the left of the images. Immunoprecipitation reactions were conducted in either the absence (left) or presence (right) of RNaseA (10 ug/mL). The putative cellular functions of the ORF1p-FLAG interacting proteins are indicated on the right hand side of the blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1005121) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Western-Blot-NB100-1761-img0017.jpg)
![Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0020.jpg)
![Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of transverse sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0018.jpg)
![Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of f longitudinal sections from phenotypic Tg show an increase in Matrin 3 immunoreactivity compared to NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0019.jpg)
![Immunohistochemistry: Matrin 3 Antibody [NB100-1761] - Striking muscle pathology of gastrocnemius in Tg mice from Fo. 1579. Matrin 3 immunohistochemistry of NT transverse and longitudinal sections. * indicates rounded fiber. Arrow head indicates subsarcolemmal nucleus. Arrow indicates nuclear chain. Scale bars measure 25 um Image collected and cropped by CiteAb from the following publication (//actaneurocomms.biomedcentral.com/articles/10.1186/s40478-016-0393-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunohistochemistry-NB100-1761-img0021.jpg)
![Immunoprecipitation: Matrin 3 Antibody [NB100-1761] - IP of human fibroblasts. Image from verified customer review.](http://images.novusbio.com/fullsize/Matrin-3-Antibody-Immunoprecipitation-NB100-1761-img0008.jpg)
![Western Blot: Matrin 3 Antibody [NB100-1761] - TRAP150 inhibits the RRM-dependent binding activity of PSF, & inhibition of binding requires more than the minimal RRM binding domain. (A) UV crosslinking of ESS1 RNA with full-length PSF (PSF-FL) either alone (-), in the presence of BSA as a control or in the presence of increasing amounts of full-length FLAG-tagged TRAP150. (B) Same as panel (A), but competing binding of exRRMs of PSF with indicated truncations of GST-TRAP150 or GST alone. (C) Same as panel (A) but competing for binding of the minRRMs & hnRNP L by GST-TRAP(PID). (D) Western blots of immunoprecipitation of PSF from unstimulated (TRAP150 bound) & stimulated (TRAP150 unbound) JSL1 cells showing relative binding of TRAP150 & other known PSF-interacting partners. The source of the doublet for PSPC1 & p54nrb/NONO in stimulated cells is unknown, but is the same in input & IP samples. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1761_rabbit-polyclonal-matrin-3-antibody-31020241554516.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LONP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0019.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LONP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Simple Western: LONP1 Antibody [NBP1-81734] - Simple Western lane view shows a specific band for LONP1 in 0.2 mg/ml of h. Kidney (left) , NIH-3T3 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LONP1-Antibody-Simple-Western-NBP1-81734-img0003.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human duodenum shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0015.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human fallopian tube shows granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LONP1 Antibody [NBP1-81734] - PINK1 accumulates upon knockdown of Lon. Western blot analysis of Lon protease (Lon), Mitochondrial transcription factor A (TFAM), Heat shock protein 60 (Hsp60) and Actin in whole head homogenate from control and Lon deficient animals. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1004279), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: LONP1 Antibody [NBP1-81734] - Staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81734-img0009.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human tonsil shows granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0018.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/LONP1-Antibody-Western-Blot-NBP1-81734-img0020.jpg)
![Immunohistochemistry-Paraffin: LONP1 Antibody [NBP1-81734] - Staining of human pancreas shows granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/LONP1-Antibody-Immunohistochemistry-Paraffin-NBP1-81734-img0017.jpg)
