XAF1 Antibody

Product Discontinued

XAF1 Antibody Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1. The immunogen is located within amino acids 230 - 280 of XAF-1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1-5 ug/ml
• Application Notes: XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 1-2 mg/ml. Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN).
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XAF1 Antibody

• BIRC4-binding protein
• BIRC4BPBIRC4 binding protein
• HSXIAPAF1
• XIAP associated factor 1
• XIAPAF1XIAP-associated factor 1

Background

XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XAF1
• Uniprot:
  • CAA68030(Human)