ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate

Images

 
Lane 1: ZCCHC11 transfected lysate (34.1 KDa) Lane 2: Non-transfected lysate.
ZCCHC11 transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate Summary

Description
Quality control test: Transient overexpression cell lysate was tested with Anti-ZCCHC11 antibody by Western Blots. Plasmid: pCMV-ZCCHC11 full-length
Specificity
ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate(Denatured)
Gene
TUT4

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZCCHC11/TUT4 293T Cell Transient Overexpression Lysate

  • DKFZp779C1943
  • EC 2.7.7.52
  • KIAA0191FLJ42878
  • PAP associated domain containing 3
  • PAPD3
  • terminal uridylyltransferase 4
  • TUT4
  • TUTase 4
  • Zinc finger CCHC domain-containing protein 11
  • zinc finger, CCHC domain containing 11

Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol TUT4