ZNF281 Antibody (4F7) - Azide and BSA Free

Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 expression in transfected 293T cell line by ZNF281 monoclonal antibody (M01), clone 4F7.Lane 1: ZNF281 transfected lysate(96.9 KDa).Lane 2: Non-transfected lysate.

Western Blot: ZNF281 Antibody (4F7) [H00023528-M01] - Analysis of ZNF281 over-expressed 293 cell line, cotransfected with ZNF281 Validated Chimera RNAi ( Cat # H00023528-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF281 monoclonal antibody (M01), clone 4F7 (Cat # H00023528-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: ZNF281 Antibody (4F7) [H00023528-M01] - Detection limit for recombinant GST tagged ZNF281 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Mycoplasma
• Clone: 4F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZNF281 Antibody (4F7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZNF281 Antibody (4F7) - Azide and BSA Free (H00023528-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ZNF281 (NP_036614, 787 a.a. ~ 893 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQS
• Localization: Nuclear
• Specificity: ZNF281 - zinc finger protein 281
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF281
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNF281 Antibody (4F7) - Azide and BSA Free

• FLJ20557
• GZP1
• ZBP-99
• zinc finger protein 416
• ZNF281
• ZNP-99

Background

This zinc finger protein is involved in transcriptional regulation. It represses the transcription of a number of genes including gastrin and ornithine decarboxylase. It binds to the G-rich box in the enhancer region of these genes and may also complex with Oct4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZNF281
• Entrez:
  • 23528(Human)
• Uniprot:
  • NP_036614(Human)