visinin-like 1 Antibody (3A9)

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167] - Analysis of different tissue lysates using mouse mAb to visinin-like protein 1 (VLP1), NBP2-25167, dilution 1:1,000, in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at about 20kDa corresponds to the VLP1 protein.

Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody (3A9) [NBP2-25167] - Confocal image of adult rat cerebellum stained with NBP2-25167 (green), chicken polyclonal antibody to MAP2 NB300-213 (red) and DNA (blue). NBP2-25167 reveals perikarya and synaptic regions in the neuron rich granular layer (bottom) and synapse rich molecular layer (top). Note that the large prominent Purkinje neurons at the junction of these two layers do not stain with this antibody, in line with the finidngs of others.

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167] - Bovine cerebellum homogenate stained with NBP2-25167 in lane 11. Note the strong clean band running at 22 kDa. Lane 10 shows the same material stained with another antibody to visinin-like 1, NBP2-25166, which binds to the same band.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC
• Clone: 3A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

visinin-like 1 Antibody (3A9) Summary

• Immunogen: Full length human visinin-like 1 [UniProt# P62760]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VSNL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
• Application Notes: This visinin-like 1 (3A9) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~22 kDa.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for visinin-like 1 Antibody (3A9)

• HLP3Hippocalcin-like protein 3
• HPCAL3
• HUVISL1
• VILIP-1
• VILIPVLP-1
• visinin-like 1
• visinin-like protein 1
• VISL1

Background

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VSNL1
• Entrez:
  • 7447(Human)
• OMIM:
  • 600817(Human)
• Uniprot:
  • P62760(Human)