ZNF274 Antibody (4C12) - Azide and BSA Free

Western Blot: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of ZNF274 expression in transfected 293T cell line by ZNF274 monoclonal antibody (M01), clone 4C12.Lane 1: ZNF274 transfected lysate(74.2 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: ZNF274 Antibody (4C12) [H00010782-M01] - Analysis of monoclonal antibody to ZNF274 on HeLa cell . Antibody concentration 10 ug/ml.

ELISA: ZNF274 Antibody (4C12) [H00010782-M01] - Detection limit for recombinant GST tagged ZNF274 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, ChIP
• Clone: 4C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ZNF274 Antibody (4C12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ZNF274 Antibody (4C12) - Azide and BSA Free (H00010782-M01) is a monoclonal antibody validated for use in WB, ELISA, ICC/IF and ChIP. Anti-ZNF274 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ZNF274 (NP_598009, 420 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVHKKIHT
• Specificity: ZNF274 - zinc finger protein 274
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZNF274
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24760766)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNF274 Antibody (4C12) - Azide and BSA Free

• GIOT2
• GIOT-2
• gonadotropin inducible transcription repressor-2
• Gonadotropin-inducible ovary transcription repressor 2
• gonadotropin-inducible transcription repressor 2
• KOX7DKFZp434F1811
• zinc finger protein 44 (KOX 7)
• zinc finger protein 44
• Zinc finger protein 55DKFZp686L21136
• Zinc finger protein 58ZNF504
• Zinc finger protein KOX7
• zinc finger protein ZnFP12
• ZNF
• ZNF55
• ZNF58

Background

This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF274 Antibody (H00010782-M01)(2)

Publications using H00010782-M01

• Langouet M, Glatt-Deeley HR, Chung MS et al. Zinc finger protein 274 regulates imprinted expression of transcripts in Prader-Willi syndrome neurons. Hum Mol Genet 2017-12-07 [PMID: 29228278]
• Cruvinel E, Budinetz T, Germain N et al. Reactivation of Maternal SNORD116 Cluster via SETDB1 knockdown in Prader-Willi Syndrome iPSCs. Hum. Mol. Genet. 2014-04-23 [PMID: 24760766] (Chemotaxis, Human)

Bioinformatics

• Gene Symbol: ZNF274
• Entrez:
  • 10782(Human)
• OMIM:
  • 605467(Human)
• Uniprot:
  • NP_598009(Human)