ZBTB5 Antibody - BSA Free

Western Blot: ZBTB5 Antibody [NBP1-76518] - Mouse brain tissue lysate with ZBTB5 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunohistochemistry of ZBTB5 in human brain tissue with ZBTB5 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ZBTB5 Antibody - BSA Free [NBP1-76518] - Immunofluorescence of ZBTB5 in human brain tissue with ZBTB5 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZBTB5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZBTB5 Antibody - BSA Free (NBP1-76518) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human ZBTB6. The immunogen is located within the last 50 amino acids of ZBTB5. Amino Acid Squence: KRFSQSSHLYKHSKTT
• Specificity: At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZBTB5
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  ZBTB5 Protein (NBP1-76518PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZBTB5 Antibody - BSA Free

• KIAA0354
• zinc finger and BTB domain containing 5
• zinc finger and BTB domain-containing protein 5

Background

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB5 was identified though sequence analysis as a POZ domain Kruppel-like zinc finger (POK) protein. Further experiments indicated that it binds DNA and can directly repress transcription of the cell cycle arrest gene p21. ZBTB5 can also interact with co-repressor histone deacetylase complexes such as BCoR, NCoR, and SMRT via its POZ domain, resulting in deacetylation of histones Ac-H3 and Ac-H4 at the proximal promoter. ZBTB5 stimulates both cell proliferation and cell cycle progression, suggesting that it may act as a potential proto-oncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB5
• Uniprot:
  • NP_055687(Human)