ZNF177 Overexpression Lysate (Native)

Images

 
Western Blot: ZNF177 Overexpression Lysate (Adult Normal) [NBL1-18071] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZNF177.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ZNF177 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204451 and based on accession number NM_003451. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens zinc finger protein 177 (ZNF177), mRNA.
Gene
ZNF177

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
36.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ZNF177

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ZNF177 Overexpression Lysate (Native)

  • PIGX
  • zinc finger protein 177

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13761
Species: Hu
Applications: IHC, IHC-P
NBP2-33280
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33409
Species: Hu
Applications: WB, ICC/IF
AF6347
Species: Hu
Applications: WB
AF4509
Species: Hu
Applications: WB, ICC
MAB4620
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ICC
NBP2-31787
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-24716
Species: Hu, Pm
Applications: WB, IHC-P
NBP1-97946
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-45672
Species: Hu, Mu, Rt
Applications: WB
NBP1-90290
Species: Hu
Applications: IHC, IHC-P
NBP2-24493
Species: Hu, Mu, Bv, Ch, Eq, Op, Pm
Applications: WB
NBP2-52414
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, ICC, IF
NBP1-89334
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37343
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NLS1927
Species: Hu, Po, Bv, Gt
Applications: IHC-P
AF1310
Species: Hu
Applications: WB, IHC, IP

Publications for ZNF177 Lysate (NBL1-18071) (0)

There are no publications for ZNF177 Lysate (NBL1-18071).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ZNF177 Lysate (NBL1-18071) (0)

There are no reviews for ZNF177 Lysate (NBL1-18071). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ZNF177 Lysate (NBL1-18071) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ZNF177 Products

Bioinformatics Tool for ZNF177 Lysate (NBL1-18071)

Discover related pathways, diseases and genes to ZNF177 Lysate (NBL1-18071). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ZNF177 Lysate (NBL1-18071)

Discover more about diseases related to ZNF177 Lysate (NBL1-18071).
 

Pathways for ZNF177 Lysate (NBL1-18071)

View related products by pathway.

PTMs for ZNF177 Lysate (NBL1-18071)

Learn more about PTMs related to ZNF177 Lysate (NBL1-18071).

Blogs on ZNF177

There are no specific blogs for ZNF177, but you can read our latest blog posts.

Customers Who Bought This Also Bought

BMP-7 [Unconjugated]
354-BP-010/CF
ZNF177 Antibody
NBP1-86523
LRPAP Antibody
NBP1-89334

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ZNF177 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol ZNF177