VMAT2 Antibody (NBP1-69750)

Images

 
Western Blot: VMAT2 Antibody [NBP1-69750] - WB analysis of VMAT2 in rat striatal tissue lysate. Image courtesy of anonymous customer product review.
Immunohistochemistry: VMAT2 Antibody [NBP1-69750] - IHC staining of VMAT2 in mouse brain cortex.
Western Blot: VMAT2 Antibody [NBP1-69750] - Analysis of VMAT2 in human brain lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.36 mg/ml

VMAT2 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human VMAT2 protein (within residues 30-200). [Swiss-Prot Q05940]
Localization
Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC18A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This VMAT2 antibody is useful for IHC and Western blot where a doublet is seen ~60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-69750 in the following applications:

Publications
Read Publications using
NBP1-69750 in the following applications:

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.36 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for VMAT2 Antibody

  • MGC120477
  • MNAT
  • monoamine neurotransmitter transporter
  • Monoamine transporter
  • SLC18A2
  • solute carrier family 18 (vesicular monoamine), member 2
  • Solute carrier family 18 member 2
  • SVAT
  • SVMT
  • SVMTMGC120478
  • synaptic vesicular amine transporter
  • VAT2
  • VAT2MGC26538
  • vesicle monoamine transporter type 2
  • vesicle monoamine/H+ antiporter
  • Vesicular amine transporter 2
  • VMAT2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VMAT2 Antibody (NBP1-69750)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: IHC-Fr, WB.


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IHC-Fr
(1)
WB
(1)
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Rat
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP1-69750 Applications Species
Reichard EE, Nanaware-Kharade N, Gonzalez GA et al. PEGylation of a High-Affinity Anti-(+)Methamphetamine Single Chain Antibody Fragment Extends Functional Half-Life by Reducing Clearance Pharm. Res [PMID: 27620175] (WB, Rat)

Details:
Used the HRP form of this antibody.
WB Rat
Vuong HE. Heterogeneous functional organization of somatostatin- and dopamine-containing wide-field amacrine cells in mouse retina Thesis, Graduate Student Researcher, UCLA: Physiological Science 0671 Retrieved from: <a href='http://escholarship.org/uc/item/74b76099'target='_blank'>escholarship.org</a> Aug 10 2015 7:59AM (IHC-Fr, Mouse) IHC-Fr Mouse

Review for VMAT2 Antibody (NBP1-69750) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-69750:
Filter by Applications
WB
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot VMAT2 NBP1-69750
Enlarge
reviewed by:
Anony- mous
WB Rat 09/15/2011
View

Summary

ApplicationWestern Blot
Sample TestedE13 rat RN46A-B14 cells from medullary raphe, Sample Amount: 10ug rat striatal vesicular tissue, 25 ug cell vesicular fraction
SpeciesRat
LotA
CommentsWe give the VMAT2 antibody 4 stars. It worked very well for us in rat brain tissue.

Blocking

Blocking DetailsBlocking Buffer: 0.2% TBS-T with 5% milk

Primary Anitbody

Dilution RatioDilution Ratio: 1:5,000, Incubation Dilution Buffer: 0.2% TBS-T w/ 5% milk, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat anti-rabbit, Secondary Ab Dilution Ratio: 1:2500
Secondary Manufacturer Cat#Chemicon

Details

Detection NotesHyGlow HRP Detection, Pos Ctrl: Saline treated rat striatum, vesicular fraction preperation, Specific Bands: 60 kDa

Comments

CommentsWe give the VMAT2 antibody 4 stars. It worked very well for us in rat brain tissue.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VMAT2 Antibody (NBP1-69750) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-69750AF405
Alexa Fluor 488 Labeled NBP1-69750AF488
Alexa Fluor 647 Labeled NBP1-69750AF647
Alexa Fluor 700 Labeled NBP1-69750AF700
Biotin Labeled NBP1-69750B
DyLight 405 Labeled NBP1-69750V
DyLight 488 Labeled NBP1-69750G
DyLight 550 Labeled NBP1-69750R
DyLight 650 Labeled NBP1-69750C
FITC Labeled NBP1-69750F
HRP Labeled NBP1-69750H

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Contact Information

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anony- mous
09/15/2011
Application: WB
Species: Rat

Bioinformatics

Gene Symbol SLC18A2
Entrez
Uniprot

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