XRCC4 Antibody

Western Blot: XRCC4 Antibody [H00007518-B01P] - Analysis of XRCC4 expression in transfected 293T cell line by XRCC4 polyclonal antibody. Lane 1: XRCC4 transfected lysate(36.96 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [H00007518-B01P] - Analysis of purified antibody to XRCC4 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: XRCC4 Antibody [H00007518-B01P] - Analysis of purified antibody to XRCC4 on formalin-fixed paraffin-embedded human adrenal gland. (antibody concentration 3 ug/ml)

Product Discontinued

XRCC4 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: XRCC4 (NP_071801.1, 1 a.a. - 336 a.a.) full-length human protein. MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
• Specificity: XRCC4 - X-ray repair complementing defective repair in Chinese hamster cells 4,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: XRCC4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for XRCC4 Antibody

• complementing defective, repair in Chinese hamster
• X-ray repair complementing defective repair in Chinese hamster cells 4
• X-ray repair cross-complementing protein 4

Background

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XRCC4
• Entrez:
  • 7518(Human)
• Uniprot:
  • NP_071801.1(Human)