Recombinant Human Wnt-2 Protein

Images

 
SDS-Page: Recombinant Human Wnt-2 Protein [H00007472-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PA

Order Details

Recombinant Human Wnt-2 Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-360 of Human WNT2 full-length ORF

Source: Wheat Germ (in vitro)

Amino Acid Sequence:MNAPLGGIWLWLPLLLTWLTPEVNSSWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT

Details of Functionality
This protein is not active and should not be used for experiments requiring activity.
Protein/Peptide Type
Recombinant Protein
Gene
WNT2

Applications/Dilutions

Application Notes
This protein has not been tested for any functionality. Product may contain endotoxins and is not suitable for use with live cells.
Publications
Read Publications using H00007472-P01.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Wnt-2 Protein

  • INT1L1
  • INT1L1secreted growth factor
  • Int-1-like protein 1
  • Int-1-related protein
  • IRP
  • IRPprotein Wnt-2
  • wingless-type MMTV integration site family member 2
  • Wnt2
  • Wnt-2

Background

WNT2( AAH29854, 1 a.a. - 361 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Wnt-2 Recombinant Protein (H00007472-P01)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Wnt-2 Recombinant Protein (H00007472-P01). (Showing 1 - 1 of 1 FAQs).

  1. I'm thinking to buy your product WNT2 recombinant protein (H00007472-P01). According to datasheet, this product is not active. However, in the reference papers, the authors used it in their experiments that require activity. I'm wondering how Novus generated this product (from bacteria? cultured cell?) and have you checked activity by yourself (and if so, how?).
    • We (Novus) have not actually tested the activity of WNT2 Recombinant Protein (H00007472-P01), and neither has the manufacturer of this particular product, a company called Abnova. This is a recombinant protein produced using Abnova's in vitro wheat germ expression system. Because the protein is not native, and we have not proven its activity, we include the statement on the datasheet, as we cannot guarantee it will be suitable for functional studies (and so it is recommended for applications such as a standard in western blot). However, this does not mean that H00007472-P01 is definitely not active, just that we do not currently have sufficient evidence to guarantee that it is active. Because 'Func' is not stated on the datasheet, if you do choose to use the protein in this application, if you shared your findings with us (whether the antibody proved suitable or not) by writing a review for the product, as a thank you we would give you a voucher code for the cost of the product - this is our Innovator's Reward program.

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Blogs on Wnt-2

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Bioinformatics

Gene Symbol WNT2
Entrez