ZFP106 Antibody

Western Blot: ZFP106 Antibody [NB100-74653] - Detection of Human and Mouse ZFP106 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells, and whole cell lysate (50 ug) from Jurkat, Ramos, and Mouse CT26.WT cells. Antibody: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Immunoprecipitation: ZFP106 Antibody [NB100-74653] - Detection of human ZFP106 by western blot of immunoprecipitates. Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-ZFP106 antibody NB100-74653 used for IP at 6 ug per reaction. ZFP106 was also immunoprecipitated by rabbit anti-ZFP106 antibody NB100-74654. For blotting immunoprecipitated ZFP106, NB100-74653 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZFP106 Antibody Summary

• Description: Novus Biologicals Rabbit ZFP106 Antibody (NB100-74653) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1300 and 1350 of human zinc finger protein 106 using the numbering given in entry NP_071918.1 (GeneID 64397).
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZFP106
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZFP106 Antibody

• DKFZp451A239
• FLJ34610
• SH3BP3
• SH3-domain binding protein 3
• zfp-106
• zinc finger protein 106 homolog (mouse)
• zinc finger protein 106 homolog
• Zinc finger protein 474
• ZNF474FLJ45841

Background

Zinc finger protein homolog 106 (ZFP106) is a C2H2-type zinc finger protein that also contains 2 WD repeats. ZFP106 is highly expressed in striated muscle, brown fat, and developing brain and is regulated by myogenin and nuclear respiratory factor-1 (NRF-1). ZFP106 localizes to the nucleus in association with nucleolar transcriptional machinery. The WD40 repeat in ZFP106 appears to facilitate targeting of ZFP106 to the nucleolus and to mediate the interaction between ZFP106 and testis-specific gene 118 (TSF118). A two-hybrid screen has identified testis-specific Y-encoded-like protein (TSPYL) as a ZFP106 interacting protein. These data suggest an important role for ZFP106 in testis development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZFP106
• Entrez:
  • 64397(Human)
• Uniprot:
  • NP_071918.1(Human)