![Simple Western: LONP1 Antibody [NBP1-81734] - Electropherogram image(s) of corresponding Simple Western lane view. LONP1 antibody was used at 1:20 dilution on h. Kidney and NIH-3T3 lysate(s).](http://images.novusbio.com/fullsize/LONP1-Antibody-Simple-Western-NBP1-81734-img0005.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - MTO1 defective cells exhibit proteostasis stress and an altered bioenergetic state. Representative immunoblot showing the expression of LONP1 in extracts of WT and MTO1 HF. Porin was used as a loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29348686/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-81734_rabbit-polyclonal-lonp1-antibody-2092023181153.jpg)
![Western Blot: LONP1 Antibody [NBP1-81734] - Altered mitochondrial features in cybrid cells carrying MELAS, MERRF, m.5514 A > G (mt-tRNATrp), m.1643A > G (mt-tRNAVal), & m.14487 T > C (ND6) mutations. (A) Representative western blot of LONP1, AFG3L2 & CLPP peptidases in mutant & wild type (WT) cybrid cells. The membrane was also probed with porin as a loading control. Full-length western blots & lower-exposure blots of porin are included in supplementary information. (B) Densitometric analysis of LONP1, AFG3L2 & CLPP normalized to porin & represented as fold change relative to WT (top). Quantitative data are from at least three independent experiments. Results from this analysis are also shown as a heatmap (bottom). The color & the corresponding value in log2 scale are depicted on the left. (C) Representative Blue Native-PAGE of OXPHOS complexes in mutant & WT cybrid cells. Full-length blots & lower-exposure blots for those with high contrast are included in supplementary information. (D) Densitometric analysis of OXPHOS complexes normalized to complex-II (loading control) & represented as fold change relative to WT. (E) Cellular ATP determination in mutant & WT cybrid cells. (F & G) Determination of Ca2+ (F) & ROS (G) by flow cytometry in mutant & WT cybrid cells with Fluo-3 & MitoSOX Red, respectively. All data are the mean ± SEM of at least three different experiments. Differences from WT values were found to be statistically significant at *p < 0.05, **p < 0.01 & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28740091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-81734_rabbit-polyclonal-lonp1-antibody-31020241612215.jpg)
![Immunocytochemistry/Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61022-img0002.jpg "Immunocytochemistry/Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022] - Pterostilbene significantly inhibits MTA1-dependent cell proliferation and angiogenesis and induces MTA1-targeted apoptosis in Ptenf/f mice(A) Representative Ki-67 (top, each panel), CD31 (middle, each panel) and VEGF-C (bottom, each panel) staining of the prostate tissues from Ptenf/f mice treated with vehicle and PTER, at indicated ages. Arrows indicate vessels. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/7841) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunohistochemistry-Paraffin-NB110-61022-img0003.jpg)
![Immunohistochemistry: VEGF-C Antibody [NB110-61022] - VEGFC Antibody [NB110-61022] - 4% PFA fixed and paraffin embedded mouse Lung tissue section was subjected to IHC staining of mouse VEGF-C.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunohistochemistry-NB110-61022-img0001.jpg)
![Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without KLK3, with & without the monoclonal antibody against KLK3 (5C7). Detection of VEGF-C in Western blots probed with antiserum 6 & 3/4, resulting in the detection of pro-VEGF-C (29/31 kDa) & activated, mature VEGF-C (21/23 kDa). The band marked by the asterisk likely represents the N-terminal propeptide (~15 kDa) which is detected by the antiserum 6. Note that for the image shown for antiserum 6, two different exposures of the same blot were merged (n = 3). (C, D) VEGF-C processed by KLK3 is biologically active in Ba/F3 cell assays, which translate activation of a hybrid VEGFR/EpoR receptor into cell survival (n = 2). Error bars indicate SD.10.7554/eLife.44478.004Figure 1—source data 1.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31099754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61022_rabbit-polyclonal-vegf-c-antibody-31020241620375.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0011.jpg "Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin Antibody diluted at a dilution of 1:3000. The bands indicate an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.")
