WFDC5 Overexpression Lysate (Native)

Images

 
Western Blot: WFDC5 Overexpression Lysate (Adult Normal) [NBL1-17842] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WFDC5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

WFDC5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204523 and based on accession number NM_145652. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens WAP four-disulfide core domain 5 (WFDC5), mRNA.
Gene
WFDC5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
10.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for WFDC5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for WFDC5 Overexpression Lysate (Native)

  • dJ211D12.5
  • PRG5protease inhibitor WAP1
  • Putative protease inhibitor WAP1
  • WAP four-disulfide core domain 5
  • WAP four-disulfide core domain protein 5
  • WAP1p53-responsive gene 5 protein

Background

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol WFDC5