WSB2 Overexpression Lysate

Images

 
Western Blot: WSB2 Overexpression Lysate (Adult Normal) [NBL1-17884] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WSB2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WSB2 Overexpression Lysate Summary

Description

WSB2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202375

Accession#: NM_018639

Protein Tag: C-MYC/DDK
Gene
WSB2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WSB2 Overexpression Lysate

  • CS box-containing WD protein
  • MGC10210
  • SBA2
  • WD repeat and SOCS box containing 2
  • WD repeat and SOCS box containing protein 2
  • WD repeat and SOCS box-containing 2
  • WD repeat and SOCS box-containing protein 2
  • WSB-2

Background

This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

596-MR
Species: Mu
Applications: BA
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
MAB3811
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
NB100-616
Species: Hu, Mu, Md, Pm, Rt
Applications: ChIP, EM, Flow-IC, Flow, ICC/IF, IP, In vitro, WB
NBP2-19860
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB400-144
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
594-ML/CF
Species: Mu
Applications: BA
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, Simple Western, WB
NBP1-06398
Species: Hu, Pm, Rt
Applications: IB, ICC/IF, IHC, IHC-P, WB
DTM400
Species: Hu
Applications: ELISA
NBP1-84853
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-82049
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF284
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
NBP1-92060
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF-252-PB
Species: Hu
Applications: IHC, WB
AF3194
Species: Hu
Applications: ICC, Simple Western, WB
NBP2-55678
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-83698
Species: Hu
Applications: ICC/IF, IHC, IHC-P

Publications for WSB2 Lysate (NBL1-17884) (0)

There are no publications for WSB2 Lysate (NBL1-17884).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for WSB2 Lysate (NBL1-17884) (0)

There are no reviews for WSB2 Lysate (NBL1-17884). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WSB2 Lysate (NBL1-17884) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional WSB2 Products

Bioinformatics Tool for WSB2 Lysate (NBL1-17884)

Discover related pathways, diseases and genes to WSB2 Lysate (NBL1-17884). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for WSB2 Lysate (NBL1-17884)

Discover more about diseases related to WSB2 Lysate (NBL1-17884).
 

Pathways for WSB2 Lysate (NBL1-17884)

View related products by pathway.

Research Areas for WSB2 Lysate (NBL1-17884)

Find related products by research area.

Blogs on WSB2

There are no specific blogs for WSB2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our WSB2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol WSB2