VPS37C Antibody

Western Blot: VPS37C Antibody [NBP1-31068] - Sample(30 ug of whole cell lysate)A:Raji12% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: VPS37C Antibody [NBP1-31068] - Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS37C protein stained by VPS37C antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Product Discontinued

VPS37C Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human VPS37C. The exact sequence is proprietary.
• Localization: Late endosome membrane, Peripheral membrane protein (Probable)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS37C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for VPS37C Antibody

• ESCRT-I complex subunit VPS37C
• FLJ20847
• hVps37C
• PML39
• vacuolar protein sorting 37 homolog C (S. cerevisiae)
• vacuolar protein sorting 37C (yeast)
• vacuolar protein sorting 37C
• vacuolar protein sorting-associated protein 37C

Background

VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: VPS37C