VPS26A Overexpression Lysate

Images

 
Western Blot: VPS26 Overexpression Lysate (Adult Normal) [NBL1-17740] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS26.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

VPS26A Overexpression Lysate Summary

Description

VPS26A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC205353

Accession#: NM_004896

Protein Tag: C-MYC/DDK
Gene
VPS26A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for VPS26A Overexpression Lysate

  • FLJ12930
  • HB58
  • Hbeta58
  • hVPS26
  • PEP8A
  • vacuolar protein sorting 26 (yeast homolog)
  • vacuolar protein sorting 26 homolog A (S. pombe)
  • vacuolar protein sorting 26 homolog A (yeast)
  • vacuolar protein sorting-associated protein 26A
  • Vesicle protein sorting 26A
  • VPS26vacuolar protein sorting 26 (yeast)

Background

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for VPS26A Lysate (NBL1-17740) (0)

There are no publications for VPS26A Lysate (NBL1-17740).
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol VPS26A