VEGFR3/Flt-4 Antibody (54733) [Phycoerythrin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R3/ Flt-4 PE-conjugated Monoclonal Antibody (Catalog # FAB3492P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 54733
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

VEGFR3/Flt-4 Antibody (54733) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VEGF R3/Flt-4
  Tyr25-Ile776
  Accession # P35916
• Specificity: Detects human VEGF R3/Flt-4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody (54733) [Phycoerythrin]

• EC 2.7.10
• EC 2.7.10.1
• FLT4
• Flt-4
• fms-related tyrosine kinase 4
• LMPH1A
• PCLFLT41
• soluble VEGFR3 variant 1
• soluble VEGFR3 variant 2
• soluble VEGFR3 variant 3
• Tyrosine-protein kinase receptor FLT4
• vascular endothelial growth factor receptor 3
• VEGF R3
• VEGFR3
• VEGFR-3
• VEGFR3Fms-like tyrosine kinase 4

Background

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGF R3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain, and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.

1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR3/Flt-4 Antibody (FAB3492P)(11)

Publications using FAB3492P

• David G.P. van IJzendoorn, Daniela C.F. Salvatori, Xu Cao, Francijna van den Hil, Inge H. Briaire-de Bruijn, Danielle de Jong et al. Vascular Tumor Recapitulated in Endothelial Cells from hiPSCs Engineered to Express the SERPINE1-FOSB Translocation Cell Reports Medicine 12/22/2020 [PMID: 33377124]
• Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth. Clin Cancer Res, 2015-01-23;21(7):1652-64. 2015-01-23 [PMID: 25617424] (Flow Cytometry, Human)
• Fiorio Pla , Alessand, Brossa , Alessia, Bernardini , Michela, Genova , Tullio, Grolez , Guillaum, Villers , Arnaud, Leroy , Xavier, Prevarskaya , Natalia, Gkika , Dimitra, Bussolati , Benedett Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. BMC Cancer, 2014-12-12;14(0):939. 2014-12-12 [PMID: 25494980] (Flow Cytometry, Human)
• Qiang Shi, Gerald Schatten, Vida Hodara, Calvin Simerly, John L. VandeBerg Endothelial reconstitution by CD34+ progenitors derived from baboon embryonic stem cells Journal of Cellular and Molecular Medicine 2/1/2013 [PMID: 23301772]
• Imoukhuede , Princess, Popel , Aleksand Expression of VEGF receptors on endothelial cells in mouse skeletal muscle. PLoS ONE, 2012-09-12;7(9):e44791. 2012-09-12 [PMID: 22984559] (Flow Cytometry, Mouse)
• Qiang Shi, Vida Hodara, Calvin R. Simerly, Gerald P. Schatten, John L. VandeBerg Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons Stem Cells and Development 2/15/2013 [PMID: 22931470]
• Qiang Shi, Laura A. Cox, Vida Hodara, Xing Li Wang, John L. VandeBerg Repertoire of endothelial progenitor cells mobilized by femoral artery ligation: a nonhuman primate study Journal of Cellular and Molecular Medicine 9/1/2012 [PMID: 22128816]
• Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells. J. Cell. Physiol., 2008-10-01;217(1):261-80. 2008-10-01 [PMID: 18551514] (Flow Cytometry, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Exp. Hematol., 2008-05-01;36(5):545-558. 2008-05-01 [PMID: 18439488] (Flow Cytometry, Human)
• Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression. J. Clin. Invest., 2007-11-01;117(11):3369-82. 2007-11-01 [PMID: 17948123] (Flow Cytometry, Human)
• Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007-04-30;177(3):489-500. 2007-04-30 [PMID: 17470632] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: FLT4
• Uniprot:
  • P35916()