VMAT2 Antibody

Western Blot: VMAT2 Antibody [NB100-1479] - (2ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry: VMAT2 Antibody [NB100-1479] - Negative Control showing staining of paraffin embedded Human Adrenal Gland, with no primary antibody.

Immunohistochemistry: VMAT2 Antibody [NB100-1479] - (6ug/ml) staining of paraffin embedded Human Adrenal Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu, Rt, Mu, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VMAT2 Antibody Summary

• Immunogen: Peptide with sequence C-SYPIGEDEESESD corresponding to C-Terminus according to NP_003045.2.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SLC18A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 6 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/mL
• Application Notes: ICC/IF reactivity reported in scientific literature (PMID: 30567483).
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30567483).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VMAT2 Antibody

• MGC120477
• MNAT
• monoamine neurotransmitter transporter
• Monoamine transporter
• SLC18A2
• solute carrier family 18 (vesicular monoamine), member 2
• Solute carrier family 18 member 2
• SVAT
• SVMT
• SVMTMGC120478
• synaptic vesicular amine transporter
• VAT2
• VAT2MGC26538
• vesicle monoamine transporter type 2
• vesicle monoamine/H+ antiporter
• Vesicular amine transporter 2
• VMAT2

Background

VMAT2 (Vesicular amine transporter 2) acts to accumulate cytosolic monoamines into synaptic vesicles. Its function is essential to the correct activity of the monoaminergic systems that have been implicated in several human neuropsychiatric disorders such as dementia with Lewy bodies. The transporter is a site of action of important drugs, including reserpine and tetrabenazine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VMAT2 Antibody (NB100-1479)(2)

Publications using NB100-1479

• Ahmad MF, Ferland D, Ayala-Lopez N et al. Perivascular Adipocytes Store Norepinephrine by Vesicular Transport Arterioscler. Thromb. Vasc. Biol. 2018-12-20 [PMID: 30567483] (ICC/IF, Rat)
• Erickson JD, Eiden LE, Hoffman BJ. Expression cloning of a reserpine-sensitive vesicular monoamine transporter. Proc Natl Acad Sci U S A 1992-11-15 [PMID: 1438304]

Bioinformatics

• Gene Symbol: SLC18A2
• Entrez:
  • 6571(Human)
  • 214084(Mouse)
  • 25549(Rat)
• Uniprot:
  • Q05940()