VEGFR3/Flt-4 Antibody (54733) [Allophycocyanin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R3/ Flt-4 APC-conjugated Monoclonal Antibody (Catalog # FAB3492A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 54733
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

VEGFR3/Flt-4 Antibody (54733) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human VEGF R3/Flt-4
  Tyr25-Ile776
  Accession # P35916
• Specificity: Detects human VEGF R3/Flt-4 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR3/Flt-4 Antibody (54733) [Allophycocyanin]

• EC 2.7.10
• EC 2.7.10.1
• FLT4
• Flt-4
• fms-related tyrosine kinase 4
• LMPH1A
• PCLFLT41
• soluble VEGFR3 variant 1
• soluble VEGFR3 variant 2
• soluble VEGFR3 variant 3
• Tyrosine-protein kinase receptor FLT4
• vascular endothelial growth factor receptor 3
• VEGF R3
• VEGFR3
• VEGFR-3
• VEGFR3Fms-like tyrosine kinase 4

Background

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGF R3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain, and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.

1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VEGFR3/Flt-4 Antibody (FAB3492A)(8)

Publications using FAB3492A

• KL Betterman, DL Sutton, GA Secker, J Kazenwadel, A Oszmiana, L Lim, N Miura, L Sorokin, BM Hogan, ML Kahn, H McNeill, NL Harvey Atypical cadherin Fat4 orchestrates lymphatic endothelial cell polarity in response to flow J. Clin. Invest., 2020-06-01;0(0):. 2020-06-01 [PMID: 32182215] (Flow Cytometry, Human)
• M Witkowska, A Majchrzak, B Cebula-Obr, E Wawrzyniak, T Robak, P Smolewski The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia Tumour Biol, 2017-03-01;39(3):1010428317694. 2017-03-01 [PMID: 28349818] (Flow Cytometry, Human)
• Sie M, den Dunnen W, Lourens H, Meeuwsen-de Boer T, Scherpen F, Zomerman W, Kampen K, Hoving E, de Bont E Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma. PLoS ONE, 2015-03-23;10(3):e0122555. 2015-03-23 [PMID: 25799134] (Flow Cytometry, Human)
• Fiorio Pla , Alessand, Brossa , Alessia, Bernardini , Michela, Genova , Tullio, Grolez , Guillaum, Villers , Arnaud, Leroy , Xavier, Prevarskaya , Natalia, Gkika , Dimitra, Bussolati , Benedett Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. BMC Cancer, 2014-12-12;14(0):939. 2014-12-12 [PMID: 25494980] (Flow Cytometry, Human)
• Norton , Kerri-An, Han , Zheyi, Popel , Aleksand, Pandey , Niranjan Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells. Onco Targets Ther, 2014-09-09;7(0):1571-82. 2014-09-09 [PMID: 25228815] (Flow Cytometry, Human)
• Weidenaar A, Ter Elst A, Kampen K, Meeuwsen-de Boer T, Kamps W, Schuringa J, de Bont E Impaired long-term expansion and self-renewal potential of pediatric acute myeloid leukemia-initiating cells by PTK787/ZK 222584. Mol Cancer Res, 2013-02-07;11(4):339-48. 2013-02-07 [PMID: 23393162] (Flow Cytometry, Human)
• Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors. Oncogene, 2010-03-08;29(20):2938-49. 2010-03-08 [PMID: 20208561] (Flow Cytometry, Human)
• Roorda BD, Ter Elst A, Scherpen FJ, Meeuwsen-de Boer TG, Kamps WA, de Bont ES VEGF-A promotes lymphoma tumour growth by activation of STAT proteins and inhibition of p27(KIP1) via paracrine mechanisms. Eur. J. Cancer, 2010-01-22;46(5):974-82. 2010-01-22 [PMID: 20064707] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: FLT4
• Uniprot:
  • P35916()