WIPI1 Overexpression Lysate (Native)

Images

 
Western Blot: WIPI1 Overexpression Lysate (Adult Normal) [NBL1-17853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WIPI1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WIPI1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208094 and based on accession number NM_017983. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens WD repeat domain, phosphoinositide interacting 1 (WIPI1), mRNA.
Gene
WIPI1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WIPI1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WIPI1 Overexpression Lysate (Native)

  • Atg18 protein homolog
  • Atg18
  • ATG18A
  • FLJ10055
  • WD repeat domain phosphoinositide-interacting protein 1
  • WD repeat domain, phosphoinositide interacting 1
  • WD40 repeat protein interacting with phosphoinositides of 49 kDa
  • WD40 repeat protein Interacting with phosphoInositides of 49kDa
  • WIPI 49 kDa
  • WIPI-1 alpha
  • WIPI-1
  • WIPI49ATG18

Background

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-53818
Species: Hu, Mu, Rt, Po, Bv, Pm, Xp, Ze
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, PLA
NBP1-88879
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
NBP1-88883
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-55202
Species: Hu
Applications: WB, IHC, IHC-P
AF853
Species: Mu
Applications: WB, IHC
H00200576-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-24594
Species: Hu, Mu, Rt
Applications: WB, IHC-P
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89865
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2331
Species: Hu, Mu, Rt, Ca, Fi, Pl, Ze
Applications: WB, Simple Western, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, SB
NB100-56398
Species: Hu
Applications: WB, ICC/IF
NBP2-24738
Species: Hu, Mu, Pm
Applications: WB, IHC-P
NBP1-80838
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
H00007001-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP
26160002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-15104
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for WIPI1 Lysate (NBL1-17853) (0)

There are no publications for WIPI1 Lysate (NBL1-17853).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for WIPI1 Lysate (NBL1-17853) (0)

There are no reviews for WIPI1 Lysate (NBL1-17853). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for WIPI1 Lysate (NBL1-17853) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional WIPI1 Products

Bioinformatics Tool for WIPI1 Lysate (NBL1-17853)

Discover related pathways, diseases and genes to WIPI1 Lysate (NBL1-17853). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for WIPI1 Lysate (NBL1-17853)

Discover more about diseases related to WIPI1 Lysate (NBL1-17853).
 

Pathways for WIPI1 Lysate (NBL1-17853)

View related products by pathway.

PTMs for WIPI1 Lysate (NBL1-17853)

Learn more about PTMs related to WIPI1 Lysate (NBL1-17853).

Blogs on WIPI1.

WIPI1 - An essential regulator of early autophagosome assembly
WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...  Read full blog post.

Customers Who Bought This Also Bought

WIPI1 Partial Protein
H00055062-Q01-10ug
LC3A Antibody
NB100-2331
WIPI1 Antibody
NBP1-88878
ATG5 Antibody
NB110-53818

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our WIPI1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol WIPI1