ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate

Lane 1: ZCCHC11 transfected lysate (34.1 KDa) Lane 2: Non-transfected lysate.

ZCCHC11 transfected lysate.

• Reactivity: Hu
• Applications: WB

ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate Summary

• Description: Plasmid: pCMV-ZCCHC11 full-length
• Specificity: ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: TUT4

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-ZCCHC11 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate

• DKFZp779C1943
• EC 2.7.7.52
• KIAA0191FLJ42878
• PAP associated domain containing 3
• PAPD3
• terminal uridylyltransferase 4
• TUT4
• TUTase 4
• Zinc finger CCHC domain-containing protein 11
• zinc finger, CCHC domain containing 11

Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TUT4