Wnt-10a Antibody - BSA Free

Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Immunohistochemical analysis of human melanoma in lymph node.

Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Analysis of anti-WNT10A antibody with human pancreatic islet at concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Immunohistochemical analysis of human prostate.

Immunohistochemistry-Paraffin: Wnt-10a Antibody - BSA Free [NB100-59026] - Immunohistochemical analysis of human stomach.

• Reactivity: Hu, Bt, Bv, Ca, Pm, Pm, Rb, Rt, Ha, Ma-Op
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Wnt-10a Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from internal region of human Wnt-10a.
• Epitope: Internal region of human protein
• Specificity: Human WNT10A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PTPRD (44%).
• Predicted Species: Rat (93%), Hamster (93%), Opossum (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WNT10A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: Use in Immunohistochemistry reported in scientific literature (PMID: 20195877)..
• Control:

  Wnt-10a Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Wnt-10a Antibody - BSA Free

• FLJ14301
• protein Wnt-10a
• SSPS
• wingless-type MMTV integration site family, member 10A
• Wnt10a
• Wnt-10a

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene over expression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-10a Antibody (NB100-59026)(1)

Publication using NB100-59026

• Hakim SG, Kosmehl H, Sieg P, Trenkle T, Jacobsen HC, Attila Benedek G, Ribbat J, Driemel O. Altered expression of cell-cell adhesion molecules ?-catenin/E-cadherin and related Wnt-signaling pathway in sporadic and syndromal keratocystic odontogenic tumors. Clin Oral Investig;15(3):321-8. 2011-06-01 [PMID: 20195877] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: WNT10A
• Entrez:
  • 80326(Human)
• Uniprot:
  • Q9GZT5(Human)