![Immunohistochemistry-Frozen: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Smooth Muscle alpha staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100.Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Frozen-NBP1-30894-img0032.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel with the dilution of alpha-Smooth Muscle Actin Antibody at 1:1000. The band indicates an observed molecular weight of ~45 kDa and the theoretical molecular weight is 42 kDa.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Western-Blot-NBP1-30894-img0017.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0023.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0024.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0025.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0026.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat stomach. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0027.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Rat cervix. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0028.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Mouse duodenum. Alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/alpha-Smooth-Muscle-Actin-Antibody-Immunohistochemistry-Paraffin-NBP1-30894-img0031.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020241959359.jpg)
![Immunohistochemistry: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma stroma. alpha smooth muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242011442.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242075975.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041219.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205699.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242063310.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human normal colon tissue.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420122078.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-9102024205673.jpg)
![Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - alpha-Smooth Muscle Actin antibody detects alpha-Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse smooth muscle.alpha-Smooth Muscle Actin stained by alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-910202420104449.jpg)
![Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha-Smooth Muscle Actin antibody (NBP1-30894) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-91020242041283.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: alpha-Smooth Muscle Actin Antibody [NBP1-30894] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30894_rabbit-polyclonal-alpha-smooth-muscle-actin-antibody-1610202419414539.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0033.jpg "Western Blot: beta-Catenin Antibody [NBP1-32239] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Paraformaldehyde-fixed A431, using beta- Catenin antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0017.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - WNT10A/beta-catenin signaling is required for region-specific differentiation. Epithelial deletion of beta-catenin (g-h](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0043.jpg)
![Chromatin Immunoprecipitation (ChIP): beta-Catenin Antibody [NBP1-32239] - Cross-linked ChIP was performed with HCT116 chromatin extract and 5 ug of either control rabbit IgG or anti-beta Catenin antibody. The precipitated DNA was detected by PCR with primer set targeting to c-Myc promoter.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-32239-img0024.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Western blot for beta-catenin in HEK 293 cells treated with LiCl (10mM) for 1 hr. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0008.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0013.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEbeta Catenin antibody [N1N2-2], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP1-32239-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [N1N2-2], N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32239-img0032.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse urinary bladder diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0019.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse skin dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0020.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse colon dilution: 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0021.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0036.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human esophagus. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0037.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded human cervix. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0038.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0039.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat colon. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0040.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded rat duodenum. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0041.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - Paraffin-embedded mouse intestine. beta Catenin antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP1-32239-img0042.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - P-cadherin is co-localized with other junctional proteins at the RPE cell border in mice.Immunofluorescence of mouse RPE flat-mounts. Double staining: P-cadherin (red; A, E, I) & either ZO-1 (green; B), beta -catenin (green; F), or F-actin (green; J), with nuclear stain by DAPI (blue; C, G, K). Merged images (D, H, L) show the co-localization of P-cadherin with ZO-1 (tight junction), beta -catenin (adherens junction), & F-actin (adherens junction) at the cell-cell border. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202415171344.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241617146.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416171420.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618265.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - WNT10A/ beta -catenin signalling is required for region-specific differentiation.(a–d) Filiform papillae are present in Wnt10a−/− & inducible beta -catenin mutant dorsal tongue (yellow arrows), but horny structures & expression of hard keratins (in situ hybridization, purple signals) are decreased (red arrows). (e–l) Epithelial deletion of Wnt10a (e–f″,i,j) or beta -catenin (g–h″,k,l) induced from P25, P110 or P15 as indicated causes decreased expression of nuclear beta -catenin, LEF1 & HOXC13 (white arrows, LEF1+ proliferating cells; yellow arrows, HOXC13+ differentiating cells). (m) qPCR shows significantly decreased Hoxc13 levels in Wnt10a & beta -catenin mutant tongue epithelium. (n–r) IF & qPCR reveal reduced levels of KRT9 protein (n–q) & mRNA (r) in Wnt10a−/− & inducible beta -catenin mutant footpad epidermis. (s–v″) Co-IF for KRT9 & KRT10 in plantar epidermis from patients homozygous for WNT10A c.756+1G>A (s–t″) or WNT10A c.391G>A (u–v″) compared with similarly aged sex-matched controls. For qPCR, RNA levels were quantified in six control & six mutant (P40) or four control & four mutant (P20-100) samples with three technical replicates for each, & normalized to beta -actin mRNA. Significance was calculated with two-tailed Student's t-test. Error bars indicate s.e.m. Scale bar, 25 μm (e–l) or 50 μm (a–d,n–q,s–v″). