![ELISA: VISTA/B7-H5/PD-1H Antibody (13F3) - Chimeric [NBP3-12054] - ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using NBP3-12054. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-13F3-Chimeric-ELISA-NBP3-12054-img0001.jpg "ELISA: VISTA/B7-H5/PD-1H Antibody (13F3) - Chimeric [NBP3-12054] - ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using NBP3-12054. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.")
| Reactivity | MuSpecies Glossary |
| Applications | ELISA, Flow, B/N |
| Clone | 13F3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA/B7-H5/PD-1H-RFP, and VISTA/B7-H5/PD-1H-Ig fusion protein. |
| Specificity | This antibody is specific for murine VISTA/B7-H5/PD-1H. |
| Isotype | IgG Lambda |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | VSIR |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for VISTA/B7-H5/PD-1H Antibody (NBP3-12054)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | VSIR |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
