VPS37C Antibody

Images

 
Western Blot: VPS37C Antibody [NBP1-31068] - Sample(30 ug of whole cell lysate)A:Raji12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: VPS37C Antibody [NBP1-31068] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS37C protein stained by VPS37C antibody [N1], N-term diluted at 1:500. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

VPS37C Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VPS37C. The exact sequence is proprietary.
Localization
Late endosome membrane, Peripheral membrane protein (Probable)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS37C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (87%), Rat (87%), Bovine (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for VPS37C Antibody

  • ESCRT-I complex subunit VPS37C
  • FLJ20847
  • hVps37C
  • PML39
  • vacuolar protein sorting 37 homolog C (S. cerevisiae)
  • vacuolar protein sorting 37C (yeast)
  • vacuolar protein sorting 37C
  • vacuolar protein sorting-associated protein 37C

Background

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VPS37C