Yes Overexpression Lysate

Images

 
Western Blot: Yes1 Overexpression Lysate (Adult Normal) [NBL1-17928] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Yes1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Yes Overexpression Lysate Summary

Description

Yes Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208432

Accession#: NM_005433

Protein Tag: C-MYC/DDK
Gene
YES1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
60.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Yes Overexpression Lysate

  • cellular yes-1 protein
  • c-yes
  • EC 2.7.10
  • EC 2.7.10.2
  • HsT441
  • P61-YES
  • Proto-oncogene c-Yes
  • proto-oncogene tyrosine-protein kinase Yes
  • v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
  • Yamaguchi sarcoma oncogene
  • Yes
  • YES1

Background

This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol YES1