WISP2 Antibody (3D10) - Azide and BSA Free

Western Blot: WISP2 Antibody (3D10) [H00008839-M09] - Analysis of WISP2 expression in transfected 293T cell line by WISP2 monoclonal antibody (M09), clone 3D10. Lane 1: WISP2 transfected lysatE (26.8 KDa). Lane 2: Non-transfected lysate.

Immunoprecipitation: WISP2 Antibody (3D10) [H00008839-M09] - Analysis of WISP2 transfected lysate using anti-WISP2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with WISP2 rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 3D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

WISP2 Antibody (3D10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: WISP2 (AAH17782.1, 24 a.a. ~ 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLCPTPCTCPWPPPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLHVCDASQGLVCQPGAGPGGRGALCLLAEDDCSCEVNGRLYREGETFQPHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF
• Specificity: WISP2 - WNT1 inducible signaling pathway protein 2
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCN5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for WISP2 Antibody (3D10) - Azide and BSA Free

• CCN5
• Connective tissue growth factor-like protein
• Connective tissue growth factor-related protein 58
• CT58CTGF-Lwnt-1 signaling pathway protein 2
• CTGFL
• CTGF-L
• WISP2
• WISP-2
• WNT1 inducible signaling pathway protein 2
• WNT1-inducible-signaling pathway protein 2

Background

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCN5
• Entrez:
  • 8839(Human)
• OMIM:
  • 603399(Human)
• Uniprot:
  • AAH17782(Human)