VPS16 Antibody

Product Discontinued

VPS16 Antibody Summary

• Immunogen: Synthetic peptide: C-KQHNKYEAKK, representing the internal region of the human protein according to NP_072097.2; NP_536339.1; NP_536338.1
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_072097.2 and NP_536338.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VPS16
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-6 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA detection limit 1:64000
  • Western Blot
• Application Notes: Preliminary experiments gave bands at approx 55kDa and 26kDa in human placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 94.7kDa for isoform 1 according to NP_072097.2, of 36.8kDa for isoform 2 according to NP_536339.1 and of 78.3kDa for isoform 3 according to NP_536338.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Expected to recognise all 3 reported isoforms (NP_072097.2, NP_536339.1 and NP_536338.1).Western Blot was reported in scientific literature.

Reactivity Notes

Human, Mouse, Rat, Dog, Cow

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for VPS16 Antibody

• hVPS16
• vacuolar protein sorting 16 homolog (S. cerevisiae)
• vacuolar protein sorting protein 16
• vacuolar protein sorting-associated protein 16 homolog

Background

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinctorganelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved invesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps16 protein. The mammalian classC Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, maymediate vesicle trafficking steps in the endosome/lysosome pathway. Two transcript variants encoding differentisoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VPS16 Antibody (NB100-2440)(1)

Publication using NB100-2440

• Thi EP, Lambertz U, Reiner NE. Class IA Phosphatidylinositol 3-Kinase p110alpha Regulates Phagosome Maturation PLoS One 2012-01-01 [PMID: 22928013] (WB, Human)

Bioinformatics

• Gene Symbol: VPS16
• Entrez:
  • 64601(Human)
• Uniprot:
  • NP_072097.2(Human)