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15397), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-31020241618934.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - Oxidative stress-induced dissociation of adherens junctions results in nuclear translocation of beta -catenin & an increase of EMT-related factors in mouse RPE.(A) Immunofluorescence of mouse RPE flat-mounts. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (a-c), 1 (d-f), 3 (g-i) & 7 (j-l). Double staining: beta -catenin (a, d, g, j), P-cadherin (b, e, h, k), & merged images with DAPI (c, f, i, l). The localization of beta -catenin & P-cadherin at the cell-cell border was significantly disrupted, & instead prominently detected on/in the nucleus on Day 3. (B) Immunofluorescence of mouse retinal sections with a focus on the RPE nuclei. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & the localization of beta -catenin (green) & P-cadherin (red) was analyzed along with nuclear stain by DAPI (blue) on Days 0 (m-o) & 3 (two representative nuclei; p-r & s-u). Double staining: beta -catenin (m, p, s), P-cadherin (n, q, t), & merged images with DAPI (o, r, u). On Day 3, beta -catenin was detected in the nuclei of mouse RPE. (C) Western blot analyses of mouse RPE proteins. Mice were injected with NaIO3 (15 mg/kg body weight) on Day 0, & RPE protein lysates were prepared on Days 0, 1, 3, & 7. The protein levels were analyzed using Western blotting with antibodies against P-cadherin, beta -catenin, SNAI1 (Snail), vimentin, & control beta -actin. The protein levels of beta -catenin & SNAI1 increased similarly on Day 1 following oxidative stress. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29338041), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-310202416165641.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242011415.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat liver.beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075974.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420122025.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420134025.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420104461.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using beta-Catenin antibody [N1N2-2], N-term NBP1-32239) at 1:100. dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420115056.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 1 day-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420564.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202461.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.Red: alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242001070.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects Ctnnb1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-9102024202198.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP1-32239) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242010443.jpg)
![Immunohistochemistry: beta-Catenin Antibody [NBP1-32239] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075983.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP1-32239] - beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta-Catenin stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-91020242075987.jpg)
![Western Blot: beta-Catenin Antibody [NBP1-32239] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP1-32239) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-910202420131247.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody [NBP1-32239] - Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-1610202419405728.jpg)
![beta-Catenin antibody [N1N2-2] detects beta-Catenin protein at cell membrane in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: beta-Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500.Red: alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunohistochemistry-paraffin-1411202518321620.jpg)
![beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) detects CTNNB1 protein by flow cytometry analysis. Sample: HeLa cell. Black: Unlabelled sample was used as a control. Red: beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-flow-cytometry-1411202518333124.jpg)
![beta-Catenin antibody [N1N2-2], N-term detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin protein stained by beta-Catenin antibody [N1N2-2], N-term (NBP1-32239) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-32239_rabbit-polyclonal-beta-catenin-antibody-immunocytochemistry-immunofluorescence-1411202518324119.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg "Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0004.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Flow-Cytometry-NB100-687-img0009.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0006.jpg)
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] - TUDCA induces intermediate states of mesenchymal & epithelial cells in primary cultures of rat HSC during hepatic differentiation.Freshly isolated rat HSC (a) exhibited typical lipid droplets that contained retinoids & (b) expressed the mesenchymal filament proteins vimentin (red) & desmin (green) as investigated by immunofluorescence. (c) Under control conditions HSC remained negative for K19 expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 μM TUDCA for 14 days (red). (e–g) K19 was found to be co-expressed with desmin & vimentin (green), which indicated the origin of epithelial progenitor cells from HSC. (h–k) Also K18, Afp & Mrp2 (red), which served as markers for hepatic differentiation, were co-expressed with vimentin & desmin (green) after 21 days of TUDCA treatment. Cells with different states of maturation were still found after 21 days of TUDCA treatment. Some cells developed into hepatocyte-like cells with (j) dominant K18 filaments (white arrows) while others remained immature with persisting desmin (red arrow) or (l) K19 protein residues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-687_rabbit-polyclonal-cytokeratin-19-antibody-31020241683916.jpg)
![Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Western-Blot-NB400-134-img0005.jpg "Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.")
![Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunocytochemistry-Immunofluorescence-NB400-134-img0008.jpg)
![Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification]](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Immunohistochemistry-Paraffin-NB400-134-img0009.jpg)
![Flow Cytometry: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.](http://images.novusbio.com/fullsize/SR-BI-SR-BII-Antibody-Flow-Cytometry-NB400-134-img0006.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0012.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block for30 min RT Predicted/Observed size: 18 kDa, 18 kDa.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. C) No Ab staining of injured tissue from an IL-1 beta KO.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-42767-img0004.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45C RT](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Immunohistochemistry-NBP1-42767-img0010.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0008.jpg)
![Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-Western-Blot-NBP1-42767-img0011.jpg)
![Immunoprecipitation: TRF-2 Antibody [NB100-56694] - Analysis of TRF-2 in HL60 cells. Lane 1. IP (mouse anti-TRF2). Lane 2. IP with control mouse IgG. Lane 3 IP with goat anti-TRF2. Lane 4. IP with pre-immune goat Ig. Lanes 1-4. WB. TRF2 is detected as a ~ 66 kD protein.](http://images.novusbio.com/fullsize/TRF-2-Antibody-Immunoprecipitation-NB100-56694-img0006.jpg "Immunoprecipitation: TRF-2 Antibody [NB100-56694] - Analysis of TRF-2 in HL60 cells. Lane 1. IP (mouse anti-TRF2). Lane 2. IP with control mouse IgG. Lane 3 IP with goat anti-TRF2. Lane 4. IP with pre-immune goat Ig. Lanes 1-4. WB. TRF2 is detected as a ~ 66 kD protein.")
![Immunohistochemistry-Paraffin: TRF-2 Antibody [NB100-56694] - Analysis of Human Tonsil using TRF-2 antibody.](http://images.novusbio.com/fullsize/TRF-2-Antibody-Immunohistochemistry-Paraffin-NB100-56694-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TRF-2 Antibody [NB100-56694] - U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF-2 (shTRF2) and selected with puromycin +/- Dox for a week. A) Western blot analysis of TRF-2 expression one week post selection. Membranes were also probed with anti-tubulin antibodies to show the input material loaded. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-2-Antibody-Knockdown-Validated-NB100-56694-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF-2 (shTRF2) and selected with puromycin +/- Dox for a week. One week post selection, +Dox cells were infected with HHV-6A for 48h and processed for IFA using anti-TRF-2 (green) and anti-IE2 (red). Cells with IE2 in punctate form and cells with large patchy IE2, likely to represent VRC, are shown. Nuclei are outlined by dashed lines. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRF-2-Antibody-Knockdown-Validated-NB100-56694-img0008.jpg)
![Western Blot: TRF-2 Antibody [NB100-56694] - TRF2 knockdown affect HHV-6A/B chromosomal integration.A) U2OS cells were transduced with lentiviral vectors expressing a scrambles shRNA (shCtrl) or a shTRF2. After a week of selection, cells were monitored for TRF2 expression by western blot. B) After a week of selection, shCtrl & shTRF2 treated cells were infected with HHV-6A or HHV-6B. After 30 days, DNA was isolated & the relative frequency of integration, relative to shCtrl set at 100%, estimated by ddPCR. *p<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-310202415345328.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - TRF2 expression in HHV-6A-infected U2OS cells.U2OS cells were infected with HHV-6A & analyzed for TRF2 & IE2 expression at 24h, 48h & 72h post-infection by dual color immunofluorescence. A) Representative immunofluorescence of TRF2 & IE2 expression in bystander & IE2 expressing cells at 24, 48h & 72h post infection. B) Mean relative TRF2 expression ± SD in uninfected (blue), IE2- (green-uninfected bystander) or IE2+ (red-infected) cells at 24h, 48h & 72h post infection. Each symbol represents the relative TRF2 expression from a single nucleus. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-310202415304273.