![Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (mam82) - Chimeric [NBP3-11975] - Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of mam82 (NBP3-11975) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11975, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-mam82-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11975-img0001.jpg "Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (mam82) - Chimeric [NBP3-11975] - Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of mam82 (NBP3-11975) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11975, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.")
| Reactivity | MuSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC, B/N |
| Clone | mam82 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit VISTA/B7-H5/PD-1H Antibody (mam82) - Chimeric - Azide and BSA Free (NBP3-11975) is a recombinant monoclonal antibody validated for use in IHC, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | The antibody was generated by immunising a VISTA/B7-H5/PD-1H-KO mouse with mouse VISTA/B7-H5/PD-1H Ig fusion protein and adjuvant. |
| Specificity | This antibody specifically binds VISTA/B7-H5/PD-1H. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | VSIR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for VISTA/B7-H5/PD-1H Antibody (NBP3-11975)Find related products by research area.
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| Gene Symbol | VSIR |
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