| Reactivity | Hu, Mu, Bt, Ca, Pm, PmSpecies Glossary |
| Applications | IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic 18 amino acid peptide from internal region of human Wnt-8a. |
| Epitope | Internal |
| Specificity | Human Wnt-8a. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Predicted Species | Mouse (94%), Monkey (94%), Primate (100%), Bat (100%), Canine (94%). Backed by our 100% Guarantee. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WNT8A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry: beta-Catenin Antibody (12F7) [NBP1-54467] - An intracellular stain was performed on SK-MEL-28 cells with beta-Catenin Antibody [12F7] NBP1-54467B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Flow-Cytometry-NBP1-54467-img0012.jpg)
![Western Blot: beta-Catenin Antibody (12F7) [NBP1-54467] - Analysis of beta Catenin expression in 1) HepG2, 2) MCF7, and 3) Cos7 whole cell lysates using NBP1-54467.](http://images.novusbio.com/images/beta-Catenin-Antibody-(12F7)-Western-Blot-NBP1-54467-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (12F7) [NBP1-54467] - IF beta-Catenin staining of mouse brain tissue. This image submitted by a verified customer review.](http://images.novusbio.com/images2/beta-Catenin-Antibody-(12F7)-Immunohistochemistry-NBP1-54467-img0011.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0001.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0002.jpg)
![Immunohistochemistry: Wnt3 Antibody (1G7) [NBP2-46367] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/Wnt3-Antibody-(1G7)-Immunohistochemistry-NBP2-46367-img0003.jpg)
![Flow Cytometry: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/images2/Wnt-2b-Antibody-(JB48-34)-Flow-Cytometry-NBP2-75713-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Wnt-2b-Antibody-(JB48-34)-Immunocytochemistry-Immunofluorescence-NBP2-75713-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Wnt-2b Antibody (JB48-34) [NBP2-75713] - Staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/Wnt-2b-Antibody-(JB48-34)-Immunocytochemistry-Immunofluorescence-NBP2-75713-img0003.jpg)
![Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining of human tonsil shows low expression as expected.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89679-img0015.jpg)
![Immunohistochemistry-Paraffin: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Staining in human duodenum and tonsil tissues using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89679-img0016.jpg)
![Western Blot: HNF-4 alpha/NR2A1 Antibody [NBP1-89679] - Analysis in human cell lines Caco-2 and PC-3 using anti-HNF4A antibody. Corresponding HNF4A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/images2/HNF-4-alpha-NR2A1-Antibody-Western-Blot-NBP1-89679-img0017.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for Wnt-8a Antibody (NBP3-14523)Discover more about diseases related to Wnt-8a Antibody (NBP3-14523).
| Pathways for Wnt-8a Antibody (NBP3-14523)View related products by pathway.
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PTMs for Wnt-8a Antibody (NBP3-14523)Learn more about PTMs related to Wnt-8a Antibody (NBP3-14523).
| Research Areas for Wnt-8a Antibody (NBP3-14523)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | WNT8A |
![Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunocytochemistry-Immunofluorescence-NBP1-51575-img0006.jpg)
![Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Immunohistochemistry-Paraffin-NBP1-51575-img0007.jpg)
![Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.](http://images.novusbio.com/images2/Wnt-1-Antibody-(10C8)-Western-Blot-NBP1-51575-img0008.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Analysis of anti-WNT10A antibody with human pancreatic islet at concentration 10 ug/ml.](http://images.novusbio.com/images2/Wnt-10a-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59026-img0002.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Immunohistochemical analysis of human prostate.](http://images.novusbio.com/images2/Wnt-10a-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59026-img0003.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Immunohistochemical analysis of human stomach.](http://images.novusbio.com/images2/Wnt-10a-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59026-img0004.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/images2/LEF1-Antibody-(3H5)-Western-Blot-H00051176-M01-img0006.jpg)
![Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/images2/LEF1-Antibody-(3H5)-Sandwich-ELISA-H00051176-M01-img0007.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.](http://images.novusbio.com/images2/LEF1-Antibody-(3H5)-Western-Blot-H00051176-M01-img0008.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
