TP53INP2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: TP53INP2 Antibody [NBP1-71704] - TP53INP2 antibody was tested at 1:200 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: TP53INP2 Antibody [NBP1-71704] - Staining of TP53INP2 in mouse prostate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.6 mg/ml

TP53INP2 Antibody Summary

Immunogen
A genomic peptide made to a C-terminal region of the human TP53INP2 protein (within residues 100-220). [Swiss-Prot Q8IXH6]
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53INP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
Application Notes
This TP53INP2 antibody is useful for ICC/IF and IHC-P. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71704 in the following applications:

Reactivity Notes

Human and mouse. Immunogen has 81% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for TP53INP2 Antibody

  • C20orf110
  • chromosome 20 open reading frame 110
  • diabetes and obesity regulated
  • dJ1181N3.1
  • DKFZp434B2411
  • DKFZp434O0827
  • DOR
  • FLJ21759
  • FLJ23500
  • p53-inducible protein U
  • PINHPIG-U
  • tumor protein p53 inducible nuclear protein 2
  • tumor protein p53-inducible nuclear protein 2

Background

Tumor protein p53 inducible nuclear protein 2 is necessary for tumor cell invasive migration. Different splicing events in formation of this protein are key determinants of whether the tumor cell invasive morphology exists, and is probably mediated through a Golgi complex interaction. TP53INP2 also binds the Atg8-like proteins and VMP1 during autophagy, and generates scaffolding for autophagosome membranes. LC3 and LC3R also interact with TP53INP2 through an interaction with the autophagosome transmembrane protein VMP1. Thus, the effect of TP53INP2 on tumor growth seems to be both a direct effect on invasion and an indirect effect of insufficient autophagosome scaffolding and inflammation events in response to tumor formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for TP53INP2 Antibody (NBP1-71704) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71704:
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Images Ratings Applications Species Date Details
Western Blot TP53INP2 NBP1-71704
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reviewed by:
jennifer guadagno
WB Mouse 10/06/2015
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Summary

ApplicationWestern Blot
Sample TestedMouse Neuro2A whole cell lysate
SpeciesMouse
LotA-1

Blocking

Blocking Details10% non-fat dry milk in TBS-T (0.05% Tween), 1 Hr at Room temperature

Primary Anitbody

Dilution Ratio1:1000 Overnight at 4 degrees

Secondary Antibody

Secondary DescriptionGoat Anti-rabbit HRP
Secondary Concentration(1:5000) in 3% milk in TBS-T

Details

Detection NotesDetected by clarity ECL using Biorad Chemidoc. Exposed for 2, 5 min. Neg control- untransfected; Pos cont- INP2 overexpression

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for TP53INP2 Antibody (NBP1-71704) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71704AF405
Alexa Fluor 488 Labeled NBP1-71704AF488
Alexa Fluor 647 Labeled NBP1-71704AF647
Alexa Fluor 700 Labeled NBP1-71704AF700
Biotin Labeled NBP1-71704B
DyLight 405 Labeled NBP1-71704V
DyLight 488 Labeled NBP1-71704G
DyLight 550 Labeled NBP1-71704R
DyLight 650 Labeled NBP1-71704C
FITC Labeled NBP1-71704F

Additional TP53INP2 Products

TP53INP2 NBP1-71704

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Recent Reviews

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1
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jennifer guadagno
10/06/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol TP53INP2
Entrez
Uniprot