Recombinant Human WDR68 His Protein

SDS-Page: Recombinant Human WDR68 Protein [NBP1-72421] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human WDR68 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-295 of Human WDR68
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVYAMNWSVR PDKRFRLALG SFVEEYNNKV QLVGLDEESS EFICRNTFDH PYPTTKLMWI PDTKGVYPDL LATSGDYLRV WRVGETETRL ECLLNNNKNS DFCAPLTSFD WNEVDPYLLG TSSIDTTCTI WGLETGQVLG RVNLVSGHVK TQLIAHDKEV YDIAFSRAGG GRDMFASVGA DGSVRMFDLR HLEHSTIIYE DPQHHPLLRL CWNKQDPNYL ATMAMDGMEV VILDVRVPCT PVARLNNHRA CVNGIAWAPH SSCHICTAAD DHQALIWD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DCAF7
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.4M UREA, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human WDR68 His Protein

• DDB1 and CUL4 associated factor 7
• DDB1- and CUL4-associated factor 7
• HAN11AN11
• human anthocyanin
• seven-WD-repeat protein of the AN11 family-1
• SWAN-1
• WD repeat domain 68
• WD repeat-containing protein 68
• WD repeat-containing protein An11 homolog
• WDR68
• WD-repeat protein

Background

DCAF7, also known as DDB1-and CUL4-associated factor, involved in craniofacial development. This protein acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. This protein associates with DIAPH1 and controls GLI1 transcriptional activity. This protein could be involved in normal and disease skin development. Recombinant human DCAF7 protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during conventional chromatography purification process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DCAF7