Beclin 2 Antibody - BSA Free

Images

 
Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.13 mg/ml

Beclin 2 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region (within residues 200-300) of the human Beclin 2 protein. [UniProt# A8MW95]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BECN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western 1:100
  • Western Blot 1:200-1:500
Application Notes
This Beclin 2 antibody is useful for Western blot analysis, where a band is seen at approx. 50 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26506105).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
Control Peptide
Beclin 2 Protein (NB110-60984PEP)
Publications
Read Publications using
NB110-60984 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33608268). Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.13 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Beclin 2 Antibody - BSA Free

  • BECN1 L1
  • BECN1L1
  • BECN1P1 beclin 1, autophagy related, pseudogene 1
  • BECN1P1

Background

Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beclin 2 Antibody (NB110-60984)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 4 of 4.
Publications using NB110-60984 Applications Species
Kim YJ, Kong Q, Yamamoto S et al. An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system Science advances Feb 1 2021 [PMID: 33608268] (Mouse)

Details:
Co-immunoprecipitaiton performed to analyze Becn2-GASP1 interactions in mouse Neuro2A cells.
Mouse
Song X, Zhu S, Chen P et al. AMPk-Mediated BECN1 Phosphorylation Promotes Ferroptosis by Directly Blocking System Xc-Activity. Curr. Biol. Jul 21 2018 [PMID: 30057310] (WB, Human) WB Human
Liu JL, Chen FF, Chang SF et al. Expression of Beclin Family Proteins Is Associated with Tumor Progression in Oral Cancer. PLoS ONE. 2015 Oct 28 [PMID: 26506105] (WB, IHC-P, Human)

Details:
Beclin 2 antibody was used for IHC-P analysis of tissues microarrays from oral cancer patients. The IHC was performed using Leica Bond-Max autostainer and the assay implicated 20 minutes 100C heat-induced antigen retrieval with Bond Epitope Retrieval Solution 1, endogenous peroxidase blocking with H2O2 for 5 minutes, use of primary at 1: 400 dilution with 30 minutes incubation, and detection using post-primary IgG linker and polymeric horseradish peroxidase IgG followed by DAB staining (Figure 1E-I). The antibody was also used for WB on SAS cells that were transfected with control plasmid or plasmids expressing flag-tagged Beclin 1 or Beclin 2 (Figure 4A).
WB, IHC-P Human
He R, Peng J, Yuan P et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy. 2015 May 04 [PMID: 25955014] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Beclin 2 Antibody (NB110-60984)

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Blogs on Beclin 2.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation ...  Read full blog post.

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Bioinformatics

Gene Symbol BECN2