| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| Azide and BSA Free | NBP2-80831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-617SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54731-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44464 | |||
| BSA Free | NBP2-36754-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71704SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60984SS | |||
![Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Knockout-Validated-NBP2-60735-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Simple Western: LC3B Antibody (1251B) [NBP2-60735] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Simple-Western-NBP2-60735-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunocytochemistry-Immunofluorescence-NBP2-60735-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: LC3B Antibody (1251B) [NBP2-60735] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Western-Blot-NBP2-60735-img0010.jpg)
![Immunohistochemistry-Paraffin: LC3B Antibody (1251B) [NBP2-60735] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB (3,3'Diaminobenzidine) reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This LC3 antibody generated a diffused staining in all cell types (except the endothelial cells of blood vessels) and the signal was strongest in a subset of neuronal cells. A few cells depicted LC3 puncta formation/dotted staining in apparently autophagic cells.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Immunohistochemistry-Paraffin-NBP2-60735-img0011.jpg)
![<b>Biological Strategies Validation. </b>Flow (Intracellular): LC3B Antibody (1251B) [NBP2-60735] - HeLa cells were either (A) untreated or (B) treated with 50uM chloroquine for 24 hours. An intracellular stain was performed with anti-LC3B (1251B) antibody [Catalog # NBP2-60735] (blue) and a matched isotype control [Catalog # MAB1050] (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Flow-Intracellular-NBP2-60735-img0009.jpg)
![Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-RW109-Western-Blot-NB100-617-img0003.jpg "Western Blot: KAT3B/p300 Antibody (RW109) [NB100-617] - p300 detected in a HeLa nuclear extract using NB100-617 (1:1,000). ECL: 20 minute exposure.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Immunohistochemistry-Paraffin: HAP40 Antibody [NBP2-54731] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/HAP40-Antibody-Immunohistochemistry-Paraffin-NBP2-54731-img0011.jpg "Immunohistochemistry-Paraffin: HAP40 Antibody [NBP2-54731] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.")
![Immunohistochemistry-Paraffin: HAP40 Antibody [NBP2-54731] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HAP40-Antibody-Immunohistochemistry-Paraffin-NBP2-54731-img0008.jpg)
![Immunohistochemistry-Paraffin: HAP40 Antibody [NBP2-54731] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/HAP40-Antibody-Immunohistochemistry-Paraffin-NBP2-54731-img0009.jpg)
![Immunohistochemistry-Paraffin: HAP40 Antibody [NBP2-54731] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/HAP40-Antibody-Immunohistochemistry-Paraffin-NBP2-54731-img0010.jpg)
![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-132202416102266.jpg "ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-432024941537.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0003.jpg "Western Blot: Endothelial Lipase Antibody [NB400-118] - Human endothelial lipase detected in transfected 293 lysates using NB 400-118. (MW 57 kD)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-118] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-118-img0002.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-118] - Analysis of Endothelial Lipase in ECV304 cells.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-118-img0004.jpg)
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NB110-40424-img0009.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in human testis and skeletal muscle tissues. Corresponding VPS26A RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-VPS26A monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % inten](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Western-Blot-NBP2-36754-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U251 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0011.jpg)
![Western Blot: VPS26A Antibody (CL2287) [NBP2-36754] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Western-Blot-NBP2-36754-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in HeLa cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human cell line A431 showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in MCF7 cell line showing distinct staining of endosomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: VPS26A Antibody (CL2287) [NBP2-36754] - Staining in U2OS cell line with Anti-VSP26A monoclonal antibody, showing distinct staining of endosomes in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunocytochemistry-Immunofluorescence-NBP2-36754-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0013.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0014.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0015.jpg)
![Immunohistochemistry-Paraffin: VPS26A Antibody (CL2287) [NBP2-36754] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/VPS26A-Antibody-CL2287-Immunohistochemistry-Paraffin-NBP2-36754-img0016.jpg)
![ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Samples were spiked with high concentrations of Mouse NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80299_mouse-nf-l-elisa-kit-colorimetric-13220241617345.jpg "ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Samples were spiked with high concentrations of Mouse NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80299_mouse-nf-l-elisa-kit-colorimetric-432024855044.jpg)
![ELISA: Mouse NF-L ELISA Kit (Colorimetric) [NBP2-80299] - Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-80299_mouse-nf-l-elisa-kit-colorimetric-elisa-2442024141944..png)
![Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Western-Blot-NBP3-16729-img0004.jpg "Western Blot: RPTOR Antibody (9N0A4) [NBP3-16729] - Western blot analysis of extracts of various cell lines, using RPTOR Rabbit mAb (NBP3-16729) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: RPTOR Antibody (9N0A4) [NBP3-16729] - Immunohistochemistry of paraffin-embedded mouse kidney using RPTOR Rabbit mAb (NBP3-16729) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RPTOR-Antibody-9N0A4-Immunohistochemistry-Paraffin-NBP3-16729-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TP53INP2 Antibody [NBP1-71704] - TP53INP2 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/TP53INP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-71704-img0005.jpg "Immunocytochemistry/Immunofluorescence: TP53INP2 Antibody [NBP1-71704] - TP53INP2 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: TP53INP2 Antibody [NBP1-71704] - Staining of TP53INP2 in mouse prostate.](http://images.novusbio.com/fullsize/TP53INP2-Antibody-Immunohistochemistry-NBP1-71704-img0006.jpg)
![Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-2-Antibody-Simple-Western-NB110-60984-img0002.jpg "Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")