MAP7 Antibody

Western Blot: MAP7 Antibody [NBP1-84853] - Analysis in control (vector only transfected HEK293T lysate) and MAP7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: MAP7 Antibody [NBP1-84853] - Staining of human cell line A-431 shows localization to cytosol & microtubules. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human lymph node.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human colon shows high expression.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining in human cerebral cortex and skeletal muscle tissues.. Corresponding MAP7 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-MAP7 antibody NBP1-84853 (A) shows similar protein distribution across tissues to independent antibody NBP1-84852 (B).

Immunohistochemistry-Paraffin: MAP7 Antibody [NBP1-84853] - Staining of human testis.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MAP7 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IAKGALTGGTEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MAP7 Protein (NBP1-84853PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MAP7 Antibody

• E-MAP-115dJ325F22.2 (microtubule-associated protein 7 (EMAP115, E-MAP-115))
• ensconsin
• Epithelial microtubule-associated protein of 115 kDa
• MAP-7
• microtubule-associated protein 7EMAP115

Background

The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP7 Antibody (NBP1-84853)(1)

Publication using NBP1-84853

• Fukuzawa R, Anaka MR, Morison IM, Reeve AE. The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour. PLoS ONE. 2017-10-17 [PMID: 29040332] (Human)

Bioinformatics

• Gene Symbol: MAP7