VE-Statin/EGFL7 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VE-Statin/EGFL7 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse VE-Statin Isoform 1
  Thr21-Leu275
  Accession # Q9QXT5
• Specificity: Detects mouse VE-Statin Isoform 1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human VE-Statin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EGFL7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VE-Statin/EGFL7 Antibody [Unconjugated]

• EGFL7
• EGF-like protein 7
• EGF-like-domain, multiple 7
• epidermal growth factor-like protein 7
• MEGF7
• MGC111117
• Multiple EGF-like domains protein 7
• Multiple epidermal growth factor-like domains protein 7
• NEU1 protein
• NOTCH4-like protein
• RP11-251M1.2
• Vascular endothelial statin
• VEStatin
• VE-Statin
• ZNEU1

Background

Mouse VE-Statin (vascular endothelial cell statin; also EGFL7) is a secreted glycoprotein that belongs to an expanding family of EGF-like domain-containing proteins. It typically runs at 33-36 kDa in SDS-PAGE.  Higher molecular weight monomers ranging from 40-42 kDa have also been reported, and suggested to be a consequence of O-linked glycosylation.  VE-Statin is an early marker of embryonic endothelial cells, and occurs in adult endothelium. Its secretion blocks smooth muscle migration. The mature molecule is 254 amino acids (aa) in length, and contains one N-terminal EMI domain (aa 28‑105), two EGF-like domains (aa 106‑178), and a coiled‑coil region (aa 196‑220). There is at least one isoform that shows a 13 aa deletion between aa 236‑248. Mature mouse VE-statin shares 76% and 80% aa sequence identity with dog and human VE‑statin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VE-Statin/EGFL7 Antibody (AF3089)(3)

Publications using AF3089

• Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126 Development 2019-11-01 [PMID: 31582413] (Immunohistochemistry, Transgenic Mouse)
• Md. Riaj Mahamud, Xin Geng, Yen-Chun Ho, Boksik Cha, Yuenhee Kim, Jing Ma et al. GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126 Development 11/1/2019 [PMID: 31582413]
• N Dudvarski, F Bicker, S Keller, DT Jones, PN Harter, A Kienzle, C Gillmann, P Arnold, A Golebiewsk, O Keunen, A Giese, A von Deimli, T Bäuerle, SP Niclou, M Mittelbron, W Ye, SM Pfister, MH Schmidt EGFL7 enhances surface expression of integrin ?5?1 to promote angiogenesis in malignant brain tumors EMBO Mol Med, 2018-09-01;0(0):. 2018-09-01 [PMID: 30065025] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: EGFL7
• Uniprot:
  • Q9QXT5()