visinin-like 1 Antibody

Western Blot: visinin-like 1 Antibody [NBP2-25169] - Analysis of different tissue lysates using rabbit pAb to visinin-like Protein 1 (VLP1), NBP2-25169, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at ~20kDa corresponds to the VLP1 protein.

Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody [NBP2-25169] - Neuron-glia cell culture stained with NBP2-25169 (red), monoclonal antibody to GFAP: NBP1-05197 (green) and DNA (blue). NBP2-25169 reveals strong staining throughout some small neurons in soma and all processes, accumulation is seen in some hotspots along neurites. GFAP appears only in astrocytes.

Immunohistochemistry Free-Floating: visinin-like 1 Antibody [NBP2-25169] - Analysis of rat cerebellum section stained with rabbit pAb to VLP1, NBP2-25169, dilution 1:2,000 in green, and costained with mouse mAb to calretinin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The VLP1 antibody reveals protein expressed in granule cells membranes and their synapses in both the granular and molecular layer of the cerebellum. The calretinin antibody stains the cytoplasm of neurons in the nuclear and molecular layers of cerebellum.

Western Blot: visinin-like 1 Antibody [NBP2-25169] - Bovine cerebellum homogenate. Note the strong clean band running at 22 kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-FrFl
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

visinin-like 1 Antibody Summary

• Immunogen: Full length recombinant human VLP1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VSNL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:2000
  • Immunohistochemistry Free-Floating 1:2000
  • Immunohistochemistry 1:2000
  • Western Blot 1:10000
• Application Notes: This visinin-like 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~22 kDa.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for visinin-like 1 Antibody

• HLP3Hippocalcin-like protein 3
• HPCAL3
• HUVISL1
• VILIP
• VILIP-1
• VILIPVLP-1
• visinin-like 1
• visinin-like protein 1
• VISL1
• VSNL1

Background

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VSNL1
• Entrez:
  • 7447(Human)
• OMIM:
  • 600817(Human)
• Uniprot:
  • P62760(Human)