ZNF274 Antibody (4C12)

Images

 
Western Blot: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M01), clone 4C12.Lane 1: ZNF274 transfected lysate(74.2 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of monoclonal antibody to ZNF274 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: ZNF274 Antibody (4C12) [H00010782-M01] - Detection limit for recombinant GST tagged ZNF274 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF
Clone
4C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ZNF274 Antibody (4C12) Summary

Immunogen
ZNF274 (NP_598009, 420 a.a. ~ 531 a.a) partial recombinant protein with GST tag.QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT
Specificity
ZNF274 - zinc finger protein 274
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ZNF274
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24760766)
Publications
Read Publication using
H00010782-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNF274 Antibody (4C12)

  • GIOT2
  • GIOT-2
  • gonadotropin inducible transcription repressor-2
  • Gonadotropin-inducible ovary transcription repressor 2
  • gonadotropin-inducible transcription repressor 2
  • KOX7DKFZp434F1811
  • zinc finger protein 44 (KOX 7)
  • zinc finger protein 44
  • Zinc finger protein 55DKFZp686L21136
  • Zinc finger protein 58ZNF504
  • Zinc finger protein KOX7
  • zinc finger protein ZnFP12
  • ZNF
  • ZNF55
  • ZNF58

Background

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZNF274 Antibody (H00010782-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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ChIP
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for ZNF274 Antibody (H00010782-M01) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ZNF274
Entrez
OMIM
Uniprot