Wnt-7b Antibody - BSA Free

Western Blot: Wnt-7b Antibody [NBP2-93501] - Analysis of extracts of various cell lines, using Wnt-7b at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: Wnt-7b Antibody [NBP2-93501] - Analysis of U2OS cells using Wnt-7b at dilution of 1:100. Blue: DAPI for nuclear staining.

Product Discontinued

Wnt-7b Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human WNT7B (NP_478679.1). TAACSQGNLSNCGCDREKQGYYNQAEGWKWGGCSADVRYGIDFSRRFVDAREIKKNARRLMNLHNNEAGRKVLEDRMQLECKCHGVSGSCTTKTCWTTLPKFREVGHLLKEKYNAAVQVEVVRASRLRQPTFLRIKQLRSYQKPMETDLVYIEKSPNYCEEDAATGSVGTQGRLCNRTSPGADGCDTMCCGRGYNTHQYTKVWQCNCKFHWCCFVKCNTCSERTEVFTCK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT7B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Wnt-7b Antibody - BSA Free

• protein Wnt-7b
• wingless-type MMTV integration site family, member 7B
• Wnt7b
• Wnt-7b

Background

Wnt7b is a gene that codes for a protein that is 349 amino acids in length and approximately 39 kDa in weight that is a ligand for members of the frizzled family of transmembrane receptors that may function to facilitate the development of discrete region of tissues. Current studies are being done on several diseases and disorders linked to this gene including carcinoma, leukemia, fainting, hermaphroditism, melanoma, prostatitis, neuronitis, and colorectal cancer. Wnt7b has also been shown to have interactions with FZD1, FZD10, FZD5, GPC3, and LRP5 in pathways such as the GSK3 signaling, colorectal cancer metastasis, DNA damage response, and GPCR ligand binding pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WNT7B