Beclin 2 Antibody - BSA Free


Simple Western: Beclin 2 Antibody [NB110-60984] - Simple Western lane view shows a specific band for Beclin 2 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC
BSA Free
1.13 mg/ml

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Beclin 2 Antibody - BSA Free Summary

A synthetic peptide made to an internal region (within residues 200-300) of the human Beclin 2 protein. [UniProt# A8MW95]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 26506105)
  • Simple Western 1:100
  • Western Blot 1:200-1:500
Application Notes
A band is seen at approx. 50 kDa in Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
293 Whole Cell Lysate
Control Peptide
Beclin 2 Protein (NB110-60984PEP)
Read Publications using
NB110-60984 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33608268).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
1.13 mg/ml
Immunogen affinity purified

Alternate Names for Beclin 2 Antibody - BSA Free

  • BECN1 L1
  • BECN1L1
  • BECN1P1 beclin 1, autophagy related, pseudogene 1
  • BECN1P1


Beclin 2 is a novel coiled-coil protein related to the autophagic Beclin 1 protein. It is thought to interact with Bcl-2, an anti-apoptotic protein, and is believed to function in autophagy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Beclin 2 Antibody (NB110-60984)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.

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Showing Publications 1 - 4 of 4.
Publications using NB110-60984 Applications Species
Kim YJ, Kong Q, Yamamoto S et al. An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system Science advances 2021-02-01 [PMID: 33608268] (Mouse)

Co-immunoprecipitaiton performed to analyze Becn2-GASP1 interactions in mouse Neuro2A cells.
Song X, Zhu S, Chen P et al. AMPk-Mediated BECN1 Phosphorylation Promotes Ferroptosis by Directly Blocking System Xc-Activity. Curr. Biol. 2018-07-21 [PMID: 30057310] (WB, Human) WB Human
Liu JL, Chen FF, Chang SF et al. Expression of Beclin Family Proteins Is Associated with Tumor Progression in Oral Cancer. PLoS ONE. 2015-10-28 [PMID: 26506105] (WB, IHC-P, Human)

Beclin 2 antibody was used for IHC-P analysis of tissues microarrays from oral cancer patients. The IHC was performed using Leica Bond-Max autostainer and the assay implicated 20 minutes 100C heat-induced antigen retrieval with Bond Epitope Retrieval Solution 1, endogenous peroxidase blocking with H2O2 for 5 minutes, use of primary at 1: 400 dilution with 30 minutes incubation, and detection using post-primary IgG linker and polymeric horseradish peroxidase IgG followed by DAB staining (Figure 1E-I). The antibody was also used for WB on SAS cells that were transfected with control plasmid or plasmids expressing flag-tagged Beclin 1 or Beclin 2 (Figure 4A).
WB, IHC-P Human
He R, Peng J, Yuan P et al. Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy. 2015-05-04 [PMID: 25955014] (WB, Human) WB Human

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies


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Blogs on Beclin 2.

Beclin 2, a mammal-specific homolog of Beclin 1 with unique functional similarities and differences
Beclin 2 (BECN2) is also called Beclin-1-like protein 1/ BECN1P1 and it was recently identified by He et al 2013 as a mammal-specific homolog of the evolutionarily conserved protein Beclin 1 which is well established for its role in the regulation ...  Read full blog post.

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Gene Symbol BECN2