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - Binding of TRF2 to viral DNA during HHV-6A/B infection.A) Schematic representation of the HHV-6A/B genome. The DR6 probe used for hybridization is shown in red. Uninfected & HHV-6A-infected HSB-2 cells (B-D) or uninfected & HHV-6B-infectd Molt-3 cells (E-F) were analyzed for TRF2 binding to viral DNA using ChIP. The input was hybridized with Alu probe to assess quantity of starting material. Anti-IgG (negative control), anti-PolII (positive control) or TRF2 antibodies were used for immunoprecipitation. B) QPCR detection of GAPDH DNA following ChIP. Results are expressed as fold increase over control IgG. C & E) Eluted DNA was hybridized with 32P-labeled Alu, telomeric (TTAGGG)3 or HHV-6A (DR6) probes. After hybridization the membranes were washed & exposed to X-ray films. D & F) Densitometric analysis of relative binding of TRF2 to telomeric & viral DNA. Results of one experiment representative of three are presented & are expressed as signal after normalization to input. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-3102024155376.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - TRF2 is required for IE2 localization with telomeres.U2OS cells were transduced with a lentiviral vector coding for a Dox inducible control shRNA (shCtrl) or a shRNA against TRF2 (shTRF2) & selected with puromycin +/- Dox for a week. A) Western blot analysis of TRF2 expression one week post selection. Membranes were also probed with anti-tubulin antibodies to show the input material loaded. B) One week post selection, +Dox cells were infected with HHV-6A for 48h & processed for IFA using anti-TRF2 (green) & anti-IE2 (red). Cells with IE2 in punctate form & cells with large patchy IE2, likely to represent VRC, are shown. Nuclei are outlined by dashed lines. C) The percentage of HHV-6A infected cells (from B) was estimated after counting a minimum of 700 cells & scoring the IE2+ ones. Results are expressed as mean %IE2+ cells ± SD. D) Mean percentage ± SD of IE2 localizing with telomeres in the presence (shCtrl +Dox) & absence (shTRF2 +Dox) of TRF2. Each dot represents the % of IE2 foci localizing with telomeres in one nucleus. ****p<0.0001. E) IF-FISH confocal images of shCtrl (+Dox) & shTRF2 (+Dox) cells analyzed for TRF2 (green), IE2 (red) & telomeres (cyan). Nuclei are outlined by dashed circles. Examples of IE2 localizing with telomeres (top row) or not found with telomeres (bottom row) are highlighted by the dashed polygons. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-31020241612657.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - Binding of TRF2 to viral DNA during HHV-6A/B infection.A) Schematic representation of the HHV-6A/B genome. The DR6 probe used for hybridization is shown in red. Uninfected & HHV-6A-infected HSB-2 cells (B-D) or uninfected & HHV-6B-infectd Molt-3 cells (E-F) were analyzed for TRF2 binding to viral DNA using ChIP. The input was hybridized with Alu probe to assess quantity of starting material. Anti-IgG (negative control), anti-PolII (positive control) or TRF2 antibodies were used for immunoprecipitation. B) QPCR detection of GAPDH DNA following ChIP. Results are expressed as fold increase over control IgG. C & E) Eluted DNA was hybridized with 32P-labeled Alu, telomeric (TTAGGG)3 or HHV-6A (DR6) probes. After hybridization the membranes were washed & exposed to X-ray films. D & F) Densitometric analysis of relative binding of TRF2 to telomeric & viral DNA. Results of one experiment representative of three are presented & are expressed as signal after normalization to input. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-310202415523926.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - A) A stick diagram of the IE2 protein with various domains identified is presented. B) Colocalization of HHV-6A IE2 protein with telomeres in the absence of viral DNA. U2OS cells were transfected with an empty vector, with IE2 expression vector or with IE2 delta 1290–1500 expression vector. Forty-eight hours later cells were processed for dual color immunofluorescence. Telomeres were labeled in cyan & IE2 in red. Nuclei are outlined by dashed lines. Examples of IE2 colocalizing with telomeres are presented in a 3D view (white arrows). C) The graph represents the mean ± SD % of WT IE2 & delta 1290–1500 IE2 localizing with telomeres. D) Lack of colocalization between HHV-6A p41 & telomeres in uninfected cells. U2OS cells were transfected with an empty vector or with a p41 expression vector. Forty-eight hours later cells were processed for dual color immunofluorescence. TRF2 was labeled green, p41 in red & nuclei outlined by a dashed line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-310202415541930.jpg)
![Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NB100-56694] - Colocalization of shelterin complex proteins & HHV-6A IE2 protein at VRC & cellular telomeres.A) U2OS cells were mock-infected or infected with HHV-6A for 48h after which cells were processed for IF-FISH. Telomeres were labeled in magenta, TRF2 in green & IE2 in red. The panels in the middle row show images of cells with IE2 patches overlapping with large, diffuse TRF2 & telomeric staining (rectangles). The panels in the third row represent infected cells with punctate IE2 pattern colocalizing with TRF2 & telomeres (dashed squares). The colocalization of IE2, TRF2 & telomeres are shown in both 2D & 3D images. B) Uninfected & HHV-6A-infected U2OS cells were transfected with an empty vector, a myc-tagged-TRF1 expression vector. Forty-eight hours later cells were processed for IF-FISH. TRF1 was labeled in green & IE2 in red. Nuclei were stained with DAPI. Images on the far right show 2D colocalization of TRF1 with IE2. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32320442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56694_goat-polyclonal-trf-2-antibody-31020241612621.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0007.jpg "Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in 293 cell lysates.")
![Immunocytochemistry/Immunofluorescence: ERO1L Antibody [NB100-2525] - Detection of ERO1L in Hela cells.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2525-img0006.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Analysis of ERO1L in mouse white adipose tissue using anti-ERO1L antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0008.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Thermal injury induces hepatic endoplasmic reticulum (ER) stress response in vivo. Western blots of ER stress proteins and ER calcium handling/chaperone proteins and/or their phosphoprotein levels in liver samples isolated from two control and six burned animals 24 hrs after burn injury. All Western blots are from the same animals. *Non-specific band. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/j.1582-4934.2008.00644.x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0009.jpg)
![Western Blot: ERO1L Antibody [NB100-2525] - Detection of ERO1 alpha in an endogenous 293T cell lysate.](http://images.novusbio.com/fullsize/ERO1L-Antibody-Western-Blot-NB100-2525-img0005.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD8-Antibody-Immunohistochemistry-Frozen-NBP2-29475-img0002.jpg "Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, and stained for or Ccdc88b (red) and CD8 (green). Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030607/) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: CD8 Antibody [NBP2-29475] - Mouse Ccdc88b expression in colon during DSS-induced colitis. Wild type (WT) mice were either not treated (NT) or given 3% DSS for 5 days followed by 3 days of water. aCcdc88b mRNA expression in distal colons of NT (n = 3) or DSS-treated WT mice (n = 3 for each time point) at the indicated days. Data represent expression relative to hprt±SEM (n = 3). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test) & are representative of one experiment. b Representative immunoblots for Ccdc88b protein detected in extracts from distal colons from NT or DSS-treated WT & Ccdc88Bmut mutant mice at indicated time points, & densitometric quantification of Ccdc88b immunoblot normalized to beta -actin±SEM (n = 3) (representative of one of two independent experiments); **P < 0.01 (two-tailed Student’s t-test). c Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at indicated times, & stained with antibodies against Ccdc88b (red), CD45 (green), & E-Cadherin (purple) & nuclei staining DAPI (blue). Scale bars, 100 μm. d Representative FACS plots & quantification of lamina propria cells stained for CD45 & for Ccdc88b antibodies for NT (n = 3) & DSS-treated WT mice (n = 4 for each time point) at indicated time points, data are representative of one experiment. e Representative confocal microscopy images of tissue sections from colon of NT or DSS-treated mice at day 8, & stained for Ccdc88b (red), CD3 (green) & CD11b (green); nuclei are stained with DAPI (blue). Scale bars, 100 μm. f same as in e for Ccdc88b (red), CD4 (green) & CD8 (green). Scale bars, 100 μm Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01381-y), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-29475_rabbit-polyclonal-cd8-antibody-imgenex-abx-160a-20ug-310202416113534.jpg)
