WHSC1 Antibody (CL1063) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Analysis in human testis and liver tissues using NBP3-44042 antibody. Corresponding WHSC1 RNA-seq data are presented for the same tissues.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human rectum shows strong nuclear positivity in glandular cells.

Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Staining of U-251 cells using the Anti-WHSC1 monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL1063
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

WHSC1 Antibody (CL1063) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of O96028, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NSD2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for WHSC1 Antibody (CL1063) - Azide and BSA Free

• EC 2.1.1.43
• FLJ23286
• IL5 promoter REII region-binding protein
• KIAA1090
• MGC176638
• MMSETNSD2Multiple myeloma SET domain-containing protein
• multiple myeloma SET domain containing protein type III
• nuclear receptor binding SET domain protein 2
• Nuclear SET domain-containing protein 2
• probable histone-lysine N-methyltransferase NSD2
• Protein trithorax-5
• REIIBP
• trithorax/ash1-related protein 5
• TRX5
• WHS
• Wolf-Hirschhorn syndrome candidate 1 protein
• Wolf-Hirschhorn syndrome candidate 1

Background

WHSC1 encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSD2