WIPF1/WIP Antibody

Western Blot: WIPF1/WIP Antibody [NB100-68147] - Analysis of WIPF1/WIP antibody using this antibody at 0.03 ug/ml in Human Tonsil. Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: WIPF1/WIP Antibody [NB100-68147] - HEK293 overexpressing WIPF1 and probed with (mock transfection in first lane).

Product Discontinued

WIPF1/WIP Antibody Summary

• Immunogen: Peptide with sequence C-KLARNESRSGSNRRE corresponding to C-Terminus according to NP_001070737.1.
• Epitope: KLARNESRSGSNRRE
• Specificity: Both variants represent identical proteins
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WIPF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human lymph node, spleen and tonsil lysates (calculated MW of 51.3 kDa band according to NP_003378.3 and NP_001070737.1). In transfected HEK293 transiently expressing WIPF1 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  WIPF1/WIP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WIPF1/WIP Antibody

• WAS/WASL interacting protein family, member 1
• WAS/WASL-interacting protein family member 1
• WASP-interacting protein
• WASPIP
• WIPMGC111041
• WiskProtein PRPL-2
• WiskPRPL-2

Background

WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WIPF1/WIP Antibody (NB100-68147)(1)

Publication using NB100-68147

• Massarwa R, Carmon S, Shilo BZ, Schejter ED. WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila. Dev Cell 2007-04-01 [PMID: 17419994]

Bioinformatics

• Gene Symbol: WIPF1
• Entrez:
  • 7456(Human)
  • 215280(Mouse)
  • 117538(Rat)
• Uniprot:
  • NP_003378.3(Human)