CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Wnt-6 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human Wnt-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4190) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4109-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68908-25ul | |||
![SDS-Page: Recombinant Human Wnt-6 Protein [H00007475-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Wnt-6-Protein-SDS-Page-H00007475-P01-img0002.jpg "SDS-Page: Recombinant Human Wnt-6 Protein [H00007475-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024939127.jpg "Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024940838.jpg)
![Western Blot: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024941416.jpg)
![Immunohistochemistry-Paraffin: Wnt-6 Antibody [NB110-55627] - Analysis of anti-WNT6 antibody with human breast, epithelium cells.](http://images.novusbio.com/fullsize/Wnt-6-Antibody-Immunohistochemistry-Paraffin-NB110-55627-img0001.jpg "Immunohistochemistry-Paraffin: Wnt-6 Antibody [NB110-55627] - Analysis of anti-WNT6 antibody with human breast, epithelium cells.")
![Immunohistochemistry-Paraffin: Wnt-6 Antibody [NBP2-68908] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Wnt-6-Antibody-Immunohistochemistry-NBP2-68908-img0001.jpg "Immunohistochemistry-Paraffin: Wnt-6 Antibody [NBP2-68908] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.")
![Western Blot: Wnt-6 Antibody [NBP1-62305] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Wnt-6-Antibody-Western-Blot-NBP1-62305-img0003.jpg "Western Blot: Wnt-6 Antibody [NBP1-62305] - 293T cells lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Wnt6 Overexpression Lysate (Adult Normal) [NBL1-17875] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Wnt6.](http://images.novusbio.com/fullsize/Wnt6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17875-img0002.jpg "Western Blot: Wnt6 Overexpression Lysate (Adult Normal) [NBL1-17875] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Wnt6.")
![Immunohistochemistry-Paraffin: Wnt-6 Antibody [NBP3-14521] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast, epithelium cells, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-6 Antibody-Immunohistochemistry-Paraffin-NBP3-14521-img0001.jpg "Immunohistochemistry-Paraffin: Wnt-6 Antibody [NBP3-14521] - Immunohistochemistry of formalin-fixed, paraffin-embedded human breast, epithelium cells, after heat-induced antigen retrieval.")
![Western Blot: Wnt-6 Antibody [H00007475-D01P] - Analysis of WNT6 expression in transfected 293T cell line by WNT6 polyclonal antibody.Lane 1: WNT6 transfected lysate(39.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Wnt-6-Antibody-Western-Blot-H00007475-D01P-img0003.jpg "Western Blot: Wnt-6 Antibody [H00007475-D01P] - Analysis of WNT6 expression in transfected 293T cell line by WNT6 polyclonal antibody.Lane 1: WNT6 transfected lysate(39.70 KDa).Lane 2: Non-transfected lysate.")
