VPS29 Antibody

Product Discontinued

VPS29 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AGHRLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPW
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPS29
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Signal Stimulation
  • Simple Western 1:5
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 28954865). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/I FFixation Permeabilization: Use PFA/Triton X-100. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35045281). Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for VPS29 Antibody

• DC15
• DC7
• DKFZp564F0223
• EC 3.1.3.3
• FLJ20492
• hVPS29
• PEP11 homolog
• PEP11
• retromer protein
• vacuolar protein sorting 29 (yeast homolog)
• vacuolar protein sorting 29 (yeast)
• vacuolar protein sorting 29 homolog (S. cerevisiae)
• vacuolar protein sorting-associated protein 29
• vacuolar sorting protein VPS29/PEP11
• Vesicle protein sorting 29
• x 007 protein

Background

VPS29 is an essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS29 Antibody (NBP1-85288)(3)

Publications using NBP1-85288

• Qureshi YH, Berman DE, Marsh SE et al. The neuronal retromer can regulate both neuronal and microglial phenotypes of Alzheimer's disease Cell reports 2022-01-18 [PMID: 35045281] (WB, Mouse)
• Nogueras-Ortiz C, Roman-Vendrell C, Mateo-Semidey GE et al. Retromer stops beta-arrestin 1 mediated signaling from internalized cannabinoid 2 receptors. Mol. Biol. Cell. 2017-09-27 [PMID: 28954865] (WB)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: VPS29