| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-60984SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73209 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52636-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43820 | |||
| BSA Free | NBP2-46618-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54702-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87754-25ul | |||
![Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Beclin-2-Antibody-Simple-Western-NB110-60984-img0002.jpg "Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Western-Blot-NBP2-02396-img0001.jpg "Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunocytochemistry-Immunofluorescence-NBP2-02396-img0008.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0007.jpg)
![Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Flow-Cytometry-NBP2-02396-img0010.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0002.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0003.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0004.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0005.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0006.jpg)
![Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunoprecipitation-NBP2-02396-img0009.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Western-Blot-NBP2-45802-img0008.jpg "Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0007.jpg)
![Western Blot: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Western-Blot-NBP2-45802-img0009.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0002.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0003.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0004.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0005.jpg)
![Immunohistochemistry: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunohistochemistry-NBP2-45802-img0006.jpg)
![Immunofluorescence: MEK2 Antibody (OTI1A2) [NBP2-45802] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MEK2.](http://images.novusbio.com/fullsize/MEK2-Antibody-OTI1A2-Immunofluorescence-NBP2-45802-img0001.jpg)
![Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Western-Blot-NBP2-00754-img0008.jpg "Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: S6K Antibody (1G4) [NBP2-00754] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunocytochemistry-Immunofluorescence-NBP2-00754-img0007.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Human pancreas tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0006.jpg)
![Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-S6K antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0002.jpg)
![Western Blot: S6K Antibody (1G4) [NBP2-00754] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S6K.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Western-Blot-NBP2-00754-img0003.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0004.jpg)
![Immunohistochemistry-Paraffin: S6K Antibody (1G4) [NBP2-00754] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-S6K mouse monoclonal antibody.](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Immunohistochemistry-Paraffin-NBP2-00754-img0005.jpg)
![Flow Cytometry: S6K Antibody (1G4) [NBP2-00754] - Analysis of Jurkat cells, using anti-S6K antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/S6K-Antibody-1G4-Flow-Cytometry-NBP2-00754-img0001.jpg)
![Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Western-Blot-NBP3-15854-img0003.jpg "Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Knockout-Validated-NBP3-15854-img0001.jpg)
![Immunoprecipitation: ATG5 Antibody (8L7X6) [NBP3-15854] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (NBP3-15854). Western blot was performed from the immunoprecipitate using ATG5 (NBP3-15854) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Immunoprecipitation-NBP3-15854-img0004.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726014.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517251919.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251720294.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726015.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517273524.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517213515.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-522025171896.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517284996.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517152727.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517172826.jpg)
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0013.jpg "Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.")
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Western-Blot-NBP2-78823-img0012.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. Clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0008.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0009.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4C and next incubated with 250ug of A549 lysate for 2 hours at 4C.](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0010.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: MEF, A549 cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (SMC-602) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..](http://images.novusbio.com/fullsize/VPS35-Antibody-7E4-Immunoprecipitation-NBP2-78823-img0011.jpg)
![Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0005.jpg "Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/UVRAG-Antibody-Immunohistochemistry-Paraffin-NBP1-18885-img0004.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: UVRAG Antibody [NBP1-18885] - Whole cell lysate from HeLa and 293T cells. UVRAG was also immunoprecipitated by rabbit anti-UVRAG antibody NBP1-18884.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0003.jpg)
![Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. Mutations of K430 and K437 decrease the binding of Beclin 1 and Rubicon. Immunoprecipitation of indicated proteins with Beclin 1 in HEK293T cells transfected with indicated Beclin 1 constructs. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms8215), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UVRAG-Antibody-Western-Blot-NBP1-18885-img0006.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0009.jpg "Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human tonsil shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0007.jpg)
![Western Blot: NF-M Antibody (2688) [NBP2-46618] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Western-Blot-NBP2-46618-img0008.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0001.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse trigeminal ganglion shows strong immunoreactivity in ganglion cells.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0002.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0003.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of rat brainstem shows strong positivity in neurons of mesencephalic trigeminal nucleus.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0004.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of mouse thalamus shows strong positivity in neural fibers.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0005.jpg)
![Immunohistochemistry: NF-M Antibody (2688) [NBP2-46618] - Staining of human cerebral cortex shows strong positivity in neural fibers and cell bodies.](http://images.novusbio.com/fullsize/NF-M-Antibody-2688-Immunohistochemistry-NBP2-46618-img0006.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0010.jpg "Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0007.jpg)
![Western Blot: ATG5 Antibody [NBP2-54702] - Western blot analysis in control (vector only transfected HEK293T lysate) and ATG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-54702-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP2-54702] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54702-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human tonsil shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0009.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0006.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells and lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0008.jpg)
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Analysis in human cerebral cortex and liver tissues. Corresponding NF-M RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: NF-M Antibody [NBP1-87754] - Analysis NBP1-87754 (A) shows similar pattern to independent antibody NBP1-87753 (B).](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87754-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NBP1-87754] - Staining of human cell line HEK 293 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87754-img0010.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0016.jpg)
![Western Blot: NF-M Antibody [NBP1-87754] - Analysis in human cell line HEK 293 and human cell line A-431.](http://images.novusbio.com/fullsize/NF-M-Antibody-Western-Blot-NBP1-87754-img0011.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebellum shows strong cytoplasmic positivity in white matter.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0014.jpg)
![Immunohistochemistry-Paraffin: NF-M Antibody [NBP1-87754] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NF-M-Antibody-Immunohistochemistry-Paraffin-NBP1-87754-img0015.jpg)